Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis
Defects in the biosynthesis and/or deposition of secondary plant cell wall polymers result in the collapse of xylem vessels causing a dwarfed plant stature and an altered plant architecture termed irregular xylem (irx) syndrome. For example, reduced xylan O-acetylation causes strong developmental de...
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ftubkoeln:oai:USBKOELN.ub.uni-koeln.de:13827 2023-05-15T16:06:38+02:00 Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis Ramirez, Vicente Pauly, Markus 2019 https://kups.ub.uni-koeln.de/13827/ eng eng JOHN WILEY & SONS LTD Ramirez, Vicente orcid:0000-0001-9786-497X and Pauly, Markus orcid:0000-0002-3116-2198 (2019). Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis. Plant Direct, 3 (6). CHICHESTER: JOHN WILEY & SONS LTD. ISSN 2475-4455 ddc:no doc-type:article publishedVersion 2019 ftubkoeln 2022-11-09T07:14:14Z Defects in the biosynthesis and/or deposition of secondary plant cell wall polymers result in the collapse of xylem vessels causing a dwarfed plant stature and an altered plant architecture termed irregular xylem (irx) syndrome. For example, reduced xylan O-acetylation causes strong developmental defects and increased freezing tolerance. Recently, we demonstrated that the irx syndrome in the trichome birefringence-like 29/eskimo1 (tbl29/esk1) mutant is dependent on MORE AXILLARY GROWTH 4 (MAX4), a key enzyme in the biosynthesis of the phytohormone strigolactone (SL). In this report, we show that other xylan- and cellulose-deficient secondary wall mutants exhibit increased freezing tolerance correlated with the irx syndrome. In addition, these phenotypes are also dependent on MAX4, suggesting a more general interaction between secondary wall defects and SL biosynthesis. In contrast, MAX4 does not play a role in developmental defects triggered by primary wall deficiencies, suggesting that the interaction is restricted to vascular tissue. Through a reverse genetics approach, the requirement of different components of the SL pathway impacting the irx syndrome in tbl29 was evaluated. Our results show that the tbl29-associated irx phenotypes are dependent on the MAX3 and MAX4 enzymes, involved in the early steps of SL biosynthesis. In contrast, this signaling is independent on downstream enzymes in the biosynthesis and perception of SL such as MAX1 and MAX2. Article in Journal/Newspaper eskimo* Cologne University: KUPS |
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English |
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ddc:no Ramirez, Vicente Pauly, Markus Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
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ddc:no |
description |
Defects in the biosynthesis and/or deposition of secondary plant cell wall polymers result in the collapse of xylem vessels causing a dwarfed plant stature and an altered plant architecture termed irregular xylem (irx) syndrome. For example, reduced xylan O-acetylation causes strong developmental defects and increased freezing tolerance. Recently, we demonstrated that the irx syndrome in the trichome birefringence-like 29/eskimo1 (tbl29/esk1) mutant is dependent on MORE AXILLARY GROWTH 4 (MAX4), a key enzyme in the biosynthesis of the phytohormone strigolactone (SL). In this report, we show that other xylan- and cellulose-deficient secondary wall mutants exhibit increased freezing tolerance correlated with the irx syndrome. In addition, these phenotypes are also dependent on MAX4, suggesting a more general interaction between secondary wall defects and SL biosynthesis. In contrast, MAX4 does not play a role in developmental defects triggered by primary wall deficiencies, suggesting that the interaction is restricted to vascular tissue. Through a reverse genetics approach, the requirement of different components of the SL pathway impacting the irx syndrome in tbl29 was evaluated. Our results show that the tbl29-associated irx phenotypes are dependent on the MAX3 and MAX4 enzymes, involved in the early steps of SL biosynthesis. In contrast, this signaling is independent on downstream enzymes in the biosynthesis and perception of SL such as MAX1 and MAX2. |
format |
Article in Journal/Newspaper |
author |
Ramirez, Vicente Pauly, Markus |
author_facet |
Ramirez, Vicente Pauly, Markus |
author_sort |
Ramirez, Vicente |
title |
Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
title_short |
Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
title_full |
Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
title_fullStr |
Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
title_full_unstemmed |
Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis |
title_sort |
genetic dissection of cell wall defects and the strigolactone pathway in arabidopsis |
publisher |
JOHN WILEY & SONS LTD |
publishDate |
2019 |
url |
https://kups.ub.uni-koeln.de/13827/ |
genre |
eskimo* |
genre_facet |
eskimo* |
op_relation |
Ramirez, Vicente orcid:0000-0001-9786-497X and Pauly, Markus orcid:0000-0002-3116-2198 (2019). Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis. Plant Direct, 3 (6). CHICHESTER: JOHN WILEY & SONS LTD. ISSN 2475-4455 |
_version_ |
1766402624156860416 |