A combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (Scophtalmus maximus)

Background Genomic resources for plant and animal species that are under exploitation primarily for human consumption are increasingly important, among other things, for understanding physiological processes and for establishing adequate genetic selection programs. Current available techniques for h...

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Bibliographic Details
Published in:BMC Genomics
Main Authors: Ribas, Laia, Gómez-Pardo, Belén, Fernández, Carlos, Alvarez-Dios, José Antonio, Gómez-Tato, Antonio, Quiroga, María Isabel, Planas Vilarnau, Josep, Sitjà-Bobadilla, Ariadna, Martínez, Paulino, Piferrer, Francesc
Format: Article in Journal/Newspaper
Language:English
Published: BioMed Central 2013
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Online Access:http://hdl.handle.net/2445/69394
Description
Summary:Background Genomic resources for plant and animal species that are under exploitation primarily for human consumption are increasingly important, among other things, for understanding physiological processes and for establishing adequate genetic selection programs. Current available techniques for high-throughput sequencing have been implemented in a number of species, including fish, to obtain a proper description of the transcriptome. The objective of this study was to generate a comprehensive transcriptomic database in turbot, a highly priced farmed fish species in Europe, with potential expansion to other areas of the world, for which there are unsolved production bottlenecks, to understand better reproductive- and immune-related functions. This information is essential to implement marker assisted selection programs useful for the turbot industry [.].