From low-throughput SSR genotyping to high-throughput SNP analyses of natural populations: validation of their application focused on non-invasive samples

Efficient genetics tools are needed to provide information on the genetic constitution of endangered species. DNA quantification as a means of quality assessment of non-invasive genetic samples and a novel approach of SNP detection in non-model species called Random Amplicon Sequencing (RAMseq) were...

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Bibliographic Details
Main Author: Bayerl, Helmut
Other Authors: Kühn, Ralph (Prof. Dr.), Gula, Roman (Prof. Dr.), Geist, Jürgen (Prof. Dr.)
Format: Doctoral or Postdoctoral Thesis
Language:English
Published: Technical University of Munich 2019
Subjects:
microsatellite
SSR
single nucleotide polymorphism
SNP
conservation genetics
non-invasive genetic sampling
Eurasian otter
Lutra lutra
RAMseq
Mikrosatellit
Punktmutation
Naturschutzgenetik
non-invasive genetische Probenahme
Eurasischer Fischotter
ddc:570
Biowissenschaften
Biologie
Fischotter
Online Access:https://mediatum.ub.tum.de/doc/1497679/document.pdf
https://mediatum.ub.tum.de/node?id=1497679
https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20190926-1497679-1-7
Description
Summary:Efficient genetics tools are needed to provide information on the genetic constitution of endangered species. DNA quantification as a means of quality assessment of non-invasive genetic samples and a novel approach of SNP detection in non-model species called Random Amplicon Sequencing (RAMseq) were developed. These were validated during a non-invasive genetic census of the Eurasian otter ( Lutra lutra ) and performance of developed SNP markers in population genetic analyses was compared to that of established microsatellites. Effektive genetische Methoden werden benötigt, um Informationen über die genetische Konstitution bedrohter Arten zu erhalten. Die DNA-Quantifizierung zur Qualitätsbestimmung non-invasiver Proben und eine neue Methode zur Detektion von SNP Markern in nicht-Modelltierarten, Random Amplicon Sequencing (RAMseq), wurden entwickelt. Diese wurden während eines non-invasiven genetischen Zensus des Eurasischen Fischotters ( Lutra lutra ) validiert und die Effizienz der entwickelten SNP Marker mit der von etablierten Mikrosatelliten verglichen.

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