Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set
Barley is worldwide the fourth most important cereal crop and is cultivated in near desert to sub-arctic conditions. The majority of production, around 70 %, is used for animal feed, 20 % are used for malting and the rest for human consumption, with regional differences.Pests and diseases constantly...
Main Author: | |
---|---|
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
Published: |
2023
|
Subjects: | |
Online Access: | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-152128 https://jlupub.ub.uni-giessen.de//handle/jlupub/17403 https://doi.org/10.22029/jlupub-16781 |
id |
ftubgiessen:oai:jlupub.ub.uni-giessen.de:jlupub/17403 |
---|---|
record_format |
openpolar |
spelling |
ftubgiessen:oai:jlupub.ub.uni-giessen.de:jlupub/17403 2024-05-12T08:00:41+00:00 Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set Identifizierung von QTL zur Resistenz gegen zwei Pilzpathogene, Pyrenophora teres f. teres und Bipolaris sorokiniana, in einem Gerstendiversitätsset (Hordeum vulgare L.) Novakazi, Fluturë 2023-06-12T08:03:41Z application/pdf http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-152128 https://jlupub.ub.uni-giessen.de//handle/jlupub/17403 https://doi.org/10.22029/jlupub-16781 en eng 978-3-95547-097-5 http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-152128 https://jlupub.ub.uni-giessen.de//handle/jlupub/17403 http://dx.doi.org/10.22029/jlupub-16781 In Copyright http://rightsstatements.org/page/InC/1.0/ barley GWAS net blotch spot blotch ddc:630 doctoralThesis 2023 ftubgiessen https://doi.org/10.22029/jlupub-16781 2024-04-17T09:56:48Z Barley is worldwide the fourth most important cereal crop and is cultivated in near desert to sub-arctic conditions. The majority of production, around 70 %, is used for animal feed, 20 % are used for malting and the rest for human consumption, with regional differences.Pests and diseases constantly result in high yield losses. Two worldwide important fungal foliar diseases of barley are Pyrenophora teres f. teres (Ptt) and Bipolaris sorokiniana (Bs), the causal agents of net blotch and spot blotch, respectively. Yield losses are on average around 40 % and can amount to over 70 % in years with epidemics. Both pathogens are highly variable and the occurrence of new pathogenic strains demands for breeding of resistant cultivars.In order to identify new resistance sources, a diverse barley set comprising 449 accessions originating from over 50 different regions all over the world, expressing different levels of resistance against both pathogens, was screened. Seedling resistance was tested under controlled greenhouse conditions with three isolates of each pathogen. Adult plant resistance was tested in field trials at three and two locations for Ptt and Bs, respectively. Phenotypic results showed a wide range of the level of resistance and significant differences between accessions were observed in all trials. The set was genotyped using the Illumina iSelect 50k barley SNP chip. After filtering for quality control parameters, i.e. failure rates < 10%, heterozygous calls < 12.5% and minor allele frequency > 5%, 33,318 polymorphic, mapped SNPs were left for further genome-wide association studies (GWAS). Markers were mapped against the barley reference sequence. GWAS was conducted using a compressed mixed linear model (CMLM) including population structure and kinship matrix. GWAS for Ptt revealed 254 significant marker-trait associations (MTAs) located on chromosomes 3H, 4H, 5H, 6H, and 7H and corresponding to 15 quantitative trait loci (QTL). Four of these loci are putatively new and were not previously ... Doctoral or Postdoctoral Thesis Arctic Publication Server of the Justus-Liebig-University of Giessen Arctic |
institution |
Open Polar |
collection |
Publication Server of the Justus-Liebig-University of Giessen |
op_collection_id |
ftubgiessen |
language |
English |
topic |
barley GWAS net blotch spot blotch ddc:630 |
spellingShingle |
barley GWAS net blotch spot blotch ddc:630 Novakazi, Fluturë Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
topic_facet |
barley GWAS net blotch spot blotch ddc:630 |
description |
Barley is worldwide the fourth most important cereal crop and is cultivated in near desert to sub-arctic conditions. The majority of production, around 70 %, is used for animal feed, 20 % are used for malting and the rest for human consumption, with regional differences.Pests and diseases constantly result in high yield losses. Two worldwide important fungal foliar diseases of barley are Pyrenophora teres f. teres (Ptt) and Bipolaris sorokiniana (Bs), the causal agents of net blotch and spot blotch, respectively. Yield losses are on average around 40 % and can amount to over 70 % in years with epidemics. Both pathogens are highly variable and the occurrence of new pathogenic strains demands for breeding of resistant cultivars.In order to identify new resistance sources, a diverse barley set comprising 449 accessions originating from over 50 different regions all over the world, expressing different levels of resistance against both pathogens, was screened. Seedling resistance was tested under controlled greenhouse conditions with three isolates of each pathogen. Adult plant resistance was tested in field trials at three and two locations for Ptt and Bs, respectively. Phenotypic results showed a wide range of the level of resistance and significant differences between accessions were observed in all trials. The set was genotyped using the Illumina iSelect 50k barley SNP chip. After filtering for quality control parameters, i.e. failure rates < 10%, heterozygous calls < 12.5% and minor allele frequency > 5%, 33,318 polymorphic, mapped SNPs were left for further genome-wide association studies (GWAS). Markers were mapped against the barley reference sequence. GWAS was conducted using a compressed mixed linear model (CMLM) including population structure and kinship matrix. GWAS for Ptt revealed 254 significant marker-trait associations (MTAs) located on chromosomes 3H, 4H, 5H, 6H, and 7H and corresponding to 15 quantitative trait loci (QTL). Four of these loci are putatively new and were not previously ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Novakazi, Fluturë |
author_facet |
Novakazi, Fluturë |
author_sort |
Novakazi, Fluturë |
title |
Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
title_short |
Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
title_full |
Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
title_fullStr |
Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
title_full_unstemmed |
Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set |
title_sort |
identification of qtl for resistance against two fungal pathogens, pyrenophora teres f. teres and bipolaris sorokiniana, in a barley (hordeum vulgare l.) diversity set |
publishDate |
2023 |
url |
http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-152128 https://jlupub.ub.uni-giessen.de//handle/jlupub/17403 https://doi.org/10.22029/jlupub-16781 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
978-3-95547-097-5 http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-152128 https://jlupub.ub.uni-giessen.de//handle/jlupub/17403 http://dx.doi.org/10.22029/jlupub-16781 |
op_rights |
In Copyright http://rightsstatements.org/page/InC/1.0/ |
op_doi |
https://doi.org/10.22029/jlupub-16781 |
_version_ |
1798842656529317888 |