Bacillus subtilis lipase a—lipase or esterase?
The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a...
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Online Access: | http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 https://doi.org/10.3390/catal10030308 |
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fttudelft:oai:tudelft.nl:uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 2024-04-28T08:02:57+00:00 Bacillus subtilis lipase a—lipase or esterase? Bracco Garcia, M.P. (author) van Midden, Nelleke (author) Arango, Epifanía (author) Torrelo, Guzman (author) Ferrario, Valerio (author) Gardossi, Lucia (author) Hanefeld, U. (author) 2020 http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 https://doi.org/10.3390/catal10030308 en eng http://www.scopus.com/inward/record.url?scp=85081209222&partnerID=8YFLogxK Catalysts--2073-4344--06aac578-d8aa-4449-9a10-70f5ab4660cd http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 https://doi.org/10.3390/catal10030308 © 2020 M.P. Bracco Garcia, Nelleke van Midden, Epifanía Arango, Guzman Torrelo, Valerio Ferrario, Lucia Gardossi, U. Hanefeld Bacillus subtilis lipase A Esterase Hydrolase Lipase Organic solvent Transesterification Water activity journal article 2020 fttudelft https://doi.org/10.3390/catal10030308 2024-04-09T23:59:36Z The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to distinguish between lipases and esterases. Lipases tend to be active in dry organic solvents, while esterases are not active under these circumstances. BT/Biocatalysis Article in Journal/Newspaper Antarc* Antarctica Delft University of Technology: Institutional Repository Catalysts 10 3 308 |
institution |
Open Polar |
collection |
Delft University of Technology: Institutional Repository |
op_collection_id |
fttudelft |
language |
English |
topic |
Bacillus subtilis lipase A Esterase Hydrolase Lipase Organic solvent Transesterification Water activity |
spellingShingle |
Bacillus subtilis lipase A Esterase Hydrolase Lipase Organic solvent Transesterification Water activity Bracco Garcia, M.P. (author) van Midden, Nelleke (author) Arango, Epifanía (author) Torrelo, Guzman (author) Ferrario, Valerio (author) Gardossi, Lucia (author) Hanefeld, U. (author) Bacillus subtilis lipase a—lipase or esterase? |
topic_facet |
Bacillus subtilis lipase A Esterase Hydrolase Lipase Organic solvent Transesterification Water activity |
description |
The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to distinguish between lipases and esterases. Lipases tend to be active in dry organic solvents, while esterases are not active under these circumstances. BT/Biocatalysis |
format |
Article in Journal/Newspaper |
author |
Bracco Garcia, M.P. (author) van Midden, Nelleke (author) Arango, Epifanía (author) Torrelo, Guzman (author) Ferrario, Valerio (author) Gardossi, Lucia (author) Hanefeld, U. (author) |
author_facet |
Bracco Garcia, M.P. (author) van Midden, Nelleke (author) Arango, Epifanía (author) Torrelo, Guzman (author) Ferrario, Valerio (author) Gardossi, Lucia (author) Hanefeld, U. (author) |
author_sort |
Bracco Garcia, M.P. (author) |
title |
Bacillus subtilis lipase a—lipase or esterase? |
title_short |
Bacillus subtilis lipase a—lipase or esterase? |
title_full |
Bacillus subtilis lipase a—lipase or esterase? |
title_fullStr |
Bacillus subtilis lipase a—lipase or esterase? |
title_full_unstemmed |
Bacillus subtilis lipase a—lipase or esterase? |
title_sort |
bacillus subtilis lipase a—lipase or esterase? |
publishDate |
2020 |
url |
http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 https://doi.org/10.3390/catal10030308 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.scopus.com/inward/record.url?scp=85081209222&partnerID=8YFLogxK Catalysts--2073-4344--06aac578-d8aa-4449-9a10-70f5ab4660cd http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 https://doi.org/10.3390/catal10030308 |
op_rights |
© 2020 M.P. Bracco Garcia, Nelleke van Midden, Epifanía Arango, Guzman Torrelo, Valerio Ferrario, Lucia Gardossi, U. Hanefeld |
op_doi |
https://doi.org/10.3390/catal10030308 |
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Catalysts |
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10 |
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3 |
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308 |
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1797574192587079680 |