Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds
Moodley Y, Masello J, Cole TL, et al. Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds. Molecular Ecology Resources . 2015;15(5):1046-1058. Microsatellite loci are ideal for testing hypotheses relating to genetic segregation at fine spati...
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ftubbiepub:oai:pub.uni-bielefeld.de:2984182 2023-12-10T09:41:46+01:00 Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds Moodley, Yoshan Masello, Juan Cole, Theresa L. Calderon, Luciano Munimanda, Gopi K. Thali, Marco R. Alderman, Rachael Cuthbert, Richard J. Marin, Manuel Massaro, Melanie Navarro, Joan Phillips, Richard A. Ryan, Peter G. Suazo, Cristián G. Cherel, Yves Weimerskirch, Henri Quillfeldt, Petra 2015 https://pub.uni-bielefeld.de/record/2984182 eng eng Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.12372 info:eu-repo/semantics/altIdentifier/issn/1755-098X info:eu-repo/semantics/altIdentifier/issn/1755-0998 https://pub.uni-bielefeld.de/record/2984182 info:eu-repo/semantics/closedAccess http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article doc-type:article text 2015 ftubbiepub https://doi.org/10.1111/1755-0998.12372 2023-11-13T00:05:37Z Moodley Y, Masello J, Cole TL, et al. Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds. Molecular Ecology Resources . 2015;15(5):1046-1058. Microsatellite loci are ideal for testing hypotheses relating to genetic segregation at fine spatio-temporal scales. They are also conserved among closely related species, making them potentially useful for clarifying interspecific relationships between recently diverged taxa. However, mutations at primer binding sites may lead to increased nonamplification, or disruptions that may result in decreased polymorphism in nontarget species. Furthermore, high mutation rates and constraints on allele size may also with evolutionary time, promote an increase in convergently evolved allele size classes, biasing measures of interspecific genetic differentiation. Here, we used next-generation sequencing to develop microsatellite markers from a shotgun genome sequence of the sub-Antarctic seabird, the thin-billed prion (Pachyptila belcheri), that we tested for cross-species amplification in other Pachyptila and related sub-Antarctic species. We found that heterozygosity decreased and the proportion of nonamplifying loci increased with phylogenetic distance from the target species. Surprisingly, we found that species trees estimated from interspecific FST provided better approximations of mtDNA relationships among the studied species than those estimated using DC, even though FST was more affected by null alleles. We observed a significantly nonlinear second order polynomial relationship between microsatellite and mtDNA distances. We propose that the loss of linearity with increasing mtDNA distance stems from an increasing proportion of homoplastic allele size classes that are identical in state, but not identical by descent. Therefore, despite high cross-species amplification success and high polymorphism among the closely related Pachyptila species, we caution against the use of microsatellites in phylogenetic inference ... Article in Journal/Newspaper Antarc* Antarctic PUB - Publications at Bielefeld University Antarctic Molecular Ecology Resources 15 5 1046 1058 |
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Open Polar |
collection |
PUB - Publications at Bielefeld University |
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ftubbiepub |
language |
English |
description |
Moodley Y, Masello J, Cole TL, et al. Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds. Molecular Ecology Resources . 2015;15(5):1046-1058. Microsatellite loci are ideal for testing hypotheses relating to genetic segregation at fine spatio-temporal scales. They are also conserved among closely related species, making them potentially useful for clarifying interspecific relationships between recently diverged taxa. However, mutations at primer binding sites may lead to increased nonamplification, or disruptions that may result in decreased polymorphism in nontarget species. Furthermore, high mutation rates and constraints on allele size may also with evolutionary time, promote an increase in convergently evolved allele size classes, biasing measures of interspecific genetic differentiation. Here, we used next-generation sequencing to develop microsatellite markers from a shotgun genome sequence of the sub-Antarctic seabird, the thin-billed prion (Pachyptila belcheri), that we tested for cross-species amplification in other Pachyptila and related sub-Antarctic species. We found that heterozygosity decreased and the proportion of nonamplifying loci increased with phylogenetic distance from the target species. Surprisingly, we found that species trees estimated from interspecific FST provided better approximations of mtDNA relationships among the studied species than those estimated using DC, even though FST was more affected by null alleles. We observed a significantly nonlinear second order polynomial relationship between microsatellite and mtDNA distances. We propose that the loss of linearity with increasing mtDNA distance stems from an increasing proportion of homoplastic allele size classes that are identical in state, but not identical by descent. Therefore, despite high cross-species amplification success and high polymorphism among the closely related Pachyptila species, we caution against the use of microsatellites in phylogenetic inference ... |
format |
Article in Journal/Newspaper |
author |
Moodley, Yoshan Masello, Juan Cole, Theresa L. Calderon, Luciano Munimanda, Gopi K. Thali, Marco R. Alderman, Rachael Cuthbert, Richard J. Marin, Manuel Massaro, Melanie Navarro, Joan Phillips, Richard A. Ryan, Peter G. Suazo, Cristián G. Cherel, Yves Weimerskirch, Henri Quillfeldt, Petra |
spellingShingle |
Moodley, Yoshan Masello, Juan Cole, Theresa L. Calderon, Luciano Munimanda, Gopi K. Thali, Marco R. Alderman, Rachael Cuthbert, Richard J. Marin, Manuel Massaro, Melanie Navarro, Joan Phillips, Richard A. Ryan, Peter G. Suazo, Cristián G. Cherel, Yves Weimerskirch, Henri Quillfeldt, Petra Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
author_facet |
Moodley, Yoshan Masello, Juan Cole, Theresa L. Calderon, Luciano Munimanda, Gopi K. Thali, Marco R. Alderman, Rachael Cuthbert, Richard J. Marin, Manuel Massaro, Melanie Navarro, Joan Phillips, Richard A. Ryan, Peter G. Suazo, Cristián G. Cherel, Yves Weimerskirch, Henri Quillfeldt, Petra |
author_sort |
Moodley, Yoshan |
title |
Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
title_short |
Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
title_full |
Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
title_fullStr |
Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
title_full_unstemmed |
Evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
title_sort |
evolutionary factors affecting the cross‐species utility of newly developed microsatellite markers in seabirds |
publisher |
Wiley |
publishDate |
2015 |
url |
https://pub.uni-bielefeld.de/record/2984182 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.12372 info:eu-repo/semantics/altIdentifier/issn/1755-098X info:eu-repo/semantics/altIdentifier/issn/1755-0998 https://pub.uni-bielefeld.de/record/2984182 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/1755-0998.12372 |
container_title |
Molecular Ecology Resources |
container_volume |
15 |
container_issue |
5 |
container_start_page |
1046 |
op_container_end_page |
1058 |
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1784902540627804160 |