A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains
Hansen FT, Madsen CK, Nordland AM, Grasser M, Merkle T, Grasser KD. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry . 2008;47(50):13207-13214. The A/T-rich interaction domain (ARID) and the HMG-box domain represent DNA-interaction mo...
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ftubbiepub:oai:pub.uni-bielefeld.de:1636583 2023-05-15T17:24:40+02:00 A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains Hansen, Frederik T. Madsen, Claus K. Nordland, Anne Mette Grasser, Marion Merkle, Thomas Grasser, Klaus D. 2008 https://pub.uni-bielefeld.de/record/1636583 eng eng AMER CHEMICAL SOC info:eu-repo/semantics/altIdentifier/doi/10.1021/bi801772k info:eu-repo/semantics/altIdentifier/issn/0006-2960 info:eu-repo/semantics/altIdentifier/issn/1520-4995 info:eu-repo/semantics/altIdentifier/wos/000261510500009 info:eu-repo/semantics/altIdentifier/pmid/19053246 https://pub.uni-bielefeld.de/record/1636583 info:eu-repo/semantics/closedAccess http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article doc-type:article text 2008 ftubbiepub https://doi.org/10.1021/bi801772k 2022-02-08T22:29:13Z Hansen FT, Madsen CK, Nordland AM, Grasser M, Merkle T, Grasser KD. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry . 2008;47(50):13207-13214. The A/T-rich interaction domain (ARID) and the HMG-box domain represent DNA-interaction modules that are found in sequence-specific as well as nonsequence-specific DNA-binding proteins. Both domains are found in a variety of DNA-interacting proteins in a wide range of eukaryotic organisms. Proteins that contain both an ARID and an HMG-box domain, here termed ARID-HMG proteins, appear to be specific for plants. This protein family is conserved in higher plants (both mono- and dicot plants) as well as lower plants such as the moss Physcomitrella. Since ARID-HMG proteins have not been studied experimentally, we have examined here two family members from Arabidopsis. The genes encoding ARID-HMG1 and ARID-HMG2 are widely expressed in Arabidopsis but at different levels. Subcellular localization experiments studying ARID-HMG1 and ARID-HMG2 fused to GFP by fluorescence microscopy show that both proteins localize primarily to cell nuclei. Analyses of the DNA-binding properties using electrophoretic mobility shift assays revealed that mediated by the HMG-box domain, ARID-HMG I binds structure specifically to DNA minicircles. Mediated by the ARID, the protein binds preferentially to A/T-rich DNA, when compared with G/C-rich DNA. Therefore, both DNA-binding domains contribute to the DNA interactions of ARID-HMGI. Accordingly, the protein combines DNA-binding properties characteristic of ARID and HMG-box proteins. Article in Journal/Newspaper Nordland Nordland Nordland PUB - Publications at Bielefeld University Biochemistry 47 50 13207 13214 |
institution |
Open Polar |
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PUB - Publications at Bielefeld University |
op_collection_id |
ftubbiepub |
language |
English |
description |
Hansen FT, Madsen CK, Nordland AM, Grasser M, Merkle T, Grasser KD. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry . 2008;47(50):13207-13214. The A/T-rich interaction domain (ARID) and the HMG-box domain represent DNA-interaction modules that are found in sequence-specific as well as nonsequence-specific DNA-binding proteins. Both domains are found in a variety of DNA-interacting proteins in a wide range of eukaryotic organisms. Proteins that contain both an ARID and an HMG-box domain, here termed ARID-HMG proteins, appear to be specific for plants. This protein family is conserved in higher plants (both mono- and dicot plants) as well as lower plants such as the moss Physcomitrella. Since ARID-HMG proteins have not been studied experimentally, we have examined here two family members from Arabidopsis. The genes encoding ARID-HMG1 and ARID-HMG2 are widely expressed in Arabidopsis but at different levels. Subcellular localization experiments studying ARID-HMG1 and ARID-HMG2 fused to GFP by fluorescence microscopy show that both proteins localize primarily to cell nuclei. Analyses of the DNA-binding properties using electrophoretic mobility shift assays revealed that mediated by the HMG-box domain, ARID-HMG I binds structure specifically to DNA minicircles. Mediated by the ARID, the protein binds preferentially to A/T-rich DNA, when compared with G/C-rich DNA. Therefore, both DNA-binding domains contribute to the DNA interactions of ARID-HMGI. Accordingly, the protein combines DNA-binding properties characteristic of ARID and HMG-box proteins. |
format |
Article in Journal/Newspaper |
author |
Hansen, Frederik T. Madsen, Claus K. Nordland, Anne Mette Grasser, Marion Merkle, Thomas Grasser, Klaus D. |
spellingShingle |
Hansen, Frederik T. Madsen, Claus K. Nordland, Anne Mette Grasser, Marion Merkle, Thomas Grasser, Klaus D. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
author_facet |
Hansen, Frederik T. Madsen, Claus K. Nordland, Anne Mette Grasser, Marion Merkle, Thomas Grasser, Klaus D. |
author_sort |
Hansen, Frederik T. |
title |
A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
title_short |
A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
title_full |
A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
title_fullStr |
A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
title_full_unstemmed |
A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains |
title_sort |
novel family of plant dna-binding proteins containing both hmg-box and at-rich interaction domains |
publisher |
AMER CHEMICAL SOC |
publishDate |
2008 |
url |
https://pub.uni-bielefeld.de/record/1636583 |
genre |
Nordland Nordland Nordland |
genre_facet |
Nordland Nordland Nordland |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1021/bi801772k info:eu-repo/semantics/altIdentifier/issn/0006-2960 info:eu-repo/semantics/altIdentifier/issn/1520-4995 info:eu-repo/semantics/altIdentifier/wos/000261510500009 info:eu-repo/semantics/altIdentifier/pmid/19053246 https://pub.uni-bielefeld.de/record/1636583 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1021/bi801772k |
container_title |
Biochemistry |
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47 |
container_issue |
50 |
container_start_page |
13207 |
op_container_end_page |
13214 |
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1766115784713568256 |