Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals
The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | https://publishup.uni-potsdam.de/frontdoor/index/index/docId/61245 https://doi.org/10.1038/s41598-022-21658-7 |
id |
ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:61245 |
---|---|
record_format |
openpolar |
spelling |
ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:61245 2023-12-10T09:42:32+01:00 Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals Tebbe, Jonas (Dr.) Ottensmann, Meinolf Havenstein, Katja Efstratiou, Artemis Lenz, Tobias L. (Prof. Dr.) Caspers, Barbara A. (Prof. Dr.) Forcada, Jaume Tiedemann, Ralph (Prof. Dr.) Hoffman, Joseph (Prof. Dr.) 2022-10-26 https://publishup.uni-potsdam.de/frontdoor/index/index/docId/61245 https://doi.org/10.1038/s41598-022-21658-7 eng eng https://publishup.uni-potsdam.de/frontdoor/index/index/docId/61245 https://doi.org/10.1038/s41598-022-21658-7 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/closedAccess ddc:570 Institut für Biochemie und Biologie article doc-type:article 2022 ftubpotsdam https://doi.org/10.1038/s41598-022-21658-7 2023-11-12T23:35:12Z The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional polymorphism of MHC genes, careful PCR primer design is crucial for capturing all of the allelic variation present in a given species. We therefore developed intronic primers to amplify the full-length 267 bp protein-coding sequence of the MHC class II DQB exon 2 in the Antarctic fur seal. We then characterised patterns of MHC variation among mother-offspring pairs from two breeding colonies and detected 19 alleles among 771 clone sequences from 56 individuals. The distribution of alleles within and among individuals was consistent with a single-copy, classical DQB locus showing Mendelian inheritance. Amino acid similarity at the MHC was significantly associated with genome-wide relatedness, but no relationship was found between MHC heterozygosity and genome-wide heterozygosity. Finally, allelic diversity was several times higher than reported by a previous study based on partial exon sequences. This difference appears to be related to allele-specific amplification bias, implying that primer design can strongly impact the inference of MHC diversity. Article in Journal/Newspaper Antarc* Antarctic Antarctic Fur Seal Antarctic Fur Seals University of Potsdam: publish.UP Antarctic The Antarctic Scientific Reports 12 1 |
institution |
Open Polar |
collection |
University of Potsdam: publish.UP |
op_collection_id |
ftubpotsdam |
language |
English |
topic |
ddc:570 Institut für Biochemie und Biologie |
spellingShingle |
ddc:570 Institut für Biochemie und Biologie Tebbe, Jonas (Dr.) Ottensmann, Meinolf Havenstein, Katja Efstratiou, Artemis Lenz, Tobias L. (Prof. Dr.) Caspers, Barbara A. (Prof. Dr.) Forcada, Jaume Tiedemann, Ralph (Prof. Dr.) Hoffman, Joseph (Prof. Dr.) Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
topic_facet |
ddc:570 Institut für Biochemie und Biologie |
description |
The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional polymorphism of MHC genes, careful PCR primer design is crucial for capturing all of the allelic variation present in a given species. We therefore developed intronic primers to amplify the full-length 267 bp protein-coding sequence of the MHC class II DQB exon 2 in the Antarctic fur seal. We then characterised patterns of MHC variation among mother-offspring pairs from two breeding colonies and detected 19 alleles among 771 clone sequences from 56 individuals. The distribution of alleles within and among individuals was consistent with a single-copy, classical DQB locus showing Mendelian inheritance. Amino acid similarity at the MHC was significantly associated with genome-wide relatedness, but no relationship was found between MHC heterozygosity and genome-wide heterozygosity. Finally, allelic diversity was several times higher than reported by a previous study based on partial exon sequences. This difference appears to be related to allele-specific amplification bias, implying that primer design can strongly impact the inference of MHC diversity. |
format |
Article in Journal/Newspaper |
author |
Tebbe, Jonas (Dr.) Ottensmann, Meinolf Havenstein, Katja Efstratiou, Artemis Lenz, Tobias L. (Prof. Dr.) Caspers, Barbara A. (Prof. Dr.) Forcada, Jaume Tiedemann, Ralph (Prof. Dr.) Hoffman, Joseph (Prof. Dr.) |
author_facet |
Tebbe, Jonas (Dr.) Ottensmann, Meinolf Havenstein, Katja Efstratiou, Artemis Lenz, Tobias L. (Prof. Dr.) Caspers, Barbara A. (Prof. Dr.) Forcada, Jaume Tiedemann, Ralph (Prof. Dr.) Hoffman, Joseph (Prof. Dr.) |
author_sort |
Tebbe, Jonas (Dr.) |
title |
Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
title_short |
Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
title_full |
Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
title_fullStr |
Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
title_full_unstemmed |
Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals |
title_sort |
intronic primers reveal unexpectedly high major histocompatibility complex diversity in antarctic fur seals |
publishDate |
2022 |
url |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/61245 https://doi.org/10.1038/s41598-022-21658-7 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Fur Seal Antarctic Fur Seals |
genre_facet |
Antarc* Antarctic Antarctic Fur Seal Antarctic Fur Seals |
op_relation |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/61245 https://doi.org/10.1038/s41598-022-21658-7 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1038/s41598-022-21658-7 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1784885412920033280 |