Pseudoalteromonas arctica sp. nov., an aerobic, psychrotolerant, marine bacterium isolated from Spitzbergen

• Published in: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
• Main Authors: Khudary, Rami al, Stößer, Nele Isabelle, Ooura, Farah, Antranikian, Garabed
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2008
• Subjects: 570: Biowissenschaften; Biologie; Arctic; Spitzbergen
• Online Access: http://hdl.handle.net/11420/4421

A novel aerobic, psychrotolerant marine bacterium was isolated at 4 °C from seawater samples collected from Spitzbergen in the Arctic. The strain was a polar-flagellated, Gram-negative bacterium that grew optimally at 10-15 °C and pH 7-8 in media containing 2-3% NaCl (w/v), using various carbohydrates and organic acids as substrates. The main fatty acid components included 16 : 0 (12.7% of total fatty acids), straight-chain saturated fatty acid methyl ester (FAME) and 16 : 1ω7c (40.2 %) monounsaturated FAME. Phylogenetic analysis revealed a close relationship (99% 16S rRNA gene sequence similarity) between the novel isolate and Pseudoalteromonas elyakovii KMM 162T and some other species of the genus Pseudoalteromonas. The DNA G+C content of the novel strain was 39 mol%. DNA-DNA hybridization showed only 47.6% DNA-DNA relatedness with P. elyakovii KMM 162T, 44.2% with Pseudoalteromonas distincta KMM 638T and 22.6% with Pseudoalteromonas nigrifaciens NCIMB 8614T. Based on phylogenetic and phenotypic characteristics, this isolate represents a novel species of the genus Pseudoalteromonas for which the name Pseudoalteromonas arctica is proposed; the type strain is A 37-1-2T (=LMG 23753T=DSM 18437T). © 2008 IUMS.