Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis
Altres ajuts: FONDECYT/11150807, CORFO 13CTI-21527, 101205 Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://ddd.uab.cat/record/224166 |
id |
ftuabarcelonapb:oai:ddd.uab.cat:224166 |
---|---|
record_format |
openpolar |
spelling |
ftuabarcelonapb:oai:ddd.uab.cat:224166 2024-09-15T17:55:45+00:00 Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis Pérez-Stuardo, Diego Morales-Reyes, Jonathan Tapia, Sebastián Ahumada, Diego E. Espinoza, Allison Soto-Herrera, Valentina Brianson, Bernardo Ibaceta, Valentina Sandino, Ana MarÃa Spencer, Eugenio Vallejos Vidal, Eva Carolina Reyes-López, Felipe E. Valdés, Jorge Reyes-Cerpa, Sebastián 2019 application/pdf https://ddd.uab.cat/record/224166 eng eng Frontiers in immunology Vol. 10 (March 2019), art. 434 https://ddd.uab.cat/record/224166 urn:10.3389/fimmu.2019.00434 urn:oai:ddd.uab.cat:224166 urn:scopus_id:85064230830 urn:articleid:16643224v10p434 urn:oai:egreta.uab.cat:publications/a498cf5e-fda1-43e2-aa50-ceb01b0d585f urn:pmid:30941123 urn:pmc-uid:6433878 urn:pmcid:PMC6433878 urn:oai:pubmedcentral.nih.gov:6433878 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ Atlantic salmon (Salmo salar) Piscirickettsiosis Immune evasion mechanisms Macrophages Lysosome Proteolytic activity Article 2019 ftuabarcelonapb 2024-08-06T14:30:49Z Altres ajuts: FONDECYT/11150807, CORFO 13CTI-21527, 101205 Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P. salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P. salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P. salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P. salmonis can survive ≥120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P. salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P. salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P. salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P. salmonis that have not been previously reported. Article in Journal/Newspaper Atlantic salmon Salmo salar Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB Frontiers in Immunology 10 |
institution |
Open Polar |
collection |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Atlantic salmon (Salmo salar) Piscirickettsiosis Immune evasion mechanisms Macrophages Lysosome Proteolytic activity |
spellingShingle |
Atlantic salmon (Salmo salar) Piscirickettsiosis Immune evasion mechanisms Macrophages Lysosome Proteolytic activity Pérez-Stuardo, Diego Morales-Reyes, Jonathan Tapia, Sebastián Ahumada, Diego E. Espinoza, Allison Soto-Herrera, Valentina Brianson, Bernardo Ibaceta, Valentina Sandino, Ana MarÃa Spencer, Eugenio Vallejos Vidal, Eva Carolina Reyes-López, Felipe E. Valdés, Jorge Reyes-Cerpa, Sebastián Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
topic_facet |
Atlantic salmon (Salmo salar) Piscirickettsiosis Immune evasion mechanisms Macrophages Lysosome Proteolytic activity |
description |
Altres ajuts: FONDECYT/11150807, CORFO 13CTI-21527, 101205 Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P. salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P. salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P. salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P. salmonis can survive ≥120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P. salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P. salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P. salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P. salmonis that have not been previously reported. |
format |
Article in Journal/Newspaper |
author |
Pérez-Stuardo, Diego Morales-Reyes, Jonathan Tapia, Sebastián Ahumada, Diego E. Espinoza, Allison Soto-Herrera, Valentina Brianson, Bernardo Ibaceta, Valentina Sandino, Ana MarÃa Spencer, Eugenio Vallejos Vidal, Eva Carolina Reyes-López, Felipe E. Valdés, Jorge Reyes-Cerpa, Sebastián |
author_facet |
Pérez-Stuardo, Diego Morales-Reyes, Jonathan Tapia, Sebastián Ahumada, Diego E. Espinoza, Allison Soto-Herrera, Valentina Brianson, Bernardo Ibaceta, Valentina Sandino, Ana MarÃa Spencer, Eugenio Vallejos Vidal, Eva Carolina Reyes-López, Felipe E. Valdés, Jorge Reyes-Cerpa, Sebastián |
author_sort |
Pérez-Stuardo, Diego |
title |
Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
title_short |
Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
title_full |
Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
title_fullStr |
Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
title_full_unstemmed |
Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis |
title_sort |
non-lysosomal activation in macrophages of atlantic salmon (salmo salar) after infection with piscirickettsia salmonis |
publishDate |
2019 |
url |
https://ddd.uab.cat/record/224166 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Frontiers in immunology Vol. 10 (March 2019), art. 434 https://ddd.uab.cat/record/224166 urn:10.3389/fimmu.2019.00434 urn:oai:ddd.uab.cat:224166 urn:scopus_id:85064230830 urn:articleid:16643224v10p434 urn:oai:egreta.uab.cat:publications/a498cf5e-fda1-43e2-aa50-ceb01b0d585f urn:pmid:30941123 urn:pmc-uid:6433878 urn:pmcid:PMC6433878 urn:oai:pubmedcentral.nih.gov:6433878 |
op_rights |
open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ |
container_title |
Frontiers in Immunology |
container_volume |
10 |
_version_ |
1810431988760313856 |