Hydrogen Utilization Potential in Subsurface Sediments
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pa...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/45679 https://doi.org/10.3389/fmicb.2016.00008 |
id |
ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:45679 |
---|---|
record_format |
openpolar |
spelling |
ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:45679 2023-05-15T15:39:01+02:00 Hydrogen Utilization Potential in Subsurface Sediments Adhikari, Rishi Ram Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. Kallmeyer, Jens (Dr.) 2016 https://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/45679 https://doi.org/10.3389/fmicb.2016.00008 eng eng https://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/45679 https://doi.org/10.3389/fmicb.2016.00008 info:eu-repo/semantics/closedAccess Institut für Geowissenschaften article doc-type:article 2016 ftubpotsdam https://doi.org/10.3389/fmicb.2016.00008 2022-07-28T20:48:54Z Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific, and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product, or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material. We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H(2)ases to successively higher concentrations of H-2 in successively deeper zones. Article in Journal/Newspaper Barents Sea University of Potsdam: publish.UP Barents Sea Pacific Frontiers in Microbiology 7 |
institution |
Open Polar |
collection |
University of Potsdam: publish.UP |
op_collection_id |
ftubpotsdam |
language |
English |
topic |
Institut für Geowissenschaften |
spellingShingle |
Institut für Geowissenschaften Adhikari, Rishi Ram Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. Kallmeyer, Jens (Dr.) Hydrogen Utilization Potential in Subsurface Sediments |
topic_facet |
Institut für Geowissenschaften |
description |
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific, and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product, or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material. We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H(2)ases to successively higher concentrations of H-2 in successively deeper zones. |
format |
Article in Journal/Newspaper |
author |
Adhikari, Rishi Ram Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. Kallmeyer, Jens (Dr.) |
author_facet |
Adhikari, Rishi Ram Glombitza, Clemens Nickel, Julia C. Anderson, Chloe H. Dunlea, Ann G. Spivack, Arthur J. Murray, Richard W. Kallmeyer, Jens (Dr.) |
author_sort |
Adhikari, Rishi Ram |
title |
Hydrogen Utilization Potential in Subsurface Sediments |
title_short |
Hydrogen Utilization Potential in Subsurface Sediments |
title_full |
Hydrogen Utilization Potential in Subsurface Sediments |
title_fullStr |
Hydrogen Utilization Potential in Subsurface Sediments |
title_full_unstemmed |
Hydrogen Utilization Potential in Subsurface Sediments |
title_sort |
hydrogen utilization potential in subsurface sediments |
publishDate |
2016 |
url |
https://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/45679 https://doi.org/10.3389/fmicb.2016.00008 |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_relation |
https://publishup.uni-potsdam.de/opus4-ubp/frontdoor/index/index/docId/45679 https://doi.org/10.3389/fmicb.2016.00008 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.3389/fmicb.2016.00008 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
_version_ |
1766370467979984896 |