Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplem...
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ftuabarcelonapb:oai:ddd.uab.cat:260299 2023-05-15T13:58:11+02:00 Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos Ordóñez-León, Erika Alina Martínez-Rodero, Iris Garcia Martinez, Tania López Béjar, Manel Yeste Oliveras, Marc Mercadé, Elena Mogas Amorós, Teresa 2022 application/pdf https://ddd.uab.cat/record/260299 eng eng Ministerio de Ciencia e Innovación PID2020-116531RB-I00 Ministerio de Ciencia e Innovación BES-2017-081962 International journal of molecular sciences Vol. 23 Núm. 13 (2022), p. 7069 https://ddd.uab.cat/record/260299 urn:10.3390/ijms23137069 urn:oai:ddd.uab.cat:260299 urn:articleid:14220067v23n13p7069 urn:pmid:35806071 urn:pmc-uid:9266775 urn:pmcid:PMC9266775 urn:oai:pubmedcentral.nih.gov:9266775 urn:oai:egreta.uab.cat:publications/2cc2f00c-0bd6-45b8-98fe-47cb63aa55b2 urn:scopus_id:85132748245 urn:wos_id:000824297100001 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ CC-BY Cryopreservation Blastocyst Total cell number Inner cell mass TUNEL Embryo development Gene expression regulation Article 2022 ftuabarcelonapb 2023-02-06T21:26:11Z This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. Article in Journal/Newspaper Antarc* Antarctic Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB Antarctic |
institution |
Open Polar |
collection |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Cryopreservation Blastocyst Total cell number Inner cell mass TUNEL Embryo development Gene expression regulation |
spellingShingle |
Cryopreservation Blastocyst Total cell number Inner cell mass TUNEL Embryo development Gene expression regulation Ordóñez-León, Erika Alina Martínez-Rodero, Iris Garcia Martinez, Tania López Béjar, Manel Yeste Oliveras, Marc Mercadé, Elena Mogas Amorós, Teresa Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
topic_facet |
Cryopreservation Blastocyst Total cell number Inner cell mass TUNEL Embryo development Gene expression regulation |
description |
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. |
format |
Article in Journal/Newspaper |
author |
Ordóñez-León, Erika Alina Martínez-Rodero, Iris Garcia Martinez, Tania López Béjar, Manel Yeste Oliveras, Marc Mercadé, Elena Mogas Amorós, Teresa |
author_facet |
Ordóñez-León, Erika Alina Martínez-Rodero, Iris Garcia Martinez, Tania López Béjar, Manel Yeste Oliveras, Marc Mercadé, Elena Mogas Amorós, Teresa |
author_sort |
Ordóñez-León, Erika Alina |
title |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_short |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_full |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_fullStr |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_full_unstemmed |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_sort |
exopolysaccharide id1 improves post-warming outcomes after vitrification of in vitro-produced bovine embryos |
publishDate |
2022 |
url |
https://ddd.uab.cat/record/260299 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Ministerio de Ciencia e Innovación PID2020-116531RB-I00 Ministerio de Ciencia e Innovación BES-2017-081962 International journal of molecular sciences Vol. 23 Núm. 13 (2022), p. 7069 https://ddd.uab.cat/record/260299 urn:10.3390/ijms23137069 urn:oai:ddd.uab.cat:260299 urn:articleid:14220067v23n13p7069 urn:pmid:35806071 urn:pmc-uid:9266775 urn:pmcid:PMC9266775 urn:oai:pubmedcentral.nih.gov:9266775 urn:oai:egreta.uab.cat:publications/2cc2f00c-0bd6-45b8-98fe-47cb63aa55b2 urn:scopus_id:85132748245 urn:wos_id:000824297100001 |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
_version_ |
1766266283741937664 |