Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently eme...
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ftubkonstanz:oai:kops.uni-konstanz.de:123456789/42855 2024-02-11T09:58:53+01:00 Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick 2018 application/pdf http://nbn-resolving.de/urn:nbn:de:bsz:352-2-115a0srsn32rd6 https://doi.org/10.3390/toxins10040147 eng eng http://nbn-resolving.de/urn:nbn:de:bsz:352-2-115a0srsn32rd6 http://dx.doi.org/10.3390/toxins10040147 29614044 507585682 http://creativecommons.org/licenses/by/4.0/ Toxins. 2018, 10(4), 147. eISSN 2072-6651. Available under: doi:10.3390/toxins10040147 arctic benthic mats cyanotoxins ELISA 16S rRNA gene ddc:570 doc-type:article doc-type:Text 2018 ftubkonstanz https://doi.org/10.3390/toxins10040147 2024-01-21T23:53:10Z Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp 3 , ADMAdda 5 , Dhb 7 ] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. published published Article in Journal/Newspaper Antarc* Antarctic Arctic Svalbard KOPS - The Institutional Repository of the University of Konstanz Arctic Antarctic Svalbard Svalbard Archipelago Toxins 10 4 147 |
institution |
Open Polar |
collection |
KOPS - The Institutional Repository of the University of Konstanz |
op_collection_id |
ftubkonstanz |
language |
English |
topic |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene ddc:570 |
spellingShingle |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene ddc:570 Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
topic_facet |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene ddc:570 |
description |
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp 3 , ADMAdda 5 , Dhb 7 ] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. published published |
format |
Article in Journal/Newspaper |
author |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick |
author_facet |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick |
author_sort |
Kleinteich, Julia |
title |
Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_short |
Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full |
Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_fullStr |
Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full_unstemmed |
Toxic Cyanobacteria in Svalbard : Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_sort |
toxic cyanobacteria in svalbard : chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
publishDate |
2018 |
url |
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-115a0srsn32rd6 https://doi.org/10.3390/toxins10040147 |
geographic |
Arctic Antarctic Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Antarctic Svalbard Svalbard Archipelago |
genre |
Antarc* Antarctic Arctic Svalbard |
genre_facet |
Antarc* Antarctic Arctic Svalbard |
op_source |
Toxins. 2018, 10(4), 147. eISSN 2072-6651. Available under: doi:10.3390/toxins10040147 |
op_relation |
http://nbn-resolving.de/urn:nbn:de:bsz:352-2-115a0srsn32rd6 http://dx.doi.org/10.3390/toxins10040147 29614044 507585682 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/toxins10040147 |
container_title |
Toxins |
container_volume |
10 |
container_issue |
4 |
container_start_page |
147 |
_version_ |
1790594703544025088 |