Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes
While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetob...
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ftubkoeln:oai:USBKOELN.ub.uni-koeln.de:14140 2023-05-15T13:36:41+02:00 Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes Opazo-Capurro, Andres Higgins, Paul G. Wille, Julia Seifert, Harald Cigarroa, Camila Gonzalez-Munoz, Paulina Quezada-Aguiluz, Mario Dominguez-Yevenes, Mariana Bello-Toledo, Helia Vergara, Luis Gonzalez-Rocha, Gerardo 2019 https://kups.ub.uni-koeln.de/14140/ eng eng FRONTIERS MEDIA SA Opazo-Capurro, Andres orcid:0000-0002-6382-7625 , Higgins, Paul G. orcid:0000-0001-8677-9454 , Wille, Julia, Seifert, Harald, Cigarroa, Camila, Gonzalez-Munoz, Paulina, Quezada-Aguiluz, Mario orcid:0000-0001-9644-8368 , Dominguez-Yevenes, Mariana, Bello-Toledo, Helia, Vergara, Luis and Gonzalez-Rocha, Gerardo (2019). Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes. Front. Cell. Infect. Microbiol., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988 ddc:no doc-type:article publishedVersion 2019 ftubkoeln 2022-11-09T07:14:24Z While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (ICS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic bla(OXA-)(23-like) gene, A154 harbored the plasmid-encoded antibiotic-resistance genes bla(PER-2), tet(B), aph(3')-VIa, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region. Article in Journal/Newspaper Antarc* Antarctic Antarctica Cologne University: KUPS Antarctic Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
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Cologne University: KUPS |
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ftubkoeln |
language |
English |
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spellingShingle |
ddc:no Opazo-Capurro, Andres Higgins, Paul G. Wille, Julia Seifert, Harald Cigarroa, Camila Gonzalez-Munoz, Paulina Quezada-Aguiluz, Mario Dominguez-Yevenes, Mariana Bello-Toledo, Helia Vergara, Luis Gonzalez-Rocha, Gerardo Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
topic_facet |
ddc:no |
description |
While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (ICS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic bla(OXA-)(23-like) gene, A154 harbored the plasmid-encoded antibiotic-resistance genes bla(PER-2), tet(B), aph(3')-VIa, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region. |
format |
Article in Journal/Newspaper |
author |
Opazo-Capurro, Andres Higgins, Paul G. Wille, Julia Seifert, Harald Cigarroa, Camila Gonzalez-Munoz, Paulina Quezada-Aguiluz, Mario Dominguez-Yevenes, Mariana Bello-Toledo, Helia Vergara, Luis Gonzalez-Rocha, Gerardo |
author_facet |
Opazo-Capurro, Andres Higgins, Paul G. Wille, Julia Seifert, Harald Cigarroa, Camila Gonzalez-Munoz, Paulina Quezada-Aguiluz, Mario Dominguez-Yevenes, Mariana Bello-Toledo, Helia Vergara, Luis Gonzalez-Rocha, Gerardo |
author_sort |
Opazo-Capurro, Andres |
title |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_short |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_full |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_fullStr |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_full_unstemmed |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_sort |
genetic features of antarctic acinetobacter radioresistens strain a154 harboring multiple antibiotic-resistance genes |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2019 |
url |
https://kups.ub.uni-koeln.de/14140/ |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
geographic |
Antarctic Fildes Fildes peninsula |
geographic_facet |
Antarctic Fildes Fildes peninsula |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Opazo-Capurro, Andres orcid:0000-0002-6382-7625 , Higgins, Paul G. orcid:0000-0001-8677-9454 , Wille, Julia, Seifert, Harald, Cigarroa, Camila, Gonzalez-Munoz, Paulina, Quezada-Aguiluz, Mario orcid:0000-0001-9644-8368 , Dominguez-Yevenes, Mariana, Bello-Toledo, Helia, Vergara, Luis and Gonzalez-Rocha, Gerardo (2019). Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes. Front. Cell. Infect. Microbiol., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988 |
_version_ |
1766082380530974720 |