Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus)
DNA vaccines encoding the viral G glycoprotein show the most successful protection capability against fish rhabdoviruses. Nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. With the aim of shedding light on the protection conferred by the DNA vaccin...
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ftuabarcelonapb:oai:ddd.uab.cat:225159 2023-07-02T03:33:40+02:00 Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) Pereiro, Patricia Dios, Sonia Boltana, Sebastian Coll, Julio Estepa, Amparo Mackenzie, Simon Novoa, Beatriz Figueras, Antonio 2014 application/pdf https://ddd.uab.cat/record/225159 eng eng Ministerio de Ciencia e Innovación AGL2011-28921-C03 Ministerio de Ciencia e Innovación CSD2007-00002 Ministerio de Educación y Ciencia AP2010-2408 PloS one Vol. 9 issue 8 (2014), art. e104509 https://ddd.uab.cat/record/225159 urn:10.1371/journal.pone.0104509 urn:oai:ddd.uab.cat:225159 urn:pmid:25098168 urn:scopus_id:84905493180 urn:articleid:19326203v9n8e104509 urn:oai:egreta.uab.cat:publications/cd90a3cd-1278-4e50-aee3-f5a2a7e643a4 urn:pmc-uid:4123995 urn:pmcid:PMC4123995 urn:oai:pubmedcentral.nih.gov:4123995 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ Animals Fish diseases Flatfishes Novirhabdovirus Rhabdoviridae infections Transcriptome Vaccines DNA Viral vaccines Article 2014 ftuabarcelonapb 2023-06-13T23:51:59Z DNA vaccines encoding the viral G glycoprotein show the most successful protection capability against fish rhabdoviruses. Nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. With the aim of shedding light on the protection conferred by the DNA vaccines based in the G glycoprotein of viral haemorrhagic septicaemia virus (VHSV) in turbot (Scophthalmus maximus) we have used a specific microarray highly enriched in antiviral sequences to carry out the transcriptomic study associated to VHSV DNA vaccination/infection. The differential gene expression pattern in response to empty plasmid (pMCV1.4) and DNA vaccine (pMCV1.4-G) intramuscular administration with regard to non-stimulated turbot was analyzed in head kidney at 8, 24 and 72 hours post-vaccination. Moreover, the effect of VHSV infection one month after immunization was also analyzed in vaccinated and non-vaccinated fish at the same time points. Genes implicated in the Toll-like receptor signalling pathway, IFN inducible/regulatory proteins, numerous sequences implicated in apoptosis and cytotoxic pathways, MHC class I antigens, as well as complement and coagulation cascades among others were analyzed in the different experimental groups. Fish receiving the pMCV1.4-G vaccine showed transcriptomic patterns very different to the ones observed in pMCV1.4-injected turbot after 72 h. On the other hand, VHSV challenge in vaccinated and non-vaccinated turbot induced a highly different response at the transcriptome level, indicating a very relevant role of the acquired immunity in vaccinated fish able to alter the typical innate immune response profile observed in non-vaccinated individuals. This exhaustive transcriptome study will serve as a complete overview for a better understanding of the crosstalk between the innate and adaptive immune response in fish after viral infection/vaccination. Moreover, it provides interesting clues about molecules with a potential use as vaccine adjuvants, antiviral treatments or ... Article in Journal/Newspaper Scophthalmus maximus Turbot Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB PLoS ONE 9 8 e104509 |
institution |
Open Polar |
collection |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Animals Fish diseases Flatfishes Novirhabdovirus Rhabdoviridae infections Transcriptome Vaccines DNA Viral vaccines |
spellingShingle |
Animals Fish diseases Flatfishes Novirhabdovirus Rhabdoviridae infections Transcriptome Vaccines DNA Viral vaccines Pereiro, Patricia Dios, Sonia Boltana, Sebastian Coll, Julio Estepa, Amparo Mackenzie, Simon Novoa, Beatriz Figueras, Antonio Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
topic_facet |
Animals Fish diseases Flatfishes Novirhabdovirus Rhabdoviridae infections Transcriptome Vaccines DNA Viral vaccines |
description |
DNA vaccines encoding the viral G glycoprotein show the most successful protection capability against fish rhabdoviruses. Nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. With the aim of shedding light on the protection conferred by the DNA vaccines based in the G glycoprotein of viral haemorrhagic septicaemia virus (VHSV) in turbot (Scophthalmus maximus) we have used a specific microarray highly enriched in antiviral sequences to carry out the transcriptomic study associated to VHSV DNA vaccination/infection. The differential gene expression pattern in response to empty plasmid (pMCV1.4) and DNA vaccine (pMCV1.4-G) intramuscular administration with regard to non-stimulated turbot was analyzed in head kidney at 8, 24 and 72 hours post-vaccination. Moreover, the effect of VHSV infection one month after immunization was also analyzed in vaccinated and non-vaccinated fish at the same time points. Genes implicated in the Toll-like receptor signalling pathway, IFN inducible/regulatory proteins, numerous sequences implicated in apoptosis and cytotoxic pathways, MHC class I antigens, as well as complement and coagulation cascades among others were analyzed in the different experimental groups. Fish receiving the pMCV1.4-G vaccine showed transcriptomic patterns very different to the ones observed in pMCV1.4-injected turbot after 72 h. On the other hand, VHSV challenge in vaccinated and non-vaccinated turbot induced a highly different response at the transcriptome level, indicating a very relevant role of the acquired immunity in vaccinated fish able to alter the typical innate immune response profile observed in non-vaccinated individuals. This exhaustive transcriptome study will serve as a complete overview for a better understanding of the crosstalk between the innate and adaptive immune response in fish after viral infection/vaccination. Moreover, it provides interesting clues about molecules with a potential use as vaccine adjuvants, antiviral treatments or ... |
format |
Article in Journal/Newspaper |
author |
Pereiro, Patricia Dios, Sonia Boltana, Sebastian Coll, Julio Estepa, Amparo Mackenzie, Simon Novoa, Beatriz Figueras, Antonio |
author_facet |
Pereiro, Patricia Dios, Sonia Boltana, Sebastian Coll, Julio Estepa, Amparo Mackenzie, Simon Novoa, Beatriz Figueras, Antonio |
author_sort |
Pereiro, Patricia |
title |
Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
title_short |
Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
title_full |
Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
title_fullStr |
Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
title_full_unstemmed |
Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus) |
title_sort |
transcriptome profiles associated to vhsv infection or dna vaccination in turbot (scophthalmus maximus) |
publishDate |
2014 |
url |
https://ddd.uab.cat/record/225159 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Ministerio de Ciencia e Innovación AGL2011-28921-C03 Ministerio de Ciencia e Innovación CSD2007-00002 Ministerio de Educación y Ciencia AP2010-2408 PloS one Vol. 9 issue 8 (2014), art. e104509 https://ddd.uab.cat/record/225159 urn:10.1371/journal.pone.0104509 urn:oai:ddd.uab.cat:225159 urn:pmid:25098168 urn:scopus_id:84905493180 urn:articleid:19326203v9n8e104509 urn:oai:egreta.uab.cat:publications/cd90a3cd-1278-4e50-aee3-f5a2a7e643a4 urn:pmc-uid:4123995 urn:pmcid:PMC4123995 urn:oai:pubmedcentral.nih.gov:4123995 |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ |
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