Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments
The existence of diverse and active microbial ecosystems in the deep subsurface – a biosphere that was originally considered devoid of life – was discovered in multiple microbiological studies. However, most of the studies are restricted to marine ecosystems, while our knowledge about the microbial...
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ftubpotsdam:oai:kobv.de-opus4-uni-potsdam:40343 2023-07-02T03:31:35+02:00 Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments Phylogenetische und physiologische Charakterisierung der Tiefen Biosphäre in El'gygytgyn Kraterseesedimenten Heise, Janine 2017 application/pdf https://publishup.uni-potsdam.de/frontdoor/index/index/docId/40343 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-403436 https://publishup.uni-potsdam.de/files/40343/heise_diss.pdf eng eng https://publishup.uni-potsdam.de/frontdoor/index/index/docId/40343 urn:nbn:de:kobv:517-opus4-403436 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-403436 https://publishup.uni-potsdam.de/files/40343/heise_diss.pdf https://rightsstatements.org/vocab/InC/1.0/ info:eu-repo/semantics/openAccess ddc:570 Institut für Biochemie und Biologie doctoralthesis doc-type:doctoralThesis 2017 ftubpotsdam 2023-06-11T22:36:20Z The existence of diverse and active microbial ecosystems in the deep subsurface – a biosphere that was originally considered devoid of life – was discovered in multiple microbiological studies. However, most of the studies are restricted to marine ecosystems, while our knowledge about the microbial communities in the deep subsurface of lake systems and their potentials to adapt to changing environmental conditions is still fragmentary. This doctoral thesis aims to build up a unique data basis for providing the first detailed high-throughput characterization of the deep biosphere of lacustrine sediments and to emphasize how important it is to differentiate between the living and the dead microbial community in deep biosphere studies. In this thesis, up to 3.6 Ma old sediments (up to 317 m deep) of the El’gygytgyn Crater Lake were examined, which represents the oldest terrestrial climate record of the Arctic. Combining next generation sequencing with detailed geochemical characteristics and other environmental parameters, the microbial community composition was analyzed in regard to changing climatic conditions within the last 3.6 Ma to 1.0 Ma (Pliocene and Pleistocene). DNA from all investigated sediments was successfully extracted and a surprisingly diverse (6,910 OTUs) and abundant microbial community in the El’gygytgyn deep sediments were revealed. The bacterial abundance (10³-10⁶ 16S rRNA copies g⁻¹ sediment) was up to two orders of magnitudes higher than the archaeal abundance (10¹-10⁵) and fluctuates with the Pleistocene glacial/interglacial cyclicality. Interestingly, a strong increase in the microbial diversity with depth was observed (approximately 2.5 times higher diversity in Pliocene sediments compared to Pleistocene sediments). The increase in diversity with depth in the Lake El’gygytgyn is most probably caused by higher sedimentary temperatures towards the deep sediment layers as well as an enhanced temperature-induced intra-lake bioproductivity and higher input of allochthonous organic-rich ... Doctoral or Postdoctoral Thesis Arctic University of Potsdam: publish.UP Arctic Crater Lake ENVELOPE(-60.667,-60.667,-62.983,-62.983) |
institution |
Open Polar |
collection |
University of Potsdam: publish.UP |
op_collection_id |
ftubpotsdam |
language |
English |
topic |
ddc:570 Institut für Biochemie und Biologie |
spellingShingle |
ddc:570 Institut für Biochemie und Biologie Heise, Janine Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
topic_facet |
ddc:570 Institut für Biochemie und Biologie |
description |
The existence of diverse and active microbial ecosystems in the deep subsurface – a biosphere that was originally considered devoid of life – was discovered in multiple microbiological studies. However, most of the studies are restricted to marine ecosystems, while our knowledge about the microbial communities in the deep subsurface of lake systems and their potentials to adapt to changing environmental conditions is still fragmentary. This doctoral thesis aims to build up a unique data basis for providing the first detailed high-throughput characterization of the deep biosphere of lacustrine sediments and to emphasize how important it is to differentiate between the living and the dead microbial community in deep biosphere studies. In this thesis, up to 3.6 Ma old sediments (up to 317 m deep) of the El’gygytgyn Crater Lake were examined, which represents the oldest terrestrial climate record of the Arctic. Combining next generation sequencing with detailed geochemical characteristics and other environmental parameters, the microbial community composition was analyzed in regard to changing climatic conditions within the last 3.6 Ma to 1.0 Ma (Pliocene and Pleistocene). DNA from all investigated sediments was successfully extracted and a surprisingly diverse (6,910 OTUs) and abundant microbial community in the El’gygytgyn deep sediments were revealed. The bacterial abundance (10³-10⁶ 16S rRNA copies g⁻¹ sediment) was up to two orders of magnitudes higher than the archaeal abundance (10¹-10⁵) and fluctuates with the Pleistocene glacial/interglacial cyclicality. Interestingly, a strong increase in the microbial diversity with depth was observed (approximately 2.5 times higher diversity in Pliocene sediments compared to Pleistocene sediments). The increase in diversity with depth in the Lake El’gygytgyn is most probably caused by higher sedimentary temperatures towards the deep sediment layers as well as an enhanced temperature-induced intra-lake bioproductivity and higher input of allochthonous organic-rich ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Heise, Janine |
author_facet |
Heise, Janine |
author_sort |
Heise, Janine |
title |
Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
title_short |
Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
title_full |
Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
title_fullStr |
Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
title_full_unstemmed |
Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments |
title_sort |
phylogenetic and physiological characterization of deep-biosphere microorganisms in el’gygytgyn crater lake sediments |
publishDate |
2017 |
url |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/40343 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-403436 https://publishup.uni-potsdam.de/files/40343/heise_diss.pdf |
long_lat |
ENVELOPE(-60.667,-60.667,-62.983,-62.983) |
geographic |
Arctic Crater Lake |
geographic_facet |
Arctic Crater Lake |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/40343 urn:nbn:de:kobv:517-opus4-403436 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-403436 https://publishup.uni-potsdam.de/files/40343/heise_diss.pdf |
op_rights |
https://rightsstatements.org/vocab/InC/1.0/ info:eu-repo/semantics/openAccess |
_version_ |
1770270950167674880 |