Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae)
Fragilariopsis cylindrus (Grunow) Willi Krieger, a bipolar psychrophilic and highly abundant diatom, experiences strong shifts in salinity during the formation of sea ice. We investigated the effects of osmotic stress due to an increased salt concentration from 34 to 70 practical salinity units (PSU...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76913 https://doi.org/10.1111/j.1529-8817.2007.00366.x |
id |
ftubkonstanz:oai:kops.uni-konstanz.de:123456789/7715 |
---|---|
record_format |
openpolar |
spelling |
ftubkonstanz:oai:kops.uni-konstanz.de:123456789/7715 2024-02-11T10:08:32+01:00 Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) Krell, Andreas Plettner, Ina John, Uwe Dieckmann, Gerhard Funck, Dietmar 2007 application/pdf http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76913 https://doi.org/10.1111/j.1529-8817.2007.00366.x eng eng http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76913 http://dx.doi.org/10.1111/j.1529-8817.2007.00366.x 304767182 https://rightsstatements.org/page/InC/1.0/ Journal of Phycology. 2007, 43(4), pp. 753-762. ISSN 0022-3646. eISSN 1529-8817. Available under: doi:10.1111/j.1529-8817.2007.00366.x ddc:570 doc-type:article doc-type:Text 2007 ftubkonstanz https://doi.org/10.1111/j.1529-8817.2007.00366.x 2024-01-21T23:52:06Z Fragilariopsis cylindrus (Grunow) Willi Krieger, a bipolar psychrophilic and highly abundant diatom, experiences strong shifts in salinity during the formation of sea ice. We investigated the effects of osmotic stress due to an increased salt concentration from 34 to 70 practical salinity units (PSU) in conjunction with a temperature decrease from 0˚C to -4˚C on the anabolic and catabolic pathways of proline metabolism. Gene expression levels of Δ1-pyrroline-5-carboxylate synthase (P5CS) strongly decreased by a factor of 17.3 in the 70 PSU ⁄ 0˚C treatment, whereas copy numbers of ornithine d-aminotransferase (δ-OAT) increased 7.6-fold. Transcript levels of D1-pyrroline-5-carboxylate reductase (P5CR) and proline dehydrogenase (ProDH) were also slightly up-regulated by 2.5 and 2.88, respectively. This contrasts with findings in higher plants where a reverse regulation of P5CS and δ-OAT was observed and indicates that under elevated external salinities, the ornithine route is preferred to the glutamate pathway in F. cylindrus. Photosynthetic quantum yield at PSII instantly dropped from 0.61 to 0.24 after the upshift in salinity, which confirmed a detrimental effect of elevated salt concentrations on the photosynthetic machinery. Reduced photosynthetic energy capture might explain the preference for the ornithine route over the more energyconsuming proline route. Salt proved to be the dominating stressor, while an additional temperature decrease appeared to have an ameliorating effect. published published Article in Journal/Newspaper Sea ice KOPS - The Institutional Repository of the University of Konstanz Journal of Phycology 43 4 753 762 |
institution |
Open Polar |
collection |
KOPS - The Institutional Repository of the University of Konstanz |
op_collection_id |
ftubkonstanz |
language |
English |
topic |
ddc:570 |
spellingShingle |
ddc:570 Krell, Andreas Plettner, Ina John, Uwe Dieckmann, Gerhard Funck, Dietmar Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
topic_facet |
ddc:570 |
description |
Fragilariopsis cylindrus (Grunow) Willi Krieger, a bipolar psychrophilic and highly abundant diatom, experiences strong shifts in salinity during the formation of sea ice. We investigated the effects of osmotic stress due to an increased salt concentration from 34 to 70 practical salinity units (PSU) in conjunction with a temperature decrease from 0˚C to -4˚C on the anabolic and catabolic pathways of proline metabolism. Gene expression levels of Δ1-pyrroline-5-carboxylate synthase (P5CS) strongly decreased by a factor of 17.3 in the 70 PSU ⁄ 0˚C treatment, whereas copy numbers of ornithine d-aminotransferase (δ-OAT) increased 7.6-fold. Transcript levels of D1-pyrroline-5-carboxylate reductase (P5CR) and proline dehydrogenase (ProDH) were also slightly up-regulated by 2.5 and 2.88, respectively. This contrasts with findings in higher plants where a reverse regulation of P5CS and δ-OAT was observed and indicates that under elevated external salinities, the ornithine route is preferred to the glutamate pathway in F. cylindrus. Photosynthetic quantum yield at PSII instantly dropped from 0.61 to 0.24 after the upshift in salinity, which confirmed a detrimental effect of elevated salt concentrations on the photosynthetic machinery. Reduced photosynthetic energy capture might explain the preference for the ornithine route over the more energyconsuming proline route. Salt proved to be the dominating stressor, while an additional temperature decrease appeared to have an ameliorating effect. published published |
format |
Article in Journal/Newspaper |
author |
Krell, Andreas Plettner, Ina John, Uwe Dieckmann, Gerhard Funck, Dietmar |
author_facet |
Krell, Andreas Plettner, Ina John, Uwe Dieckmann, Gerhard Funck, Dietmar |
author_sort |
Krell, Andreas |
title |
Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
title_short |
Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
title_full |
Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
title_fullStr |
Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
title_full_unstemmed |
Regulation of Proline Metabolism Under Salt Stress in the Psychrophilic Diatom Fragilariopsis Cylindrus (Bacillariophyceae) |
title_sort |
regulation of proline metabolism under salt stress in the psychrophilic diatom fragilariopsis cylindrus (bacillariophyceae) |
publishDate |
2007 |
url |
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76913 https://doi.org/10.1111/j.1529-8817.2007.00366.x |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Journal of Phycology. 2007, 43(4), pp. 753-762. ISSN 0022-3646. eISSN 1529-8817. Available under: doi:10.1111/j.1529-8817.2007.00366.x |
op_relation |
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76913 http://dx.doi.org/10.1111/j.1529-8817.2007.00366.x 304767182 |
op_rights |
https://rightsstatements.org/page/InC/1.0/ |
op_doi |
https://doi.org/10.1111/j.1529-8817.2007.00366.x |
container_title |
Journal of Phycology |
container_volume |
43 |
container_issue |
4 |
container_start_page |
753 |
op_container_end_page |
762 |
_version_ |
1790607919066120192 |