An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus
Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen- associated molecular...
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ftuabarcelonapb:oai:ddd.uab.cat:225169 2023-07-02T03:32:10+02:00 An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus Callol, Agnes Reyes-López, Felipe E. Roig, Francisco J. Goetz, Giles Goetz, Frederick W. Amaro, Carmen Mackenzie, Simon 2015 application/pdf https://ddd.uab.cat/record/225169 eng eng Ministerio de Ciencia e Innovación AGL2011-29639 Ministerio de Ciencia e Innovación CSD2009-00006 Ministerio de Ciencia e Innovación CSD2007-00002 Ministerio de Ciencia e Innovación BES-2009-024776 PloS one Vol. 10, issue 7 (2015), art. e0133328 https://ddd.uab.cat/record/225169 urn:10.1371/journal.pone.0133328 urn:oai:ddd.uab.cat:225169 urn:pmid:26207370 urn:scopus_id:84941712144 urn:articleid:19326203v10n7e0133328 urn:oai:egreta.uab.cat:publications/64f4d683-6aca-484a-809d-0f51cf4dae62 urn:pmc-uid:4514713 urn:pmcid:PMC4514713 urn:oai:pubmedcentral.nih.gov:4514713 open access Aquest document està subjecte a una llicència d'ús Creative Commons en que s'entrega l'obra al domini públic. Podeu copiar, modificar, distribuir l'obra i fer-ne comunicació pública, fins i tot amb finalitat comercial, sense demanar cap mena de permís. https://creativecommons.org/publicdomain/zero/1.0/ Animals Eels Fish diseases Host-pathogen interactions Signal transduction Transcriptome Vibrio infections Vibrio vulnificus Article 2015 ftuabarcelonapb 2023-06-13T23:51:59Z Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen- associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome.We obtained more than 2x10 6 reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA1 3 ) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA1 3 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immuneresponse is rtxA1 3 -dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue. Article in Journal/Newspaper European eel Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB The Portal ENVELOPE(159.167,159.167,-78.100,-78.100) PLOS ONE 10 7 e0133328 |
institution |
Open Polar |
collection |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Animals Eels Fish diseases Host-pathogen interactions Signal transduction Transcriptome Vibrio infections Vibrio vulnificus |
spellingShingle |
Animals Eels Fish diseases Host-pathogen interactions Signal transduction Transcriptome Vibrio infections Vibrio vulnificus Callol, Agnes Reyes-López, Felipe E. Roig, Francisco J. Goetz, Giles Goetz, Frederick W. Amaro, Carmen Mackenzie, Simon An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
topic_facet |
Animals Eels Fish diseases Host-pathogen interactions Signal transduction Transcriptome Vibrio infections Vibrio vulnificus |
description |
Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen- associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome.We obtained more than 2x10 6 reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA1 3 ) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA1 3 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immuneresponse is rtxA1 3 -dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue. |
format |
Article in Journal/Newspaper |
author |
Callol, Agnes Reyes-López, Felipe E. Roig, Francisco J. Goetz, Giles Goetz, Frederick W. Amaro, Carmen Mackenzie, Simon |
author_facet |
Callol, Agnes Reyes-López, Felipe E. Roig, Francisco J. Goetz, Giles Goetz, Frederick W. Amaro, Carmen Mackenzie, Simon |
author_sort |
Callol, Agnes |
title |
An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
title_short |
An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
title_full |
An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
title_fullStr |
An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
title_full_unstemmed |
An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus |
title_sort |
enriched european eel transcriptome sheds light upon host-pathogen interactions with vibrio vulnificus |
publishDate |
2015 |
url |
https://ddd.uab.cat/record/225169 |
long_lat |
ENVELOPE(159.167,159.167,-78.100,-78.100) |
geographic |
The Portal |
geographic_facet |
The Portal |
genre |
European eel |
genre_facet |
European eel |
op_relation |
Ministerio de Ciencia e Innovación AGL2011-29639 Ministerio de Ciencia e Innovación CSD2009-00006 Ministerio de Ciencia e Innovación CSD2007-00002 Ministerio de Ciencia e Innovación BES-2009-024776 PloS one Vol. 10, issue 7 (2015), art. e0133328 https://ddd.uab.cat/record/225169 urn:10.1371/journal.pone.0133328 urn:oai:ddd.uab.cat:225169 urn:pmid:26207370 urn:scopus_id:84941712144 urn:articleid:19326203v10n7e0133328 urn:oai:egreta.uab.cat:publications/64f4d683-6aca-484a-809d-0f51cf4dae62 urn:pmc-uid:4514713 urn:pmcid:PMC4514713 urn:oai:pubmedcentral.nih.gov:4514713 |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons en que s'entrega l'obra al domini públic. Podeu copiar, modificar, distribuir l'obra i fer-ne comunicació pública, fins i tot amb finalitat comercial, sense demanar cap mena de permís. https://creativecommons.org/publicdomain/zero/1.0/ |
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PLOS ONE |
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10 |
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7 |
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