Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to me...
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Online Access: | https://hdl.handle.net/1983/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://research-information.bris.ac.uk/en/publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://doi.org/10.1038/s41598-019-55579-9 https://research-information.bris.ac.uk/ws/files/224288337/s41598_019_55579_9.pdf |
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ftubristolcris:oai:research-information.bris.ac.uk:publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac 2024-04-28T08:16:13+00:00 Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis Hoettges, Kai F Henslee, Erin Torcal Serrano, Ruth M Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Chris H van der Veen, Daan Labeed, Fatima H Hughes, Michael 2019-12-16 application/pdf https://hdl.handle.net/1983/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://research-information.bris.ac.uk/en/publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://doi.org/10.1038/s41598-019-55579-9 https://research-information.bris.ac.uk/ws/files/224288337/s41598_019_55579_9.pdf eng eng https://research-information.bris.ac.uk/en/publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac info:eu-repo/semantics/openAccess Hoettges , K F , Henslee , E , Torcal Serrano , R M , Jabr , R , Abdallat , R , Beale , A , Waheed , A , Camelliti , P , Fry , C H , van der Veen , D , Labeed , F H & Hughes , M 2019 , ' Ten–Second Electrophysiology : Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis ' , Scientific Reports , vol. 9 , 19153 (2019) . https://doi.org/10.1038/s41598-019-55579-9 Dielectrophoresis Electrorotation DEP electrokinetics membrane article 2019 ftubristolcris https://doi.org/10.1038/s41598-019-55579-9 2024-04-10T00:11:18Z Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2>0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2-3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. Article in Journal/Newspaper Common vole Microtus arvalis University of Bristol: Bristol Research Scientific Reports 9 1 |
institution |
Open Polar |
collection |
University of Bristol: Bristol Research |
op_collection_id |
ftubristolcris |
language |
English |
topic |
Dielectrophoresis Electrorotation DEP electrokinetics membrane |
spellingShingle |
Dielectrophoresis Electrorotation DEP electrokinetics membrane Hoettges, Kai F Henslee, Erin Torcal Serrano, Ruth M Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Chris H van der Veen, Daan Labeed, Fatima H Hughes, Michael Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
topic_facet |
Dielectrophoresis Electrorotation DEP electrokinetics membrane |
description |
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2>0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2-3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. |
format |
Article in Journal/Newspaper |
author |
Hoettges, Kai F Henslee, Erin Torcal Serrano, Ruth M Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Chris H van der Veen, Daan Labeed, Fatima H Hughes, Michael |
author_facet |
Hoettges, Kai F Henslee, Erin Torcal Serrano, Ruth M Jabr, Rita Abdallat, Rula Beale, Andrew Waheed, Abdul Camelliti, Patrizia Fry, Chris H van der Veen, Daan Labeed, Fatima H Hughes, Michael |
author_sort |
Hoettges, Kai F |
title |
Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_short |
Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full |
Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_fullStr |
Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full_unstemmed |
Ten–Second Electrophysiology:Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_sort |
ten–second electrophysiology:evaluation of the 3dep platform for high-speed, high-accuracy cell analysis |
publishDate |
2019 |
url |
https://hdl.handle.net/1983/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://research-information.bris.ac.uk/en/publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac https://doi.org/10.1038/s41598-019-55579-9 https://research-information.bris.ac.uk/ws/files/224288337/s41598_019_55579_9.pdf |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Hoettges , K F , Henslee , E , Torcal Serrano , R M , Jabr , R , Abdallat , R , Beale , A , Waheed , A , Camelliti , P , Fry , C H , van der Veen , D , Labeed , F H & Hughes , M 2019 , ' Ten–Second Electrophysiology : Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis ' , Scientific Reports , vol. 9 , 19153 (2019) . https://doi.org/10.1038/s41598-019-55579-9 |
op_relation |
https://research-information.bris.ac.uk/en/publications/0cbb0ee2-5a93-43da-8895-76cda21df0ac |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41598-019-55579-9 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1797581397369552896 |