Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria
Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial behavior a...
Published in: | Microbial Ecology |
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Language: | English |
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Springer Verlag
2020
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ftubarcepubl:oai:diposit.ub.edu:2445/174782 2024-02-11T09:58:17+01:00 Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria Baeza, Nicolás Mercadé Gil, M. Elena 2020-10-06 12 p. application/pdf http://hdl.handle.net/2445/174782 eng eng Springer Verlag Reproducció del document publicat a: https://doi.org/10.1007/s00248-020-01614-6 Microbial Ecology, 2020, vol. 2020 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1007/s00248-020-01614-6 0095-3628 http://hdl.handle.net/2445/174782 706491 33025062 (c) cc-by, Baeza et. al., 2020 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Vesícula biliar Trifosfat d'adenosina Antàrtida Supervivència en indrets salvatges Bacteris Gallbladder Adenosine triphospahatase Antarctica Wilderness survival Bacteria info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/publishedVersion 2020 ftubarcepubl https://doi.org/10.1007/s00248-020-01614-6 2024-01-24T01:14:23Z Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial behavior and control biofilm formation when necessary. We used a collection of cold-adapted Antarctic Gram-negative bacteria to study whether their ability to form biofilms is associated with a capacity to produce membrane vesicles and secrete extracellular ATP. In most of the studied strains, no correlation was found between biofilm formation and these two factors. Only Shewanella vesiculosa M7T secreted high levels of extracellular ATP, and its membrane vesicles caused a significant increase in the speed and amount of biofilm formation. In this strain, an important portion of the exogenous ATP was contained in membrane vesicles, where it was protected from apyrase treatment. These results confirm that ATP influences biofilm formation. Although the role of extracellular ATP in prokaryotes is still not well understood, the metabolic cost of its production suggests it has an important function, such as a role in biofilm formation. Thus, the liberation of extracellular ATP through membrane vesicles and its function deserve further study. Article in Journal/Newspaper Antarc* Antarctic Antarctica Dipòsit Digital de la Universitat de Barcelona Antarctic Microbial Ecology 81 3 645 656 |
institution |
Open Polar |
collection |
Dipòsit Digital de la Universitat de Barcelona |
op_collection_id |
ftubarcepubl |
language |
English |
topic |
Vesícula biliar Trifosfat d'adenosina Antàrtida Supervivència en indrets salvatges Bacteris Gallbladder Adenosine triphospahatase Antarctica Wilderness survival Bacteria |
spellingShingle |
Vesícula biliar Trifosfat d'adenosina Antàrtida Supervivència en indrets salvatges Bacteris Gallbladder Adenosine triphospahatase Antarctica Wilderness survival Bacteria Baeza, Nicolás Mercadé Gil, M. Elena Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
topic_facet |
Vesícula biliar Trifosfat d'adenosina Antàrtida Supervivència en indrets salvatges Bacteris Gallbladder Adenosine triphospahatase Antarctica Wilderness survival Bacteria |
description |
Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial behavior and control biofilm formation when necessary. We used a collection of cold-adapted Antarctic Gram-negative bacteria to study whether their ability to form biofilms is associated with a capacity to produce membrane vesicles and secrete extracellular ATP. In most of the studied strains, no correlation was found between biofilm formation and these two factors. Only Shewanella vesiculosa M7T secreted high levels of extracellular ATP, and its membrane vesicles caused a significant increase in the speed and amount of biofilm formation. In this strain, an important portion of the exogenous ATP was contained in membrane vesicles, where it was protected from apyrase treatment. These results confirm that ATP influences biofilm formation. Although the role of extracellular ATP in prokaryotes is still not well understood, the metabolic cost of its production suggests it has an important function, such as a role in biofilm formation. Thus, the liberation of extracellular ATP through membrane vesicles and its function deserve further study. |
format |
Article in Journal/Newspaper |
author |
Baeza, Nicolás Mercadé Gil, M. Elena |
author_facet |
Baeza, Nicolás Mercadé Gil, M. Elena |
author_sort |
Baeza, Nicolás |
title |
Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
title_short |
Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
title_full |
Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
title_fullStr |
Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
title_full_unstemmed |
Relationship between membrane vesicles, extracellular ATP and biofilm formation in Antarctic Gram-negative bacteria |
title_sort |
relationship between membrane vesicles, extracellular atp and biofilm formation in antarctic gram-negative bacteria |
publisher |
Springer Verlag |
publishDate |
2020 |
url |
http://hdl.handle.net/2445/174782 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Reproducció del document publicat a: https://doi.org/10.1007/s00248-020-01614-6 Microbial Ecology, 2020, vol. 2020 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1007/s00248-020-01614-6 0095-3628 http://hdl.handle.net/2445/174782 706491 33025062 |
op_rights |
(c) cc-by, Baeza et. al., 2020 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00248-020-01614-6 |
container_title |
Microbial Ecology |
container_volume |
81 |
container_issue |
3 |
container_start_page |
645 |
op_container_end_page |
656 |
_version_ |
1790593905706663936 |