rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean
Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions...
Published in: | European Journal of Phycology |
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ftubkonstanz:oai:kops.uni-konstanz.de:123456789/32868 2024-02-11T10:00:31+01:00 rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Beszteri, Bánk Kroth, Peter G. Valentin, Klaus 2016 application/pdf http://nbn-resolving.de/urn:nbn:de:bsz:352-0-313590 https://doi.org/10.1080/09670262.2015.1077395 eng eng http://nbn-resolving.de/urn:nbn:de:bsz:352-0-313590 http://dx.doi.org/10.1080/09670262.2015.1077395 475368185 http://creativecommons.org/licenses/by-nc-nd/4.0/ European Journal of Phycology. 2016, 51(1), pp. 31-46. ISSN 0967-0262. eISSN 1469-4433. Available under: doi:10.1080/09670262.2015.1077395 ddc:570 doc-type:article doc-type:Text 2016 ftubkonstanz https://doi.org/10.1080/09670262.2015.1077395 2024-01-21T23:57:45Z Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions; as a consequence, summer sea ice cover is decreasing and MYI is disappearing at an alarming rate. Despite its apparent hostility, sea ice is inhabited by a diverse microbial community of bacteria and protists, many of which are photosynthetic. Here we present an assessment of eukaryotic biodiversity in MYI and FYI from the central Arctic Ocean using high-throughput 454 sequencing of 18S rRNA and rDNA amplicons. We compared the rDNA-based ‘total’ biodiversity with the ‘active’ biodiversity from rRNA amplicons and found differences between them including an over-representation of Ciliophora, Bicosoecida and Bacillariophyceae operational taxonomic units (OTUs) in the active part of the community. Differences between the two libraries are more pronounced at the lower taxonomic level: certain genera, such as Melosira, are more abundant in the rRNA library, indicating activity of these genera. Furthermore, we found that one FYI station showed a higher activity of potential grazers which was probably due to the advanced stage of melt evident by higher ice temperatures and highly porous ice compared with the other stations. published published Article in Journal/Newspaper Arctic Arctic Ocean Global warming Sea ice KOPS - The Institutional Repository of the University of Konstanz Arctic Arctic Ocean European Journal of Phycology 51 1 31 46 |
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Open Polar |
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KOPS - The Institutional Repository of the University of Konstanz |
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ftubkonstanz |
language |
English |
topic |
ddc:570 |
spellingShingle |
ddc:570 Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Beszteri, Bánk Kroth, Peter G. Valentin, Klaus rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
topic_facet |
ddc:570 |
description |
Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions; as a consequence, summer sea ice cover is decreasing and MYI is disappearing at an alarming rate. Despite its apparent hostility, sea ice is inhabited by a diverse microbial community of bacteria and protists, many of which are photosynthetic. Here we present an assessment of eukaryotic biodiversity in MYI and FYI from the central Arctic Ocean using high-throughput 454 sequencing of 18S rRNA and rDNA amplicons. We compared the rDNA-based ‘total’ biodiversity with the ‘active’ biodiversity from rRNA amplicons and found differences between them including an over-representation of Ciliophora, Bicosoecida and Bacillariophyceae operational taxonomic units (OTUs) in the active part of the community. Differences between the two libraries are more pronounced at the lower taxonomic level: certain genera, such as Melosira, are more abundant in the rRNA library, indicating activity of these genera. Furthermore, we found that one FYI station showed a higher activity of potential grazers which was probably due to the advanced stage of melt evident by higher ice temperatures and highly porous ice compared with the other stations. published published |
format |
Article in Journal/Newspaper |
author |
Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Beszteri, Bánk Kroth, Peter G. Valentin, Klaus |
author_facet |
Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Beszteri, Bánk Kroth, Peter G. Valentin, Klaus |
author_sort |
Stecher, Anique |
title |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_short |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_full |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_fullStr |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_full_unstemmed |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_sort |
rrna and rdna based assessment of sea ice protist biodiversity from the central arctic ocean |
publishDate |
2016 |
url |
http://nbn-resolving.de/urn:nbn:de:bsz:352-0-313590 https://doi.org/10.1080/09670262.2015.1077395 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Global warming Sea ice |
genre_facet |
Arctic Arctic Ocean Global warming Sea ice |
op_source |
European Journal of Phycology. 2016, 51(1), pp. 31-46. ISSN 0967-0262. eISSN 1469-4433. Available under: doi:10.1080/09670262.2015.1077395 |
op_relation |
http://nbn-resolving.de/urn:nbn:de:bsz:352-0-313590 http://dx.doi.org/10.1080/09670262.2015.1077395 475368185 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1080/09670262.2015.1077395 |
container_title |
European Journal of Phycology |
container_volume |
51 |
container_issue |
1 |
container_start_page |
31 |
op_container_end_page |
46 |
_version_ |
1790596244278607872 |