Diversity and distribution of marine heterotrophic bacteria from a large culture collection

• Main Authors: Sanz-Sáez, Isabel, Salazar, Guillem, Sánchez, Pablo, Lara, Elena, Royo-Llonch, Marta, Sà, Elisabet L., Lucena, Teresa, Pujalte, María J., Vaqué, Dolors, Duarte, Carlos M., Gasol, Josep M., Pedrós-Alió, Carlos, Sánchez Martínez, M. Olga, Acinas, Silvia G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2020
• Subjects: Bacterial isolates; Deep ocean; Photic ocean; Diversity; Arctic; Indian; Pacific; South Atlantic Ocean
• Online Access: https://ddd.uab.cat/record/238787

Altres ajuts: grant BIOSENSOMICS "Convocatoria 2015 de ayudas Fundación BBVA a investigadores y creadores culturales" Altres ajuts: Analyses were also possible thank to the King Abdullah University of Science and Technology (KAUST) through baseline funding to C.M.D. and a subaward agreement OSR-2014-CC-1973-02 between KAUST and Universitat Autònoma de Barcelona (UAB) Background: Isolation of marine microorganisms is fundamental to gather information about their physiology, ecology and genomic content. To date, most of the bacterial isolation efforts have focused on the photic ocean leaving the deep ocean less explored. We have created a marine culture collection of heterotrophic bacteria (MARINHET) using a standard marine medium comprising a total of 1561 bacterial strains, and covering a variety of oceanographic regions from different seasons and years, from 2009 to 2015. Specifically, our marine collection contains isolates from both photic (817) and aphotic layers (744), including the mesopelagic (362) and the bathypelagic (382), from the North Western Mediterranean Sea, the North and South Atlantic Ocean, the Indian, the Pacific, and the Arctic Oceans. We described the taxonomy, the phylogenetic diversity and the biogeography of a fraction of the marine culturable microorganisms to enhance our knowledge about which heterotrophic marine isolates are recurrently retrieved across oceans and along different depths. Results: The partial sequencing of the 16S rRNA gene of all isolates revealed that they mainly affiliate with the classes Alphaproteobacteria (35.9%), Gammaproteobacteria (38.6%), and phylum Bacteroidetes (16.5%). In addition, Alteromonas and Erythrobacter genera were found the most common heterotrophic bacteria in the ocean growing in solid agar medium. When comparing all photic, mesopelagic, and bathypelagic isolates sequences retrieved from different stations, 37% of them were 100% identical. This percentage increased up to 59% when mesopelagic and bathypelagic strains were grouped as the ...