Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals
Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of co...
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2021
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ftucl:oai:eprints.ucl.ac.uk.OAI2:10131382 2023-12-24T10:24:02+01:00 Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH 2021-05-25 text https://discovery.ucl.ac.uk/id/eprint/10131382/1/41467_2021_Article_23216.pdf https://discovery.ucl.ac.uk/id/eprint/10131382/ eng eng NATURE RESEARCH https://discovery.ucl.ac.uk/id/eprint/10131382/1/41467_2021_Article_23216.pdf https://discovery.ucl.ac.uk/id/eprint/10131382/ open Nature Communications , 12 , Article 3117. (2021) Article 2021 ftucl 2023-11-27T13:07:35Z Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. Article in Journal/Newspaper Pacific oyster University College London: UCL Discovery Pacific |
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University College London: UCL Discovery |
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English |
description |
Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. |
format |
Article in Journal/Newspaper |
author |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH |
spellingShingle |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
author_facet |
Xu, F Marlétaz, F Gavriouchkina, D Liu, X Sauka-Spengler, T Zhang, G Holland, PWH |
author_sort |
Xu, F |
title |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_short |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_full |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_fullStr |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_full_unstemmed |
Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals |
title_sort |
evidence from oyster suggests an ancient role for pdx in regulating insulin gene expression in animals |
publisher |
NATURE RESEARCH |
publishDate |
2021 |
url |
https://discovery.ucl.ac.uk/id/eprint/10131382/1/41467_2021_Article_23216.pdf https://discovery.ucl.ac.uk/id/eprint/10131382/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Nature Communications , 12 , Article 3117. (2021) |
op_relation |
https://discovery.ucl.ac.uk/id/eprint/10131382/1/41467_2021_Article_23216.pdf https://discovery.ucl.ac.uk/id/eprint/10131382/ |
op_rights |
open |
_version_ |
1786198449502289920 |