Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles....
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ftubarcepubl:oai:diposit.ub.edu:2445/104307 2024-02-11T09:55:48+01:00 Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. Pérez Cruz, Carla Delgado, Lídia López Iglesias, Carmen Mercadé Gil, M. Elena 2015-01-12 18 p. application/pdf http://hdl.handle.net/2445/104307 eng eng Public Library of Science (PLoS) Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0116896 PLoS One, 2015, vol. 10, num. 1, p. e0116896 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1371/journal.pone.0116896 1932-6203 http://hdl.handle.net/2445/104307 646213 25581302 cc-by (c) Pérez-Cruz, Carla et al., 2015 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Membranes cel·lulars Bacteris gramnegatius Bacteris patògens Cell membranes Gram-negative bacteria Pathogenic bacteria info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2015 ftubarcepubl https://doi.org/10.1371/journal.pone.0116896 2024-01-24T01:00:36Z Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles Article in Journal/Newspaper Antarc* Antarctic Dipòsit Digital de la Universitat de Barcelona Antarctic The Antarctic PLOS ONE 10 1 e0116896 |
institution |
Open Polar |
collection |
Dipòsit Digital de la Universitat de Barcelona |
op_collection_id |
ftubarcepubl |
language |
English |
topic |
Membranes cel·lulars Bacteris gramnegatius Bacteris patògens Cell membranes Gram-negative bacteria Pathogenic bacteria |
spellingShingle |
Membranes cel·lulars Bacteris gramnegatius Bacteris patògens Cell membranes Gram-negative bacteria Pathogenic bacteria Pérez Cruz, Carla Delgado, Lídia López Iglesias, Carmen Mercadé Gil, M. Elena Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
topic_facet |
Membranes cel·lulars Bacteris gramnegatius Bacteris patògens Cell membranes Gram-negative bacteria Pathogenic bacteria |
description |
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles |
format |
Article in Journal/Newspaper |
author |
Pérez Cruz, Carla Delgado, Lídia López Iglesias, Carmen Mercadé Gil, M. Elena |
author_facet |
Pérez Cruz, Carla Delgado, Lídia López Iglesias, Carmen Mercadé Gil, M. Elena |
author_sort |
Pérez Cruz, Carla |
title |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_short |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_full |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_fullStr |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_full_unstemmed |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_sort |
outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
http://hdl.handle.net/2445/104307 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0116896 PLoS One, 2015, vol. 10, num. 1, p. e0116896 Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) https://doi.org/10.1371/journal.pone.0116896 1932-6203 http://hdl.handle.net/2445/104307 646213 25581302 |
op_rights |
cc-by (c) Pérez-Cruz, Carla et al., 2015 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0116896 |
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PLOS ONE |
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10 |
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e0116896 |
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