Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance
The use of immobilized enzymes as renewable biocatalysts is gaining attention in the scientific community. The immobilization of enzymes is necessary to avoid a complex separation and recovery of the enzymes and to also apply enzymes in an Enzymatic Reactive Distillation (ERD). The ERD helps to furt...
Published in: | Chemical Engineering and Processing: Process Intensification |
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Language: | English |
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Online Access: | http://hdl.handle.net/11420/8219 |
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fttuhamburg:oai:tore.tuhh.de:11420/8219 2023-08-20T04:02:30+02:00 Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance Wierschem, Matthias Walz, Olga Mitsos, Alexander Termuehlen, Maren Specht, Anna Lena Kissing, Katrin Skiborowski, Mirko 2017-01 http://hdl.handle.net/11420/8219 en eng Chemical engineering and processing 0255-2701 Chemical Engineering and Processing: Process Intensification (111): 25-34 (2017-01) http://hdl.handle.net/11420/8219 2-s2.0-84995767719 Biocatalysis Enzymatic reactive distillation Kinetic modeling Process intensification Sonochemistry Journal Article Other 2017 fttuhamburg 2023-07-28T09:22:44Z The use of immobilized enzymes as renewable biocatalysts is gaining attention in the scientific community. The immobilization of enzymes is necessary to avoid a complex separation and recovery of the enzymes and to also apply enzymes in an Enzymatic Reactive Distillation (ERD). The ERD helps to further overcome the equilibrium limitations of transesterification reactions by means of in-situ product separation. Potential mass transfer limitations caused by the immobilization can be overcome by ultrasound (US) application to form the US-assisted ERD (US-ERD). In order to evaluate the application of ERD and US-ERD this article presents the experimental investigation of the transesterification of ethyl butyrate catalyzed by the enzyme Candida antarctica lipase B for two different types of immobilizate (coated packing and enzyme beads) with and without US-assistance. Low frequency US tends to destroy the immobilizates whereas high frequency US can increase the rate of an enzymatic reaction by 50% for the coated packings. Enzyme beads do not show diffusion limitation whereas mass transfer limitations for the coated packing can be reduced by US application. A kinetic model is identified for each of the immobilizates with and without US application and the parameters of the models accurately fit to experimental data. Article in Journal/Newspaper Antarc* Antarctica TUHH Open Research (TORE - Technische Universität Hamburg) Chemical Engineering and Processing: Process Intensification 111 25 34 |
institution |
Open Polar |
collection |
TUHH Open Research (TORE - Technische Universität Hamburg) |
op_collection_id |
fttuhamburg |
language |
English |
topic |
Biocatalysis Enzymatic reactive distillation Kinetic modeling Process intensification Sonochemistry |
spellingShingle |
Biocatalysis Enzymatic reactive distillation Kinetic modeling Process intensification Sonochemistry Wierschem, Matthias Walz, Olga Mitsos, Alexander Termuehlen, Maren Specht, Anna Lena Kissing, Katrin Skiborowski, Mirko Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
topic_facet |
Biocatalysis Enzymatic reactive distillation Kinetic modeling Process intensification Sonochemistry |
description |
The use of immobilized enzymes as renewable biocatalysts is gaining attention in the scientific community. The immobilization of enzymes is necessary to avoid a complex separation and recovery of the enzymes and to also apply enzymes in an Enzymatic Reactive Distillation (ERD). The ERD helps to further overcome the equilibrium limitations of transesterification reactions by means of in-situ product separation. Potential mass transfer limitations caused by the immobilization can be overcome by ultrasound (US) application to form the US-assisted ERD (US-ERD). In order to evaluate the application of ERD and US-ERD this article presents the experimental investigation of the transesterification of ethyl butyrate catalyzed by the enzyme Candida antarctica lipase B for two different types of immobilizate (coated packing and enzyme beads) with and without US-assistance. Low frequency US tends to destroy the immobilizates whereas high frequency US can increase the rate of an enzymatic reaction by 50% for the coated packings. Enzyme beads do not show diffusion limitation whereas mass transfer limitations for the coated packing can be reduced by US application. A kinetic model is identified for each of the immobilizates with and without US application and the parameters of the models accurately fit to experimental data. |
format |
Article in Journal/Newspaper |
author |
Wierschem, Matthias Walz, Olga Mitsos, Alexander Termuehlen, Maren Specht, Anna Lena Kissing, Katrin Skiborowski, Mirko |
author_facet |
Wierschem, Matthias Walz, Olga Mitsos, Alexander Termuehlen, Maren Specht, Anna Lena Kissing, Katrin Skiborowski, Mirko |
author_sort |
Wierschem, Matthias |
title |
Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
title_short |
Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
title_full |
Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
title_fullStr |
Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
title_full_unstemmed |
Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
title_sort |
enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance |
publishDate |
2017 |
url |
http://hdl.handle.net/11420/8219 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Chemical engineering and processing 0255-2701 Chemical Engineering and Processing: Process Intensification (111): 25-34 (2017-01) http://hdl.handle.net/11420/8219 2-s2.0-84995767719 |
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Chemical Engineering and Processing: Process Intensification |
container_volume |
111 |
container_start_page |
25 |
op_container_end_page |
34 |
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1774712975708389376 |