Identification of MicroRNAs in the Coral Stylophora pistillata
Coral reefs are major contributors to marine biodiversity. However, they are in rapid decline due to global environmental changes such as rising sea surface temperatures, ocean acidification, and pollution. Genomic and transcriptomic analyses have broadened our understanding of coral biology, but a...
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ftubkonstanz:oai:kops.uni-konstanz.de:123456789/50922 2024-02-11T10:07:36+01:00 Identification of MicroRNAs in the Coral Stylophora pistillata Liew, Yi Jin Aranda, Manuel Carr, Adrian Baumgarten, Sebastian Zoccola, Didier Tambutté, Sylvie Allemand, Denis Micklem, Gos Voolstra, Christian R. 2014 application/pdf http://nbn-resolving.de/urn:nbn:de:bsz:352-2-14rluey23dz0d3 https://doi.org/10.1371/journal.pone.0091101 eng eng http://nbn-resolving.de/urn:nbn:de:bsz:352-2-14rluey23dz0d3 http://dx.doi.org/10.1371/journal.pone.0091101 24658574 1733492259 http://creativecommons.org/licenses/by/4.0/ PLoS one. Public Library of Science (PLoS). 2014, 9(3), e91101. eISSN 1932-6203. Available under: doi:10.1371/journal.pone.0091101 ddc:570 doc-type:article doc-type:Text 2014 ftubkonstanz https://doi.org/10.1371/journal.pone.0091101 2024-01-21T23:56:47Z Coral reefs are major contributors to marine biodiversity. However, they are in rapid decline due to global environmental changes such as rising sea surface temperatures, ocean acidification, and pollution. Genomic and transcriptomic analyses have broadened our understanding of coral biology, but a study of the microRNA (miRNA) repertoire of corals is missing. miRNAs constitute a class of small non-coding RNAs of ∼22 nt in size that play crucial roles in development, metabolism, and stress response in plants and animals alike. In this study, we examined the coral Stylophora pistillata for the presence of miRNAs and the corresponding core protein machinery required for their processing and function. Based on small RNA sequencing, we present evidence for 31 bona fide microRNAs, 5 of which (miR-100, miR-2022, miR-2023, miR-2030, and miR-2036) are conserved in other metazoans. Homologues of Argonaute, Piwi, Dicer, Drosha, Pasha, and HEN1 were identified in the transcriptome of S. pistillata based on strong sequence conservation with known RNAi proteins, with additional support derived from phylogenetic trees. Examination of putative miRNA gene targets indicates potential roles in development, metabolism, immunity, and biomineralisation for several of the microRNAs. Here, we present first evidence of a functional RNAi machinery and five conserved miRNAs in S. pistillata, implying that miRNAs play a role in organismal biology of scleractinian corals. Analysis of predicted miRNA target genes in S. pistillata suggests potential roles of miRNAs in symbiosis and coral calcification. Given the importance of miRNAs in regulating gene expression in other metazoans, further expression analyses of small non-coding RNAs in transcriptional studies of corals should be informative about miRNA-affected processes and pathways. published published Article in Journal/Newspaper Ocean acidification KOPS - The Institutional Repository of the University of Konstanz PLoS ONE 9 3 e91101 |
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KOPS - The Institutional Repository of the University of Konstanz |
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English |
topic |
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ddc:570 Liew, Yi Jin Aranda, Manuel Carr, Adrian Baumgarten, Sebastian Zoccola, Didier Tambutté, Sylvie Allemand, Denis Micklem, Gos Voolstra, Christian R. Identification of MicroRNAs in the Coral Stylophora pistillata |
topic_facet |
ddc:570 |
description |
Coral reefs are major contributors to marine biodiversity. However, they are in rapid decline due to global environmental changes such as rising sea surface temperatures, ocean acidification, and pollution. Genomic and transcriptomic analyses have broadened our understanding of coral biology, but a study of the microRNA (miRNA) repertoire of corals is missing. miRNAs constitute a class of small non-coding RNAs of ∼22 nt in size that play crucial roles in development, metabolism, and stress response in plants and animals alike. In this study, we examined the coral Stylophora pistillata for the presence of miRNAs and the corresponding core protein machinery required for their processing and function. Based on small RNA sequencing, we present evidence for 31 bona fide microRNAs, 5 of which (miR-100, miR-2022, miR-2023, miR-2030, and miR-2036) are conserved in other metazoans. Homologues of Argonaute, Piwi, Dicer, Drosha, Pasha, and HEN1 were identified in the transcriptome of S. pistillata based on strong sequence conservation with known RNAi proteins, with additional support derived from phylogenetic trees. Examination of putative miRNA gene targets indicates potential roles in development, metabolism, immunity, and biomineralisation for several of the microRNAs. Here, we present first evidence of a functional RNAi machinery and five conserved miRNAs in S. pistillata, implying that miRNAs play a role in organismal biology of scleractinian corals. Analysis of predicted miRNA target genes in S. pistillata suggests potential roles of miRNAs in symbiosis and coral calcification. Given the importance of miRNAs in regulating gene expression in other metazoans, further expression analyses of small non-coding RNAs in transcriptional studies of corals should be informative about miRNA-affected processes and pathways. published published |
format |
Article in Journal/Newspaper |
author |
Liew, Yi Jin Aranda, Manuel Carr, Adrian Baumgarten, Sebastian Zoccola, Didier Tambutté, Sylvie Allemand, Denis Micklem, Gos Voolstra, Christian R. |
author_facet |
Liew, Yi Jin Aranda, Manuel Carr, Adrian Baumgarten, Sebastian Zoccola, Didier Tambutté, Sylvie Allemand, Denis Micklem, Gos Voolstra, Christian R. |
author_sort |
Liew, Yi Jin |
title |
Identification of MicroRNAs in the Coral Stylophora pistillata |
title_short |
Identification of MicroRNAs in the Coral Stylophora pistillata |
title_full |
Identification of MicroRNAs in the Coral Stylophora pistillata |
title_fullStr |
Identification of MicroRNAs in the Coral Stylophora pistillata |
title_full_unstemmed |
Identification of MicroRNAs in the Coral Stylophora pistillata |
title_sort |
identification of micrornas in the coral stylophora pistillata |
publishDate |
2014 |
url |
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-14rluey23dz0d3 https://doi.org/10.1371/journal.pone.0091101 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLoS one. Public Library of Science (PLoS). 2014, 9(3), e91101. eISSN 1932-6203. Available under: doi:10.1371/journal.pone.0091101 |
op_relation |
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-14rluey23dz0d3 http://dx.doi.org/10.1371/journal.pone.0091101 24658574 1733492259 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0091101 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
3 |
container_start_page |
e91101 |
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1790606226332057600 |