The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography
In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling followed by freeze substitution (FS) and sect...
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ftubarcepubl:oai:diposit.ub.edu:2445/127822 2024-02-11T09:57:34+01:00 The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography Delgado, Lídia Carrión, Ornella Martínez, Gema López Iglesias, Carmen Mercadé Gil, M. Elena 2013-09-09 1 p. application/pdf http://hdl.handle.net/2445/127822 eng eng Public Library of Science (PLoS) Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0073297 PLoS One, 2013, vol. 8, num. 9, p. e73297- Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1371/journal.pone.0073297 1932-6203 http://hdl.handle.net/2445/127822 628227 24039905 cc-by (c) Delgado, Lídia et al., 2013 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Biologia Biotecnologia Bacteriologia Anàlisi instrumental Microbiologia Biology Biotechnology Bacteriology Instrumental analysis Microbiology info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftubarcepubl https://doi.org/10.1371/journal.pone.0073297 2024-01-24T01:05:29Z In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling followed by freeze substitution (FS) and sectioning, freeze fracture (FF) and observation of replica, or cryoelectron microscopy of vitreous sections (CEMOVIS) now allow visualization of biological samples close to their native state, enabling us to refine our knowledge of already known bacterial structures and to discover new ones. Application of these techniques to the new Antarctic cold-adapted bacterium Pseudomonas deceptionensis M1T has demonstrated the existence of a previously undescribed cytoplasmic structure that does not correspond to known bacterial inclusion bodies or membranous formations. This structure, which we term a "stack", was mainly visualized in slow growing cultures of P. deceptionensis M1T and can be described as a set of stacked membranous discs usually arranged perpendicularly to the cell membrane, but not continuous with it, and found in variable number in different locations within the cell. Regardless of their position, stacks were mostly observed very close to DNA fibers. Stacks are not exclusive to P. deceptionensis M1T and were also visualized in slow-growing cultures of other bacteria. This new structure deserves further study using cryoelectron tomography to refine its configuration and to establish whether its function could be related to chromosome dynamics. Article in Journal/Newspaper Antarc* Antarctic Dipòsit Digital de la Universitat de Barcelona Antarctic The Antarctic PLoS ONE 8 9 e73297 |
institution |
Open Polar |
collection |
Dipòsit Digital de la Universitat de Barcelona |
op_collection_id |
ftubarcepubl |
language |
English |
topic |
Biologia Biotecnologia Bacteriologia Anàlisi instrumental Microbiologia Biology Biotechnology Bacteriology Instrumental analysis Microbiology |
spellingShingle |
Biologia Biotecnologia Bacteriologia Anàlisi instrumental Microbiologia Biology Biotechnology Bacteriology Instrumental analysis Microbiology Delgado, Lídia Carrión, Ornella Martínez, Gema López Iglesias, Carmen Mercadé Gil, M. Elena The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
topic_facet |
Biologia Biotecnologia Bacteriologia Anàlisi instrumental Microbiologia Biology Biotechnology Bacteriology Instrumental analysis Microbiology |
description |
In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling followed by freeze substitution (FS) and sectioning, freeze fracture (FF) and observation of replica, or cryoelectron microscopy of vitreous sections (CEMOVIS) now allow visualization of biological samples close to their native state, enabling us to refine our knowledge of already known bacterial structures and to discover new ones. Application of these techniques to the new Antarctic cold-adapted bacterium Pseudomonas deceptionensis M1T has demonstrated the existence of a previously undescribed cytoplasmic structure that does not correspond to known bacterial inclusion bodies or membranous formations. This structure, which we term a "stack", was mainly visualized in slow growing cultures of P. deceptionensis M1T and can be described as a set of stacked membranous discs usually arranged perpendicularly to the cell membrane, but not continuous with it, and found in variable number in different locations within the cell. Regardless of their position, stacks were mostly observed very close to DNA fibers. Stacks are not exclusive to P. deceptionensis M1T and were also visualized in slow-growing cultures of other bacteria. This new structure deserves further study using cryoelectron tomography to refine its configuration and to establish whether its function could be related to chromosome dynamics. |
format |
Article in Journal/Newspaper |
author |
Delgado, Lídia Carrión, Ornella Martínez, Gema López Iglesias, Carmen Mercadé Gil, M. Elena |
author_facet |
Delgado, Lídia Carrión, Ornella Martínez, Gema López Iglesias, Carmen Mercadé Gil, M. Elena |
author_sort |
Delgado, Lídia |
title |
The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
title_short |
The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
title_full |
The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
title_fullStr |
The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
title_full_unstemmed |
The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography |
title_sort |
stacks: a new bacterial structure analyzed in the antarctic bacterium pseudomonas deceptionensis m1t by transmission electron microscopy and tomography |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
http://hdl.handle.net/2445/127822 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0073297 PLoS One, 2013, vol. 8, num. 9, p. e73297- Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1371/journal.pone.0073297 1932-6203 http://hdl.handle.net/2445/127822 628227 24039905 |
op_rights |
cc-by (c) Delgado, Lídia et al., 2013 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0073297 |
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PLoS ONE |
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8 |
container_issue |
9 |
container_start_page |
e73297 |
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