Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...
Published in: | FEMS Microbiology Ecology |
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Language: | English |
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2018
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Online Access: | https://hdl.handle.net/1983/6290fe83-f838-4487-8448-bd08c6e2b237 https://research-information.bris.ac.uk/en/publications/6290fe83-f838-4487-8448-bd08c6e2b237 https://doi.org/10.1093/femsec/fiy114 https://research-information.bris.ac.uk/ws/files/164646387/fiy114.pdf http://www.scopus.com/inward/record.url?scp=85050825953&partnerID=8YFLogxK |
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ftubristolcris:oai:research-information.bris.ac.uk:publications/6290fe83-f838-4487-8448-bd08c6e2b237 2024-01-07T09:40:35+01:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia 2018-09-01 application/pdf https://hdl.handle.net/1983/6290fe83-f838-4487-8448-bd08c6e2b237 https://research-information.bris.ac.uk/en/publications/6290fe83-f838-4487-8448-bd08c6e2b237 https://doi.org/10.1093/femsec/fiy114 https://research-information.bris.ac.uk/ws/files/164646387/fiy114.pdf http://www.scopus.com/inward/record.url?scp=85050825953&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Nash , M V , Anesio , A M , Barker , G , Tranter , M , Varliero , G , Eloe-Fadrosh , E A , Nielsen , T , Turpin-Jelfs , T , Benning , L G & Sánchez-Baracaldo , P 2018 , ' Metagenomic insights into diazotrophic communities across Arctic glacier forefields ' , FEMS Microbiology Ecology , vol. 94 , no. 9 , fiy114 . https://doi.org/10.1093/femsec/fiy114 , https://doi.org/10.1093/femsec/fiy114 Nitrogen fixation Metagenomics Forefield Arctic Diversity Diazotrophs article 2018 ftubristolcris https://doi.org/10.1093/femsec/fiy114 2023-12-14T23:32:22Z Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Article in Journal/Newspaper Arctic Arctic University of Bristol: Bristol Research Arctic FEMS Microbiology Ecology 94 9 |
institution |
Open Polar |
collection |
University of Bristol: Bristol Research |
op_collection_id |
ftubristolcris |
language |
English |
topic |
Nitrogen fixation Metagenomics Forefield Arctic Diversity Diazotrophs |
spellingShingle |
Nitrogen fixation Metagenomics Forefield Arctic Diversity Diazotrophs Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
topic_facet |
Nitrogen fixation Metagenomics Forefield Arctic Diversity Diazotrophs |
description |
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
format |
Article in Journal/Newspaper |
author |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia |
author_facet |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia |
author_sort |
Nash, Maisie V. |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publishDate |
2018 |
url |
https://hdl.handle.net/1983/6290fe83-f838-4487-8448-bd08c6e2b237 https://research-information.bris.ac.uk/en/publications/6290fe83-f838-4487-8448-bd08c6e2b237 https://doi.org/10.1093/femsec/fiy114 https://research-information.bris.ac.uk/ws/files/164646387/fiy114.pdf http://www.scopus.com/inward/record.url?scp=85050825953&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_source |
Nash , M V , Anesio , A M , Barker , G , Tranter , M , Varliero , G , Eloe-Fadrosh , E A , Nielsen , T , Turpin-Jelfs , T , Benning , L G & Sánchez-Baracaldo , P 2018 , ' Metagenomic insights into diazotrophic communities across Arctic glacier forefields ' , FEMS Microbiology Ecology , vol. 94 , no. 9 , fiy114 . https://doi.org/10.1093/femsec/fiy114 , https://doi.org/10.1093/femsec/fiy114 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/femsec/fiy114 |
container_title |
FEMS Microbiology Ecology |
container_volume |
94 |
container_issue |
9 |
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1787421443430023168 |