Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas
Our society is increasingly concerned about the well-being of animals like dolphins, which can be affected by life under professional human care or by anthropogenic disturbances in the wild. To better understand and improve their welfare, scientists are exploring new, non-invasive methods to study t...
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ftuabarcelonapb:oai:ddd.uab.cat:293867 2024-09-15T17:59:04+00:00 Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas Agusti Pujol, Clara Manteca Vilanova, Xavier GarcÃa-Párraga, Daniel Talló Parra, Oriol 2024 application/pdf https://ddd.uab.cat/record/293867 eng eng Animals Vol. 14 (may 2024) https://ddd.uab.cat/record/293867 urn:10.3390/ani14091377 urn:oai:ddd.uab.cat:293867 urn:pmcid:PMC11083227 urn:pmc-uid:11083227 urn:pmid:38731382 urn:oai:pubmedcentral.nih.gov:11083227 urn:oai:egreta.uab.cat:publications/6b2be03d-a38a-467d-b51e-8bd06f467c30 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. https://creativecommons.org/licenses/by-nc-nd/4.0/ Cortisol Skin Stratum corneum Keratin Steroid hormones Cetacean Stress Conservation biology Article 2024 ftuabarcelonapb 2024-08-06T14:30:52Z Our society is increasingly concerned about the well-being of animals like dolphins, which can be affected by life under professional human care or by anthropogenic disturbances in the wild. To better understand and improve their welfare, scientists are exploring new, non-invasive methods to study the response to stressors in these animals. This research introduces an approach for measuring the stress response by analysing cortisol from epidermis samples, a method that does not require invasive procedures. We developed and tested a reliable technique to extract and measure cortisol levels from the epidermis of common bottlenose dolphins and belugas. Our findings show that this method works well and is accurate, even with very small epidermis samples. We also found that the amount of stress hormones can vary depending on the individual animal but not necessarily where the body sample was taken. This study is a step forward in non-invasively studying and enhancing the welfare of cetaceans, offering insights into their stress levels in a way that is safer and more comfortable for them, which could ultimately lead to better care and conservation practices for these species. Society is showing a growing concern about the welfare of cetaceans in captivity as well as cetaceans in the wild threatened by anthropogenic disturbances. The study of the physiological stress response is increasingly being used to address cetacean conservation and welfare issues. Within it, a newly described technique of extracting cortisol from epidermal desquamation may serve as a non-invasive, more integrated measure of a cetacean's stress response and welfare. However, confounding factors are common when measuring glucocorticoid hormones. In this study, we validated a steroid hormone extraction protocol and the use of a commercial enzyme immunoassay (EIA) test to measure cortisol concentrations in common bottlenose dolphin (Tursiops truncatus) and beluga (Delphinapterus leucas) epidermal samples. Moreover, we examined the effect of sample ... Article in Journal/Newspaper Beluga Beluga* Delphinapterus leucas Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
institution |
Open Polar |
collection |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Cortisol Skin Stratum corneum Keratin Steroid hormones Cetacean Stress Conservation biology |
spellingShingle |
Cortisol Skin Stratum corneum Keratin Steroid hormones Cetacean Stress Conservation biology Agusti Pujol, Clara Manteca Vilanova, Xavier GarcÃa-Párraga, Daniel Talló Parra, Oriol Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
topic_facet |
Cortisol Skin Stratum corneum Keratin Steroid hormones Cetacean Stress Conservation biology |
description |
Our society is increasingly concerned about the well-being of animals like dolphins, which can be affected by life under professional human care or by anthropogenic disturbances in the wild. To better understand and improve their welfare, scientists are exploring new, non-invasive methods to study the response to stressors in these animals. This research introduces an approach for measuring the stress response by analysing cortisol from epidermis samples, a method that does not require invasive procedures. We developed and tested a reliable technique to extract and measure cortisol levels from the epidermis of common bottlenose dolphins and belugas. Our findings show that this method works well and is accurate, even with very small epidermis samples. We also found that the amount of stress hormones can vary depending on the individual animal but not necessarily where the body sample was taken. This study is a step forward in non-invasively studying and enhancing the welfare of cetaceans, offering insights into their stress levels in a way that is safer and more comfortable for them, which could ultimately lead to better care and conservation practices for these species. Society is showing a growing concern about the welfare of cetaceans in captivity as well as cetaceans in the wild threatened by anthropogenic disturbances. The study of the physiological stress response is increasingly being used to address cetacean conservation and welfare issues. Within it, a newly described technique of extracting cortisol from epidermal desquamation may serve as a non-invasive, more integrated measure of a cetacean's stress response and welfare. However, confounding factors are common when measuring glucocorticoid hormones. In this study, we validated a steroid hormone extraction protocol and the use of a commercial enzyme immunoassay (EIA) test to measure cortisol concentrations in common bottlenose dolphin (Tursiops truncatus) and beluga (Delphinapterus leucas) epidermal samples. Moreover, we examined the effect of sample ... |
format |
Article in Journal/Newspaper |
author |
Agusti Pujol, Clara Manteca Vilanova, Xavier GarcÃa-Párraga, Daniel Talló Parra, Oriol |
author_facet |
Agusti Pujol, Clara Manteca Vilanova, Xavier GarcÃa-Párraga, Daniel Talló Parra, Oriol |
author_sort |
Agusti Pujol, Clara |
title |
Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
title_short |
Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
title_full |
Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
title_fullStr |
Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
title_full_unstemmed |
Validating a Non-Invasive Method for Assessing Cortisol Concentrations in Scraped Epidermal Skin from Common Bottlenose Dolphins and Belugas |
title_sort |
validating a non-invasive method for assessing cortisol concentrations in scraped epidermal skin from common bottlenose dolphins and belugas |
publishDate |
2024 |
url |
https://ddd.uab.cat/record/293867 |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_relation |
Animals Vol. 14 (may 2024) https://ddd.uab.cat/record/293867 urn:10.3390/ani14091377 urn:oai:ddd.uab.cat:293867 urn:pmcid:PMC11083227 urn:pmc-uid:11083227 urn:pmid:38731382 urn:oai:pubmedcentral.nih.gov:11083227 urn:oai:egreta.uab.cat:publications/6b2be03d-a38a-467d-b51e-8bd06f467c30 |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. https://creativecommons.org/licenses/by-nc-nd/4.0/ |
_version_ |
1810436001946927104 |