Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches
Biological soil crusts (BSCs) are key components of polar ecosystems. These complex communities are important for terrestrial polar habitats as they include major primary producers that fix nitrogen, prevent soil erosion and can be regarded as indicators for climate change. To study the genus richne...
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ftubkoeln:oai:USBKOELN.ub.uni-koeln.de:18716 2023-05-15T13:36:41+02:00 Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches Rippin, Martin Borchhardt, Nadine Williams, Laura Colesie, Claudia Jung, Patrick Buedel, Burkhard Karsten, Ulf Becker, Burkhard 2018 https://kups.ub.uni-koeln.de/18716/ eng eng SPRINGER Rippin, Martin orcid:0000-0003-4362-0122 , Borchhardt, Nadine, Williams, Laura, Colesie, Claudia orcid:0000-0003-1136-0290 , Jung, Patrick orcid:0000-0002-7607-3906 , Buedel, Burkhard, Karsten, Ulf and Becker, Burkhard orcid:0000-0002-7965-1396 (2018). Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches. Polar Biol., 41 (5). S. 909 - 924. NEW YORK: SPRINGER. ISSN 1432-2056 ddc:no doc-type:article publishedVersion 2018 ftubkoeln 2022-11-09T07:16:51Z Biological soil crusts (BSCs) are key components of polar ecosystems. These complex communities are important for terrestrial polar habitats as they include major primary producers that fix nitrogen, prevent soil erosion and can be regarded as indicators for climate change. To study the genus richness of microalgae and Cyanobacteria in BSCs, two different methodologies were employed and the outcomes were compared: morphological identification using light microscopy and the annotation of ribosomal sequences taken from metatranscriptomes. The analyzed samples were collected from Ny-Alesund, Svalbard, Norway, and the Juan Carlos I Antarctic Base, Livingston Island, Antarctica. This study focused on the following taxonomic groups: Klebsormidiophyceae, Chlorophyceae, Trebouxiophyceae, Xanthophyceae and Cyanobacteria. In total, combining both approaches, 143 and 103 genera were identified in the Arctic and Antarctic samples, respectively. Furthermore, both techniques concordantly determined 15 taxa in the Arctic and 7 taxa in the Antarctic BSC. In general, the molecular analysis indicated a higher microalgal and cyanobacterial genus richness (about 11 times higher) than the morphological approach. In terms of eukaryotic algae, the two sampling sites displayed comparable genus counts while the cyanobacterial genus richness was much higher in the BSC from Ny-Alesund. For the first time, the presence of the genera Chloroidium, Ankistrodesmus and Dunaliella in polar regions was determined by the metatranscriptomic analysis. Overall, these findings illustrate that only the combination of morphological and molecular techniques, in contrast to one single approach, reveals higher genus richness for complex communities such as polar BSCs. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic Climate change Livingston Island Svalbard Cologne University: KUPS Antarctic Arctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Norway Svalbard The Antarctic |
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Open Polar |
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Cologne University: KUPS |
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ftubkoeln |
language |
English |
topic |
ddc:no |
spellingShingle |
ddc:no Rippin, Martin Borchhardt, Nadine Williams, Laura Colesie, Claudia Jung, Patrick Buedel, Burkhard Karsten, Ulf Becker, Burkhard Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
topic_facet |
ddc:no |
description |
Biological soil crusts (BSCs) are key components of polar ecosystems. These complex communities are important for terrestrial polar habitats as they include major primary producers that fix nitrogen, prevent soil erosion and can be regarded as indicators for climate change. To study the genus richness of microalgae and Cyanobacteria in BSCs, two different methodologies were employed and the outcomes were compared: morphological identification using light microscopy and the annotation of ribosomal sequences taken from metatranscriptomes. The analyzed samples were collected from Ny-Alesund, Svalbard, Norway, and the Juan Carlos I Antarctic Base, Livingston Island, Antarctica. This study focused on the following taxonomic groups: Klebsormidiophyceae, Chlorophyceae, Trebouxiophyceae, Xanthophyceae and Cyanobacteria. In total, combining both approaches, 143 and 103 genera were identified in the Arctic and Antarctic samples, respectively. Furthermore, both techniques concordantly determined 15 taxa in the Arctic and 7 taxa in the Antarctic BSC. In general, the molecular analysis indicated a higher microalgal and cyanobacterial genus richness (about 11 times higher) than the morphological approach. In terms of eukaryotic algae, the two sampling sites displayed comparable genus counts while the cyanobacterial genus richness was much higher in the BSC from Ny-Alesund. For the first time, the presence of the genera Chloroidium, Ankistrodesmus and Dunaliella in polar regions was determined by the metatranscriptomic analysis. Overall, these findings illustrate that only the combination of morphological and molecular techniques, in contrast to one single approach, reveals higher genus richness for complex communities such as polar BSCs. |
format |
Article in Journal/Newspaper |
author |
Rippin, Martin Borchhardt, Nadine Williams, Laura Colesie, Claudia Jung, Patrick Buedel, Burkhard Karsten, Ulf Becker, Burkhard |
author_facet |
Rippin, Martin Borchhardt, Nadine Williams, Laura Colesie, Claudia Jung, Patrick Buedel, Burkhard Karsten, Ulf Becker, Burkhard |
author_sort |
Rippin, Martin |
title |
Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
title_short |
Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
title_full |
Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
title_fullStr |
Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
title_full_unstemmed |
Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches |
title_sort |
genus richness of microalgae and cyanobacteria in biological soil crusts from svalbard and livingston island: morphological versus molecular approaches |
publisher |
SPRINGER |
publishDate |
2018 |
url |
https://kups.ub.uni-koeln.de/18716/ |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Arctic Livingston Island Norway Svalbard The Antarctic |
geographic_facet |
Antarctic Arctic Livingston Island Norway Svalbard The Antarctic |
genre |
Antarc* Antarctic Antarctica Arctic Climate change Livingston Island Svalbard |
genre_facet |
Antarc* Antarctic Antarctica Arctic Climate change Livingston Island Svalbard |
op_relation |
Rippin, Martin orcid:0000-0003-4362-0122 , Borchhardt, Nadine, Williams, Laura, Colesie, Claudia orcid:0000-0003-1136-0290 , Jung, Patrick orcid:0000-0002-7607-3906 , Buedel, Burkhard, Karsten, Ulf and Becker, Burkhard orcid:0000-0002-7965-1396 (2018). Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches. Polar Biol., 41 (5). S. 909 - 924. NEW YORK: SPRINGER. ISSN 1432-2056 |
_version_ |
1766082403430825984 |