Bacillus subtilis lipase a—lipase or esterase?

The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a...

Full description

Bibliographic Details
Published in:Catalysts
Main Authors: Bracco Garcia, M.P. (author), van Midden, Nelleke (author), Arango, Epifanía (author), Torrelo, Guzman (author), Ferrario, Valerio (author), Gardossi, Lucia (author), Hanefeld, U. (author)
Format: Article in Journal/Newspaper
Language:English
Published: 2020
Subjects:
Bacillus subtilis lipase A
Esterase
Hydrolase
Lipase
Organic solvent
Transesterification
Water activity
Antarc*
Antarctica
Online Access:http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2
https://doi.org/10.3390/catal10030308
Description
Summary:The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to distinguish between lipases and esterases. Lipases tend to be active in dry organic solvents, while esterases are not active under these circumstances. BT/Biocatalysis

Similar Items