Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters
The sibling species Anisakis simplex (s.s.) and Anisakis pegreffii are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fou...
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ftubarcepubl:oai:diposit.ub.edu:2445/179917 2024-02-11T10:06:53+01:00 Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters Roca Geronès, Xavier Alcover Amengual, Maria Magdalena Godínez González, Carla Montoliu Sanllehy, Isabel de Fisa Saladrigas, Roser 2021-08-21 application/pdf http://hdl.handle.net/2445/179917 eng eng MDPI Reproducció del document publicat a: https://doi.org/10.3390/ani11082458 Animals, 2021 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.3390/ani11082458 2076-2615 http://hdl.handle.net/2445/179917 713700 34438915 cc-by (c) Roca Geronès, Xavier et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Anisakiosi Nematodes Parasitologia Seguretat alimentària Anisakiasis Parasitology Food security info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftubarcepubl https://doi.org/10.3390/ani11082458 2024-01-24T01:15:53Z The sibling species Anisakis simplex (s.s.) and Anisakis pegreffii are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fourth-stage and adult forms. The aim of this study was to confirm the presence of hybrid genotypes in larvae parasitizing fish caught in sympatric and allopatric Spanish marine waters, the North-East Atlantic and West Mediterranean, respectively, and to study possible differences in the growth behaviour between genotypes. Of the 254 molecularly analysed larvae, 18 were identified as hybrids by PCR-RFLP analysis of the rDNA ITS region, 11 of which were subsequently confirmed by EF1 α-1 nDNA gene sequencing. These results therefore indicate an overestimation of hybrid genotypes when identification is based only on the ITS region. We also report the detection of a hybrid specimen in a host from the West Mediterranean, considered an allopatric zone. Additionally, fourth-stage larvae with a hybrid genotype were obtained in vitro for the first time, and no differences were observed in their growth behaviour compared to larvae with A. simplex (s.s.) and A. pegreffii genotypes. Article in Journal/Newspaper North East Atlantic Dipòsit Digital de la Universitat de Barcelona Animals 11 8 2458 |
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Open Polar |
collection |
Dipòsit Digital de la Universitat de Barcelona |
op_collection_id |
ftubarcepubl |
language |
English |
topic |
Anisakiosi Nematodes Parasitologia Seguretat alimentària Anisakiasis Parasitology Food security |
spellingShingle |
Anisakiosi Nematodes Parasitologia Seguretat alimentària Anisakiasis Parasitology Food security Roca Geronès, Xavier Alcover Amengual, Maria Magdalena Godínez González, Carla Montoliu Sanllehy, Isabel de Fisa Saladrigas, Roser Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
topic_facet |
Anisakiosi Nematodes Parasitologia Seguretat alimentària Anisakiasis Parasitology Food security |
description |
The sibling species Anisakis simplex (s.s.) and Anisakis pegreffii are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fourth-stage and adult forms. The aim of this study was to confirm the presence of hybrid genotypes in larvae parasitizing fish caught in sympatric and allopatric Spanish marine waters, the North-East Atlantic and West Mediterranean, respectively, and to study possible differences in the growth behaviour between genotypes. Of the 254 molecularly analysed larvae, 18 were identified as hybrids by PCR-RFLP analysis of the rDNA ITS region, 11 of which were subsequently confirmed by EF1 α-1 nDNA gene sequencing. These results therefore indicate an overestimation of hybrid genotypes when identification is based only on the ITS region. We also report the detection of a hybrid specimen in a host from the West Mediterranean, considered an allopatric zone. Additionally, fourth-stage larvae with a hybrid genotype were obtained in vitro for the first time, and no differences were observed in their growth behaviour compared to larvae with A. simplex (s.s.) and A. pegreffii genotypes. |
format |
Article in Journal/Newspaper |
author |
Roca Geronès, Xavier Alcover Amengual, Maria Magdalena Godínez González, Carla Montoliu Sanllehy, Isabel de Fisa Saladrigas, Roser |
author_facet |
Roca Geronès, Xavier Alcover Amengual, Maria Magdalena Godínez González, Carla Montoliu Sanllehy, Isabel de Fisa Saladrigas, Roser |
author_sort |
Roca Geronès, Xavier |
title |
Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
title_short |
Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
title_full |
Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
title_fullStr |
Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
title_full_unstemmed |
Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
title_sort |
hybrid genotype of anisakis simplex (s.s.) and a. pegreffii identified in third- and fourth-stage larvae from sympatric and allopatric spanish marine waters |
publisher |
MDPI |
publishDate |
2021 |
url |
http://hdl.handle.net/2445/179917 |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_relation |
Reproducció del document publicat a: https://doi.org/10.3390/ani11082458 Animals, 2021 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.3390/ani11082458 2076-2615 http://hdl.handle.net/2445/179917 713700 34438915 |
op_rights |
cc-by (c) Roca Geronès, Xavier et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/ani11082458 |
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Animals |
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11 |
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2458 |
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1790604902845644800 |