Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A
This work was supported by ERANET-IB08-007 project from the European Union and its linked national project EUI2008- 03610 to AV. We also appreciate the support from EME2007-08 to NFM from Universitat Autonoma de Barcelona, from Antartide 2010 to MLT and EP, from MIUR Azioni Integrate Italia-Spagna 2...
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ftuabarcelonapb:oai:ddd.uab.cat:132802 2023-05-15T14:17:02+02:00 Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A Unzueta Elorza, Ugutz Vázquez Lima, Felícitas Accardi, Giulia Mendoza, Rosa Toledo-Rubio, Verónica Giuliani, Maria Sannino, Filomena Parrilli, Ermenegilda Abasolo, Ibane Schwartz, Simo Villaverde Corrales, Antonio Corchero Nieto, José Luis Ferrer-Miralles, Neus Tutino, Maria L. 2015 application/pdf https://ddd.uab.cat/record/132802 eng eng Ministerio de Economía y Competitividad IT2009-0021 Ministerio de Economía y Competitividad EUI2008-03610 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-108 Applied microbiology and biotechnology Vol 99, Issue 14 (July 2015), p. 5863-5874 https://ddd.uab.cat/record/132802 urn:10.1007/s00253-014-6328-9 urn:oai:ddd.uab.cat:132802 urn:recercauab:ARE-78977 urn:articleid:01757598v99n14p5863 urn:scopus_id:84932198319 urn:wos_id:000356806300010 urn:oai:egreta.uab.cat:publications/245fa017-2d4b-4b73-92b5-0090c42d6b52 open access Tots els drets reservats. https://rightsstatements.org/vocab/InC/1.0/ Recombinant protein Expression systems Escherichia coli Pseudoalteromonas haloplanktis TAC125 Human alpha-galactosidase A Fabry's disease Article 2015 ftuabarcelonapb 2023-02-06T21:03:29Z This work was supported by ERANET-IB08-007 project from the European Union and its linked national project EUI2008- 03610 to AV. We also appreciate the support from EME2007-08 to NFM from Universitat Autonoma de Barcelona, from Antartide 2010 to MLT and EP, from MIUR Azioni Integrate Italia-Spagna 2010 Prot. IT10LECLM9 to MLT, from MINECO (IT2009-0021) to AV and LT, from AGAUR (2009SGR-108) to AV. AV is also supported by The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN, Spain), an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. PS has received predoctoral fellowship from ISCIII, and AV has been distinguished with an ICREA ACADEMIA award (Catalonia, Spain). Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the available recombinant expression systems, Escherichia coli facilitates gene expression by its relative simplicity, inexpensive and fast cultivation, well-known genetics and the large number of tools available for its biotechnological application. However, recombinant expression in E. coli is not always a straightforward procedure and major obstacles are encountered when producing many eukaryotic proteins and especially membrane proteins, linked to missing posttranslational modifications, proteolysis and aggregation. In this context, many conventional and unconventional eukaryotic hosts are under exploration and development, but in some cases linked to complex culture media or processes. In this context, alternative bacterial systems able to overcome some of the limitations posed by E. coli keeping the simplicity of prokaryotic manipulation are currently emerging as convenient hosts for protein production. We have comparatively produced a "difficult-to-express" human protein, the lysosomal ... Article in Journal/Newspaper Antartide Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB Applied Microbiology and Biotechnology 99 14 5863 5874 |
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Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
op_collection_id |
ftuabarcelonapb |
language |
English |
topic |
Recombinant protein Expression systems Escherichia coli Pseudoalteromonas haloplanktis TAC125 Human alpha-galactosidase A Fabry's disease |
spellingShingle |
Recombinant protein Expression systems Escherichia coli Pseudoalteromonas haloplanktis TAC125 Human alpha-galactosidase A Fabry's disease Unzueta Elorza, Ugutz Vázquez Lima, Felícitas Accardi, Giulia Mendoza, Rosa Toledo-Rubio, Verónica Giuliani, Maria Sannino, Filomena Parrilli, Ermenegilda Abasolo, Ibane Schwartz, Simo Villaverde Corrales, Antonio Corchero Nieto, José Luis Ferrer-Miralles, Neus Tutino, Maria L. Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
topic_facet |
Recombinant protein Expression systems Escherichia coli Pseudoalteromonas haloplanktis TAC125 Human alpha-galactosidase A Fabry's disease |
description |
This work was supported by ERANET-IB08-007 project from the European Union and its linked national project EUI2008- 03610 to AV. We also appreciate the support from EME2007-08 to NFM from Universitat Autonoma de Barcelona, from Antartide 2010 to MLT and EP, from MIUR Azioni Integrate Italia-Spagna 2010 Prot. IT10LECLM9 to MLT, from MINECO (IT2009-0021) to AV and LT, from AGAUR (2009SGR-108) to AV. AV is also supported by The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN, Spain), an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. PS has received predoctoral fellowship from ISCIII, and AV has been distinguished with an ICREA ACADEMIA award (Catalonia, Spain). Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the available recombinant expression systems, Escherichia coli facilitates gene expression by its relative simplicity, inexpensive and fast cultivation, well-known genetics and the large number of tools available for its biotechnological application. However, recombinant expression in E. coli is not always a straightforward procedure and major obstacles are encountered when producing many eukaryotic proteins and especially membrane proteins, linked to missing posttranslational modifications, proteolysis and aggregation. In this context, many conventional and unconventional eukaryotic hosts are under exploration and development, but in some cases linked to complex culture media or processes. In this context, alternative bacterial systems able to overcome some of the limitations posed by E. coli keeping the simplicity of prokaryotic manipulation are currently emerging as convenient hosts for protein production. We have comparatively produced a "difficult-to-express" human protein, the lysosomal ... |
format |
Article in Journal/Newspaper |
author |
Unzueta Elorza, Ugutz Vázquez Lima, Felícitas Accardi, Giulia Mendoza, Rosa Toledo-Rubio, Verónica Giuliani, Maria Sannino, Filomena Parrilli, Ermenegilda Abasolo, Ibane Schwartz, Simo Villaverde Corrales, Antonio Corchero Nieto, José Luis Ferrer-Miralles, Neus Tutino, Maria L. |
author_facet |
Unzueta Elorza, Ugutz Vázquez Lima, Felícitas Accardi, Giulia Mendoza, Rosa Toledo-Rubio, Verónica Giuliani, Maria Sannino, Filomena Parrilli, Ermenegilda Abasolo, Ibane Schwartz, Simo Villaverde Corrales, Antonio Corchero Nieto, José Luis Ferrer-Miralles, Neus Tutino, Maria L. |
author_sort |
Unzueta Elorza, Ugutz |
title |
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
title_short |
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
title_full |
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
title_fullStr |
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
title_full_unstemmed |
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A |
title_sort |
strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase a |
publishDate |
2015 |
url |
https://ddd.uab.cat/record/132802 |
genre |
Antartide |
genre_facet |
Antartide |
op_relation |
Ministerio de Economía y Competitividad IT2009-0021 Ministerio de Economía y Competitividad EUI2008-03610 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-108 Applied microbiology and biotechnology Vol 99, Issue 14 (July 2015), p. 5863-5874 https://ddd.uab.cat/record/132802 urn:10.1007/s00253-014-6328-9 urn:oai:ddd.uab.cat:132802 urn:recercauab:ARE-78977 urn:articleid:01757598v99n14p5863 urn:scopus_id:84932198319 urn:wos_id:000356806300010 urn:oai:egreta.uab.cat:publications/245fa017-2d4b-4b73-92b5-0090c42d6b52 |
op_rights |
open access Tots els drets reservats. https://rightsstatements.org/vocab/InC/1.0/ |
container_title |
Applied Microbiology and Biotechnology |
container_volume |
99 |
container_issue |
14 |
container_start_page |
5863 |
op_container_end_page |
5874 |
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1766288987697184768 |