Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic
Lakes cover large parts of the climatically sensitive Arctic landscape and respond rapidly to environmental change. Arctic lakes have different origins and include the predominant thermokarst lakes, which are small, young and highly dynamic, as well as large, old and stable glacial lakes. Freshwater...
Published in: | Journal of Paleolimnology |
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2020
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Online Access: | https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60532 https://doi.org/10.1007/s10933-020-00133-1 |
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ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:60532 2023-12-17T10:25:24+01:00 Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic Huang, Sichao Herzschuh, Ulrike (Prof. Dr.) Pestryakova, Luidmila Agafyevna Zimmermann, Heike Hildegard Davydova, Paraskovya Biskaborn, Boris K. Shevtsova, Iuliia Stoof-Leichsenring, Kathleen Rosemarie (Dr.) 2020-05-30 https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60532 https://doi.org/10.1007/s10933-020-00133-1 eng eng https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60532 https://doi.org/10.1007/s10933-020-00133-1 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/closedAccess ddc:550 ddc:570 Institut für Biochemie und Biologie Institut für Geowissenschaften article doc-type:article 2020 ftubpotsdam https://doi.org/10.1007/s10933-020-00133-1 2023-11-19T23:35:20Z Lakes cover large parts of the climatically sensitive Arctic landscape and respond rapidly to environmental change. Arctic lakes have different origins and include the predominant thermokarst lakes, which are small, young and highly dynamic, as well as large, old and stable glacial lakes. Freshwater diatoms dominate the primary producer community in these lakes and can be used to detect biotic responses to climate and environmental change. We used specific diatom metabarcoding on sedimentary DNA, combined with next-generation sequencing and diatom morphology, to assess diatom diversity in five glacial and 15 thermokarst lakes within the easternmost expanse of the Siberian treeline ecotone in Chukotka, Russia. We obtained 163 verified diatom sequence types and identified 176 diatom species morphologically. Although there were large differences in taxonomic assignment using the two approaches, they showed similar high abundances and diversity of Fragilariceae and Aulacoseiraceae. In particular, the genetic approach detected hidden within-lake variations of fragilarioids in glacial lakes and dominance of centric Aulacoseira species, whereas Lindavia ocellata was predominant using morphology. In thermokarst lakes, sequence types and valve counts also detected high diversity of Fragilariaceae, which followed the vegetation gradient along the treeline. Ordination analyses of the genetic data from glacial and thermokarst lakes suggest that concentrations of sulfate (SO42-), an indicator of the activity of sulfate-reducing microbes under anoxic conditions, and bicarbonate (HCO3-), which relates to surrounding vegetation, have a significant influence on diatom community composition. For thermokarst lakes, we also identified lake depth as an important variable, but SO42- best explains diatom diversity derived from genetic data, whereas HCO3- best explains the data from valve counts. Higher diatom diversity was detected in glacial lakes, most likely related to greater lake age and different edaphic settings, which gave ... Article in Journal/Newspaper Arctic Chukotka Thermokarst University of Potsdam: publish.UP Arctic Journal of Paleolimnology 64 3 225 242 |
institution |
Open Polar |
collection |
University of Potsdam: publish.UP |
op_collection_id |
ftubpotsdam |
language |
English |
topic |
ddc:550 ddc:570 Institut für Biochemie und Biologie Institut für Geowissenschaften |
spellingShingle |
ddc:550 ddc:570 Institut für Biochemie und Biologie Institut für Geowissenschaften Huang, Sichao Herzschuh, Ulrike (Prof. Dr.) Pestryakova, Luidmila Agafyevna Zimmermann, Heike Hildegard Davydova, Paraskovya Biskaborn, Boris K. Shevtsova, Iuliia Stoof-Leichsenring, Kathleen Rosemarie (Dr.) Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
topic_facet |
ddc:550 ddc:570 Institut für Biochemie und Biologie Institut für Geowissenschaften |
description |
Lakes cover large parts of the climatically sensitive Arctic landscape and respond rapidly to environmental change. Arctic lakes have different origins and include the predominant thermokarst lakes, which are small, young and highly dynamic, as well as large, old and stable glacial lakes. Freshwater diatoms dominate the primary producer community in these lakes and can be used to detect biotic responses to climate and environmental change. We used specific diatom metabarcoding on sedimentary DNA, combined with next-generation sequencing and diatom morphology, to assess diatom diversity in five glacial and 15 thermokarst lakes within the easternmost expanse of the Siberian treeline ecotone in Chukotka, Russia. We obtained 163 verified diatom sequence types and identified 176 diatom species morphologically. Although there were large differences in taxonomic assignment using the two approaches, they showed similar high abundances and diversity of Fragilariceae and Aulacoseiraceae. In particular, the genetic approach detected hidden within-lake variations of fragilarioids in glacial lakes and dominance of centric Aulacoseira species, whereas Lindavia ocellata was predominant using morphology. In thermokarst lakes, sequence types and valve counts also detected high diversity of Fragilariaceae, which followed the vegetation gradient along the treeline. Ordination analyses of the genetic data from glacial and thermokarst lakes suggest that concentrations of sulfate (SO42-), an indicator of the activity of sulfate-reducing microbes under anoxic conditions, and bicarbonate (HCO3-), which relates to surrounding vegetation, have a significant influence on diatom community composition. For thermokarst lakes, we also identified lake depth as an important variable, but SO42- best explains diatom diversity derived from genetic data, whereas HCO3- best explains the data from valve counts. Higher diatom diversity was detected in glacial lakes, most likely related to greater lake age and different edaphic settings, which gave ... |
format |
Article in Journal/Newspaper |
author |
Huang, Sichao Herzschuh, Ulrike (Prof. Dr.) Pestryakova, Luidmila Agafyevna Zimmermann, Heike Hildegard Davydova, Paraskovya Biskaborn, Boris K. Shevtsova, Iuliia Stoof-Leichsenring, Kathleen Rosemarie (Dr.) |
author_facet |
Huang, Sichao Herzschuh, Ulrike (Prof. Dr.) Pestryakova, Luidmila Agafyevna Zimmermann, Heike Hildegard Davydova, Paraskovya Biskaborn, Boris K. Shevtsova, Iuliia Stoof-Leichsenring, Kathleen Rosemarie (Dr.) |
author_sort |
Huang, Sichao |
title |
Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
title_short |
Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
title_full |
Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
title_fullStr |
Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
title_full_unstemmed |
Genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the Siberian Arctic |
title_sort |
genetic and morphologic determination of diatom community composition in surface sediments from glacial and thermokarst lakes in the siberian arctic |
publishDate |
2020 |
url |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60532 https://doi.org/10.1007/s10933-020-00133-1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Chukotka Thermokarst |
genre_facet |
Arctic Chukotka Thermokarst |
op_relation |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60532 https://doi.org/10.1007/s10933-020-00133-1 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s10933-020-00133-1 |
container_title |
Journal of Paleolimnology |
container_volume |
64 |
container_issue |
3 |
container_start_page |
225 |
op_container_end_page |
242 |
_version_ |
1785576332890996736 |