H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved]
Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investi...
Main Authors: | , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
F1000 Research Ltd
2021
|
Subjects: | |
Online Access: | https://discovery.ucl.ac.uk/id/eprint/10123661/1/d1768cad-aa59-4c2a-a418-1b30596dc3ed_25019_-_andrew_w_liu_v2.pdf https://discovery.ucl.ac.uk/id/eprint/10123661/ |
id |
ftucl:oai:eprints.ucl.ac.uk.OAI2:10123661 |
---|---|
record_format |
openpolar |
spelling |
ftucl:oai:eprints.ucl.ac.uk.OAI2:10123661 2023-12-24T10:23:04+01:00 H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] Liu, AW Tan, Y Masunaga, A Bliznina, A West, C Plessy, C Luscombe, NM 2021-03-01 text https://discovery.ucl.ac.uk/id/eprint/10123661/1/d1768cad-aa59-4c2a-a418-1b30596dc3ed_25019_-_andrew_w_liu_v2.pdf https://discovery.ucl.ac.uk/id/eprint/10123661/ eng eng F1000 Research Ltd https://discovery.ucl.ac.uk/id/eprint/10123661/1/d1768cad-aa59-4c2a-a418-1b30596dc3ed_25019_-_andrew_w_liu_v2.pdf https://discovery.ucl.ac.uk/id/eprint/10123661/ open F1000Research , 9 , Article 780. (2021) karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P Article 2021 ftucl 2023-11-27T13:07:29Z Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investigations into marine ecosystems. The genome of north Atlantic O. dioica comprises three chromosomes. However, comparisons with the genomes of O. dioica sampled from mainland and southern Japan revealed extensive sequence differences. Moreover, historical studies have reported widely varying chromosome counts. We recently initiated a project to study the genomes of O. dioica individuals collected from the coastline of the Ryukyu (Okinawa) Islands in southern Japan. Given the potentially large extent of genomic diversity, we employed karyological techniques to count individual animals’ chromosomes in situ using centromere-specific antibodies directed against H3S28P, a prophase-metaphase cell cycle-specific marker of histone H3. Epifluorescence and confocal images were obtained of embryos and oocytes stained with two commercial anti-H3S28P antibodies (Abcam ab10543 and Thermo Fisher 07-145). The data lead us to conclude that diploid cells from Okinawan O. dioica contain three pairs of chromosomes, in line with the north Atlantic populations. The finding facilitates the telomere-to-telomere assembly of Okinawan O. dioica genome sequences and gives insight into the genomic diversity of O. dioica from different geographical locations. The data deposited in the EBI BioImage Archive provide representative images of the antibodies’ staining properties for use in epifluorescent and confocal based fluorescent microscopy. Article in Journal/Newspaper North Atlantic University College London: UCL Discovery |
institution |
Open Polar |
collection |
University College London: UCL Discovery |
op_collection_id |
ftucl |
language |
English |
topic |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P |
spellingShingle |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P Liu, AW Tan, Y Masunaga, A Bliznina, A West, C Plessy, C Luscombe, NM H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
topic_facet |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P |
description |
Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investigations into marine ecosystems. The genome of north Atlantic O. dioica comprises three chromosomes. However, comparisons with the genomes of O. dioica sampled from mainland and southern Japan revealed extensive sequence differences. Moreover, historical studies have reported widely varying chromosome counts. We recently initiated a project to study the genomes of O. dioica individuals collected from the coastline of the Ryukyu (Okinawa) Islands in southern Japan. Given the potentially large extent of genomic diversity, we employed karyological techniques to count individual animals’ chromosomes in situ using centromere-specific antibodies directed against H3S28P, a prophase-metaphase cell cycle-specific marker of histone H3. Epifluorescence and confocal images were obtained of embryos and oocytes stained with two commercial anti-H3S28P antibodies (Abcam ab10543 and Thermo Fisher 07-145). The data lead us to conclude that diploid cells from Okinawan O. dioica contain three pairs of chromosomes, in line with the north Atlantic populations. The finding facilitates the telomere-to-telomere assembly of Okinawan O. dioica genome sequences and gives insight into the genomic diversity of O. dioica from different geographical locations. The data deposited in the EBI BioImage Archive provide representative images of the antibodies’ staining properties for use in epifluorescent and confocal based fluorescent microscopy. |
format |
Article in Journal/Newspaper |
author |
Liu, AW Tan, Y Masunaga, A Bliznina, A West, C Plessy, C Luscombe, NM |
author_facet |
Liu, AW Tan, Y Masunaga, A Bliznina, A West, C Plessy, C Luscombe, NM |
author_sort |
Liu, AW |
title |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
title_short |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
title_full |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
title_fullStr |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
title_full_unstemmed |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes [version 2; peer review: 2 approved] |
title_sort |
h3s28p antibody staining of okinawan oikopleura dioica suggests the presence of three chromosomes [version 2; peer review: 2 approved] |
publisher |
F1000 Research Ltd |
publishDate |
2021 |
url |
https://discovery.ucl.ac.uk/id/eprint/10123661/1/d1768cad-aa59-4c2a-a418-1b30596dc3ed_25019_-_andrew_w_liu_v2.pdf https://discovery.ucl.ac.uk/id/eprint/10123661/ |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
F1000Research , 9 , Article 780. (2021) |
op_relation |
https://discovery.ucl.ac.uk/id/eprint/10123661/1/d1768cad-aa59-4c2a-a418-1b30596dc3ed_25019_-_andrew_w_liu_v2.pdf https://discovery.ucl.ac.uk/id/eprint/10123661/ |
op_rights |
open |
_version_ |
1786196772695048192 |