Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet
Abstract Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conse...
Published in: | Environmental DNA |
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Wiley
2021
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Online Access: | https://doi.org/10.1002/edn3.237 https://doaj.org/article/f202579e1acd4d7ea5ec47ba2ce1dc37 |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:f202579e1acd4d7ea5ec47ba2ce1dc37 2023-05-15T16:13:46+02:00 Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet Physilia Ying Shi Chua Youri Lammers Emmanuel Menoni Torbjørn Ekrem Kristine Bohmann Sanne Boessenkool Inger Greve Alsos 2021-11-01 https://doi.org/10.1002/edn3.237 https://doaj.org/article/f202579e1acd4d7ea5ec47ba2ce1dc37 en eng Wiley 2637-4943 doi:10.1002/edn3.237 https://doaj.org/article/f202579e1acd4d7ea5ec47ba2ce1dc37 undefined Environmental DNA, Vol 3, Iss 6, Pp 1156-1171 (2021) ecology environmental DNA grouse herbivory high‐throughput sequencing envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2021 fttriple https://doi.org/10.1002/edn3.237 2023-01-22T19:22:55Z Abstract Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non‐molecular means used to study their diet are time‐consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non‐invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large‐scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non‐molecular means. The non‐invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. Article in Journal/Newspaper Finnmark Finnmark Troms Unknown Norway Jura ENVELOPE(13.501,13.501,68.062,68.062) Environmental DNA 3 6 1156 1171 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ecology environmental DNA grouse herbivory high‐throughput sequencing envir geo |
spellingShingle |
ecology environmental DNA grouse herbivory high‐throughput sequencing envir geo Physilia Ying Shi Chua Youri Lammers Emmanuel Menoni Torbjørn Ekrem Kristine Bohmann Sanne Boessenkool Inger Greve Alsos Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
topic_facet |
ecology environmental DNA grouse herbivory high‐throughput sequencing envir geo |
description |
Abstract Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non‐molecular means used to study their diet are time‐consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non‐invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large‐scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non‐molecular means. The non‐invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. |
format |
Article in Journal/Newspaper |
author |
Physilia Ying Shi Chua Youri Lammers Emmanuel Menoni Torbjørn Ekrem Kristine Bohmann Sanne Boessenkool Inger Greve Alsos |
author_facet |
Physilia Ying Shi Chua Youri Lammers Emmanuel Menoni Torbjørn Ekrem Kristine Bohmann Sanne Boessenkool Inger Greve Alsos |
author_sort |
Physilia Ying Shi Chua |
title |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_short |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_fullStr |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full_unstemmed |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_sort |
molecular dietary analyses of western capercaillies (tetrao urogallus) reveal a diverse diet |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doi.org/10.1002/edn3.237 https://doaj.org/article/f202579e1acd4d7ea5ec47ba2ce1dc37 |
long_lat |
ENVELOPE(13.501,13.501,68.062,68.062) |
geographic |
Norway Jura |
geographic_facet |
Norway Jura |
genre |
Finnmark Finnmark Troms |
genre_facet |
Finnmark Finnmark Troms |
op_source |
Environmental DNA, Vol 3, Iss 6, Pp 1156-1171 (2021) |
op_relation |
2637-4943 doi:10.1002/edn3.237 https://doaj.org/article/f202579e1acd4d7ea5ec47ba2ce1dc37 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1002/edn3.237 |
container_title |
Environmental DNA |
container_volume |
3 |
container_issue |
6 |
container_start_page |
1156 |
op_container_end_page |
1171 |
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1765999604060389376 |