Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica
Cyanobacteria are known to produce hepatotoxic substances, the functional and ecological role of these toxins, however, remains largely unclear. Toxic properties of cyanobacteria collected in Antarctica were investigated to determine whether toxin-producing species can also be found under these envi...
Published in: | Toxicon |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2000
|
Subjects: | |
Online Access: | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50339 https://doi.org/10.1016/S0041-0101(00)00103-3 |
id |
ftubkonstanz:oai:kops.uni-konstanz.de:123456789/8778 |
---|---|
record_format |
openpolar |
spelling |
ftubkonstanz:oai:kops.uni-konstanz.de:123456789/8778 2024-02-11T09:58:53+01:00 Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica Hitzfeld, Bettina C. Lampert, Christina S. Späth, Nicola Mountfort, Doug Kaspar, Henry Dietrich, Daniel R. 2000 application/pdf http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50339 https://doi.org/10.1016/S0041-0101(00)00103-3 eng eng http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50339 http://dx.doi.org/10.1016/S0041-0101(00)00103-3 278033180 http://creativecommons.org/licenses/by-nc-nd/2.0/ Toxicon. 2000, 38(12), pp. 1731-1748. ISSN 0041-0101. Available under: doi:10.1016/S0041-0101(00)00103-3 ddc:570 doc-type:article doc-type:Text 2000 ftubkonstanz https://doi.org/10.1016/S0041-0101(00)00103-3 2024-01-21T23:56:23Z Cyanobacteria are known to produce hepatotoxic substances, the functional and ecological role of these toxins, however, remains largely unclear. Toxic properties of cyanobacteria collected in Antarctica were investigated to determine whether toxin-producing species can also be found under these environmental conditions. Samples were collected from meltwater ponds on the McMurdo Ice Shelf, Antarctica in the summers of 1997 to 1999. These ponds are colonized by benthic algae and cyanobacterial mats. Oscillatoriales, Nodularia sp., and Nostoc sp. constituted the major taxa in freshwater ponds, while Nostoc sp. was missing from brackish and saline ponds. Samples were taken from either floating, submerged or benthic mats, and extracted for in vitro toxicity testing. The presence of toxins was determined by the phosphatase-inhibition assay and by high performance liquid chromatography. The cytotoxic properties of the extracts were investigated in hepatocytes determining 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide metabolism and trypan blue dye exclusion. The results show that all cyanobacterial extracts display phosphatase-inhibiting activity, of which approximately half had significantly greater than 50% inhibiting activity. The presence of nodularin and microcystin-LR was established by high performance liquid chromatography. Cytotoxic properties, independent of the phosphatase inhibiting activity, were also detected. Toxic strains of cyanobacteria can therefore also be found in Antarctica and this finding may lead to further insight into potential ecological roles of cyanobacterial phosphatase inhibiting toxins. published published Article in Journal/Newspaper Antarc* Antarctica Ice Shelf McMurdo Ice Shelf KOPS - The Institutional Repository of the University of Konstanz McMurdo Ice Shelf ENVELOPE(166.500,166.500,-78.000,-78.000) Toxicon 38 12 1731 1748 |
institution |
Open Polar |
collection |
KOPS - The Institutional Repository of the University of Konstanz |
op_collection_id |
ftubkonstanz |
language |
English |
topic |
ddc:570 |
spellingShingle |
ddc:570 Hitzfeld, Bettina C. Lampert, Christina S. Späth, Nicola Mountfort, Doug Kaspar, Henry Dietrich, Daniel R. Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
topic_facet |
ddc:570 |
description |
Cyanobacteria are known to produce hepatotoxic substances, the functional and ecological role of these toxins, however, remains largely unclear. Toxic properties of cyanobacteria collected in Antarctica were investigated to determine whether toxin-producing species can also be found under these environmental conditions. Samples were collected from meltwater ponds on the McMurdo Ice Shelf, Antarctica in the summers of 1997 to 1999. These ponds are colonized by benthic algae and cyanobacterial mats. Oscillatoriales, Nodularia sp., and Nostoc sp. constituted the major taxa in freshwater ponds, while Nostoc sp. was missing from brackish and saline ponds. Samples were taken from either floating, submerged or benthic mats, and extracted for in vitro toxicity testing. The presence of toxins was determined by the phosphatase-inhibition assay and by high performance liquid chromatography. The cytotoxic properties of the extracts were investigated in hepatocytes determining 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide metabolism and trypan blue dye exclusion. The results show that all cyanobacterial extracts display phosphatase-inhibiting activity, of which approximately half had significantly greater than 50% inhibiting activity. The presence of nodularin and microcystin-LR was established by high performance liquid chromatography. Cytotoxic properties, independent of the phosphatase inhibiting activity, were also detected. Toxic strains of cyanobacteria can therefore also be found in Antarctica and this finding may lead to further insight into potential ecological roles of cyanobacterial phosphatase inhibiting toxins. published published |
format |
Article in Journal/Newspaper |
author |
Hitzfeld, Bettina C. Lampert, Christina S. Späth, Nicola Mountfort, Doug Kaspar, Henry Dietrich, Daniel R. |
author_facet |
Hitzfeld, Bettina C. Lampert, Christina S. Späth, Nicola Mountfort, Doug Kaspar, Henry Dietrich, Daniel R. |
author_sort |
Hitzfeld, Bettina C. |
title |
Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
title_short |
Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
title_full |
Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
title_fullStr |
Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
title_full_unstemmed |
Toxin production in cyanobacterial mats from ponds on the McMurdo Ice Shelf, Antarctica |
title_sort |
toxin production in cyanobacterial mats from ponds on the mcmurdo ice shelf, antarctica |
publishDate |
2000 |
url |
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50339 https://doi.org/10.1016/S0041-0101(00)00103-3 |
long_lat |
ENVELOPE(166.500,166.500,-78.000,-78.000) |
geographic |
McMurdo Ice Shelf |
geographic_facet |
McMurdo Ice Shelf |
genre |
Antarc* Antarctica Ice Shelf McMurdo Ice Shelf |
genre_facet |
Antarc* Antarctica Ice Shelf McMurdo Ice Shelf |
op_source |
Toxicon. 2000, 38(12), pp. 1731-1748. ISSN 0041-0101. Available under: doi:10.1016/S0041-0101(00)00103-3 |
op_relation |
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50339 http://dx.doi.org/10.1016/S0041-0101(00)00103-3 278033180 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/2.0/ |
op_doi |
https://doi.org/10.1016/S0041-0101(00)00103-3 |
container_title |
Toxicon |
container_volume |
38 |
container_issue |
12 |
container_start_page |
1731 |
op_container_end_page |
1748 |
_version_ |
1790594703350038528 |