Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies
Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was condu...
Published in: | Journal of Phycology |
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Language: | English |
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2006
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Online Access: | https://hdl.handle.net/1983/7a40106b-6191-45b7-a616-847982df3165 https://research-information.bris.ac.uk/en/publications/7a40106b-6191-45b7-a616-847982df3165 https://doi.org/10.1111/j.1529-8817.2006.00231.x http://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2006.00231.x |
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ftubristolcris:oai:research-information.bris.ac.uk:publications/7a40106b-6191-45b7-a616-847982df3165 2024-02-04T10:03:02+01:00 Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies Iglesias-Rodriguez, MD Schofield, OM Batley, J Medlin, LK Hayes, PK 2006-06 https://hdl.handle.net/1983/7a40106b-6191-45b7-a616-847982df3165 https://research-information.bris.ac.uk/en/publications/7a40106b-6191-45b7-a616-847982df3165 https://doi.org/10.1111/j.1529-8817.2006.00231.x http://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2006.00231.x eng eng info:eu-repo/semantics/restrictedAccess Iglesias-Rodriguez , MD , Schofield , OM , Batley , J , Medlin , LK & Hayes , PK 2006 , ' Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies ' , Journal of Phycology , vol. 42 (3) , pp. 526 - 536 . https://doi.org/10.1111/j.1529-8817.2006.00231.x article 2006 ftubristolcris https://doi.org/10.1111/j.1529-8817.2006.00231.x 2024-01-11T23:36:24Z Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) (n=41) and a North East Atlantic (NEA) group (n=21), using FST as an indicator of genetic differentiation, revealed moderate genetic differentiation (FST=0.09894; P=0; significance level=0.05). Gene flow between the FJ92 and NEA groups was estimated to be low, which is in agreement with the moderate levels of genetic differentiation revealed by the microsatellite data. A genetic assignment method that uses genotype likelihoods to draw inference about the groups to which individuals belong was tested. Using FJ92 and NEA as reference groups, we observed that all the E. huxleyi groups tested against the two reference groups were unrelated to them. On a global biogeographical scale, E. huxleyi populations appear to be highly genetically diverse. Our findings raise the question of whether such a high degree of intraspecific genetic diversity in coccolithophores translates into variability in ecological function. Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) (n=41) and a North East Atlantic (NEA) group (n=21), using ... Article in Journal/Newspaper North East Atlantic University of Bristol: Bristol Research Journal of Phycology 42 3 526 536 |
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Open Polar |
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University of Bristol: Bristol Research |
op_collection_id |
ftubristolcris |
language |
English |
description |
Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) (n=41) and a North East Atlantic (NEA) group (n=21), using FST as an indicator of genetic differentiation, revealed moderate genetic differentiation (FST=0.09894; P=0; significance level=0.05). Gene flow between the FJ92 and NEA groups was estimated to be low, which is in agreement with the moderate levels of genetic differentiation revealed by the microsatellite data. A genetic assignment method that uses genotype likelihoods to draw inference about the groups to which individuals belong was tested. Using FJ92 and NEA as reference groups, we observed that all the E. huxleyi groups tested against the two reference groups were unrelated to them. On a global biogeographical scale, E. huxleyi populations appear to be highly genetically diverse. Our findings raise the question of whether such a high degree of intraspecific genetic diversity in coccolithophores translates into variability in ecological function. Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) (n=41) and a North East Atlantic (NEA) group (n=21), using ... |
format |
Article in Journal/Newspaper |
author |
Iglesias-Rodriguez, MD Schofield, OM Batley, J Medlin, LK Hayes, PK |
spellingShingle |
Iglesias-Rodriguez, MD Schofield, OM Batley, J Medlin, LK Hayes, PK Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
author_facet |
Iglesias-Rodriguez, MD Schofield, OM Batley, J Medlin, LK Hayes, PK |
author_sort |
Iglesias-Rodriguez, MD |
title |
Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
title_short |
Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
title_full |
Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
title_fullStr |
Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
title_full_unstemmed |
Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
title_sort |
intraspecific genetic diversity in the marine coccolithophore emiliania huxleyi (prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies |
publishDate |
2006 |
url |
https://hdl.handle.net/1983/7a40106b-6191-45b7-a616-847982df3165 https://research-information.bris.ac.uk/en/publications/7a40106b-6191-45b7-a616-847982df3165 https://doi.org/10.1111/j.1529-8817.2006.00231.x http://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2006.00231.x |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_source |
Iglesias-Rodriguez , MD , Schofield , OM , Batley , J , Medlin , LK & Hayes , PK 2006 , ' Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies ' , Journal of Phycology , vol. 42 (3) , pp. 526 - 536 . https://doi.org/10.1111/j.1529-8817.2006.00231.x |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1529-8817.2006.00231.x |
container_title |
Journal of Phycology |
container_volume |
42 |
container_issue |
3 |
container_start_page |
526 |
op_container_end_page |
536 |
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1789970156957466624 |