Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis
Piscirickettsia salmonis, an aggressive intracellular pathogen, is the etiological agent of salmonid rickettsial septicemia (SRS). This is a chronic multisystemic disease that generates high mortalities and large losses in Chilean salmon farming, threatening the sustainability of the salmon industry...
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ftuabarcelonapb:oai:ddd.uab.cat:253164 2024-09-15T17:55:58+00:00 Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis Pérez-Stuardo, Diego Espinoza, Allison Tapia, Sebastián Morales-Reyes, Jonathan Barrientos, Claudio Vallejos Vidal, Eva Carolina Sandino, Ana MarÃa Spencer, Eugenio Toro-Ascuy, Daniela Rivas-Pardo, J. Andrés Reyes-López, Felipe E. Reyes-Cerpa, Sebastián 2020 application/pdf https://ddd.uab.cat/record/253164 eng eng Frontiers in immunology Vol. 11 (November 2020), art. 544718 https://ddd.uab.cat/record/253164 urn:10.3389/fimmu.2020.544718 urn:oai:ddd.uab.cat:253164 urn:pmcid:PMC7688784 urn:pmc-uid:7688784 urn:pmid:33281810 urn:oai:pubmedcentral.nih.gov:7688784 urn:articleid:16643224v11p544718 urn:oai:egreta.uab.cat:publications/6a012cef-cad6-4c3b-93b3-e717504a0d4f open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ Macrophages Atlantic salmon IgM P. salmonis Lysosome activity Article 2020 ftuabarcelonapb 2024-08-06T14:30:50Z Piscirickettsia salmonis, an aggressive intracellular pathogen, is the etiological agent of salmonid rickettsial septicemia (SRS). This is a chronic multisystemic disease that generates high mortalities and large losses in Chilean salmon farming, threatening the sustainability of the salmon industry. Previous reports suggest that P. salmonis is able to survive and replicate in salmonid macrophages, inducing an anti-inflammatory environment and a limited lysosomal response that may be associated with host immune evasion mechanisms favoring bacterial survival. Current control and prophylaxis strategies against P. salmonis (based on the use of antibiotics and vaccines) have not had the expected success against infection. This makes it urgent to unravel the host-pathogen interaction to develop more effective therapeutic strategies. In this study, we evaluated the effect of treatment with IgM-beads on lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The impact of IgM-beads on cytotoxicity induced by P. salmonis in infected cells was evaluated by quantification of cell lysis through release of Lactate Dehydrogenase (LDH) activity. Bacterial load was determined by quantification of 16S rDNA copy number by qPCR, and counting of colony-forming units (CFU) present in the extracellular and intracellular environment. Our results suggest that stimulation with antibodies promotes lysosomal activity by lowering lysosomal pH and increasing the proteolytic activity within this organelle. Additionally, incubation with IgM-beads elicits a decrease in bacterial-induced cytotoxicity in infected Atlantic salmon macrophages and reduces the bacterial load. Overall, our results suggest that stimulation of cells infected by P. salmonis with IgM-beads reverses the modulation of the lysosomal activity induced by bacterial infection, promoting macrophage survival and bacterial elimination. This work represents ... Article in Journal/Newspaper Atlantic salmon Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
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Open Polar |
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Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Macrophages Atlantic salmon IgM P. salmonis Lysosome activity |
spellingShingle |
Macrophages Atlantic salmon IgM P. salmonis Lysosome activity Pérez-Stuardo, Diego Espinoza, Allison Tapia, Sebastián Morales-Reyes, Jonathan Barrientos, Claudio Vallejos Vidal, Eva Carolina Sandino, Ana MarÃa Spencer, Eugenio Toro-Ascuy, Daniela Rivas-Pardo, J. Andrés Reyes-López, Felipe E. Reyes-Cerpa, Sebastián Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
topic_facet |
Macrophages Atlantic salmon IgM P. salmonis Lysosome activity |
description |
Piscirickettsia salmonis, an aggressive intracellular pathogen, is the etiological agent of salmonid rickettsial septicemia (SRS). This is a chronic multisystemic disease that generates high mortalities and large losses in Chilean salmon farming, threatening the sustainability of the salmon industry. Previous reports suggest that P. salmonis is able to survive and replicate in salmonid macrophages, inducing an anti-inflammatory environment and a limited lysosomal response that may be associated with host immune evasion mechanisms favoring bacterial survival. Current control and prophylaxis strategies against P. salmonis (based on the use of antibiotics and vaccines) have not had the expected success against infection. This makes it urgent to unravel the host-pathogen interaction to develop more effective therapeutic strategies. In this study, we evaluated the effect of treatment with IgM-beads on lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The impact of IgM-beads on cytotoxicity induced by P. salmonis in infected cells was evaluated by quantification of cell lysis through release of Lactate Dehydrogenase (LDH) activity. Bacterial load was determined by quantification of 16S rDNA copy number by qPCR, and counting of colony-forming units (CFU) present in the extracellular and intracellular environment. Our results suggest that stimulation with antibodies promotes lysosomal activity by lowering lysosomal pH and increasing the proteolytic activity within this organelle. Additionally, incubation with IgM-beads elicits a decrease in bacterial-induced cytotoxicity in infected Atlantic salmon macrophages and reduces the bacterial load. Overall, our results suggest that stimulation of cells infected by P. salmonis with IgM-beads reverses the modulation of the lysosomal activity induced by bacterial infection, promoting macrophage survival and bacterial elimination. This work represents ... |
format |
Article in Journal/Newspaper |
author |
Pérez-Stuardo, Diego Espinoza, Allison Tapia, Sebastián Morales-Reyes, Jonathan Barrientos, Claudio Vallejos Vidal, Eva Carolina Sandino, Ana MarÃa Spencer, Eugenio Toro-Ascuy, Daniela Rivas-Pardo, J. Andrés Reyes-López, Felipe E. Reyes-Cerpa, Sebastián |
author_facet |
Pérez-Stuardo, Diego Espinoza, Allison Tapia, Sebastián Morales-Reyes, Jonathan Barrientos, Claudio Vallejos Vidal, Eva Carolina Sandino, Ana MarÃa Spencer, Eugenio Toro-Ascuy, Daniela Rivas-Pardo, J. Andrés Reyes-López, Felipe E. Reyes-Cerpa, Sebastián |
author_sort |
Pérez-Stuardo, Diego |
title |
Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
title_short |
Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
title_full |
Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
title_fullStr |
Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
title_full_unstemmed |
Non-Specific Antibodies Induce Lysosomal Activation in Atlantic Salmon Macrophages Infected by Piscirickettsia salmonis |
title_sort |
non-specific antibodies induce lysosomal activation in atlantic salmon macrophages infected by piscirickettsia salmonis |
publishDate |
2020 |
url |
https://ddd.uab.cat/record/253164 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Frontiers in immunology Vol. 11 (November 2020), art. 544718 https://ddd.uab.cat/record/253164 urn:10.3389/fimmu.2020.544718 urn:oai:ddd.uab.cat:253164 urn:pmcid:PMC7688784 urn:pmc-uid:7688784 urn:pmid:33281810 urn:oai:pubmedcentral.nih.gov:7688784 urn:articleid:16643224v11p544718 urn:oai:egreta.uab.cat:publications/6a012cef-cad6-4c3b-93b3-e717504a0d4f |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ |
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1810432180489289728 |