Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production
Affiliations of the dominant culturable bacteria isolated from Potter Cove, South Shetland Islands, Antarctica, were investigated together with their production of cold-active hydrolytic enzymes. A total of 189 aerobic heterotrophic bacterial isolates were obtained at 4°C and sorted into 63 phylotyp...
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Norwegian Polar Institute
2012
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Online Access: | https://doi.org/10.3402/polar.v31i0.18507 https://doaj.org/article/f1f3a91be7df40048855db1143dcaaab |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:f1f3a91be7df40048855db1143dcaaab 2023-05-15T14:04:02+02:00 Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production Mauro Tropeano Silvia Coria Adrián Turjanski Daniel Cicero Andrés Bercovich Walter Mac Cormack Susana Vázquez 2012-12-01 https://doi.org/10.3402/polar.v31i0.18507 https://doaj.org/article/f1f3a91be7df40048855db1143dcaaab en eng Norwegian Polar Institute doi:10.3402/polar.v31i0.18507 0800-0395 1751-8369 https://doaj.org/article/f1f3a91be7df40048855db1143dcaaab undefined Polar Research, Vol 31, Iss 0, Pp 1-11 (2012) Microbial enzymes Antarctic bacteria marine bacteria cold enzymes psychrophiles geo envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2012 fttriple https://doi.org/10.3402/polar.v31i0.18507 2023-01-22T19:08:29Z Affiliations of the dominant culturable bacteria isolated from Potter Cove, South Shetland Islands, Antarctica, were investigated together with their production of cold-active hydrolytic enzymes. A total of 189 aerobic heterotrophic bacterial isolates were obtained at 4°C and sorted into 63 phylotypes based on their amplified ribosomal DNA restriction analysis profiles. The sequencing of the 16S rRNA genes of representatives from each phylotype showed that the isolates belong to the phyla Proteobacteria (classes Alpha- and Gamma-proteobacteria), Bacteroidetes (class Flavobacteria), Actinobacteria (class Actinobacteria) and Firmicutes (class Bacilli). The predominant culturable group in the site studied belongs to the class Gammaproteobacteria, with 65 isolates affiliated to the genus Pseudoalteromonas and 58 to Psychrobacter. Among the 189 isolates screened, producers of amylases (9.5%), pectinases (22.8%), cellulases (14.8%), CM-cellulases (25.4%), xylanases (20.1%) and proteases (44.4%) were detected. More than 25% of the isolates produced at least one extracellular enzyme, with some of them producing up to six of the tested extracellular enzymatic activities. These results suggest that a high culturable bacterial diversity is present in Potter Cove and that this place represents a promising source of biomolecules. Article in Journal/Newspaper Antarc* Antarctic Antarctica Polar Research South Shetland Islands Unknown Antarctic Potter Cove South Shetland Islands Polar Research 31 1 18507 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Microbial enzymes Antarctic bacteria marine bacteria cold enzymes psychrophiles geo envir |
spellingShingle |
Microbial enzymes Antarctic bacteria marine bacteria cold enzymes psychrophiles geo envir Mauro Tropeano Silvia Coria Adrián Turjanski Daniel Cicero Andrés Bercovich Walter Mac Cormack Susana Vázquez Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
topic_facet |
Microbial enzymes Antarctic bacteria marine bacteria cold enzymes psychrophiles geo envir |
description |
Affiliations of the dominant culturable bacteria isolated from Potter Cove, South Shetland Islands, Antarctica, were investigated together with their production of cold-active hydrolytic enzymes. A total of 189 aerobic heterotrophic bacterial isolates were obtained at 4°C and sorted into 63 phylotypes based on their amplified ribosomal DNA restriction analysis profiles. The sequencing of the 16S rRNA genes of representatives from each phylotype showed that the isolates belong to the phyla Proteobacteria (classes Alpha- and Gamma-proteobacteria), Bacteroidetes (class Flavobacteria), Actinobacteria (class Actinobacteria) and Firmicutes (class Bacilli). The predominant culturable group in the site studied belongs to the class Gammaproteobacteria, with 65 isolates affiliated to the genus Pseudoalteromonas and 58 to Psychrobacter. Among the 189 isolates screened, producers of amylases (9.5%), pectinases (22.8%), cellulases (14.8%), CM-cellulases (25.4%), xylanases (20.1%) and proteases (44.4%) were detected. More than 25% of the isolates produced at least one extracellular enzyme, with some of them producing up to six of the tested extracellular enzymatic activities. These results suggest that a high culturable bacterial diversity is present in Potter Cove and that this place represents a promising source of biomolecules. |
format |
Article in Journal/Newspaper |
author |
Mauro Tropeano Silvia Coria Adrián Turjanski Daniel Cicero Andrés Bercovich Walter Mac Cormack Susana Vázquez |
author_facet |
Mauro Tropeano Silvia Coria Adrián Turjanski Daniel Cicero Andrés Bercovich Walter Mac Cormack Susana Vázquez |
author_sort |
Mauro Tropeano |
title |
Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
title_short |
Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
title_full |
Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
title_fullStr |
Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
title_full_unstemmed |
Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production |
title_sort |
culturable heterotrophic bacteria from potter cove, antarctica, and their hydrolytic enzymes production |
publisher |
Norwegian Polar Institute |
publishDate |
2012 |
url |
https://doi.org/10.3402/polar.v31i0.18507 https://doaj.org/article/f1f3a91be7df40048855db1143dcaaab |
geographic |
Antarctic Potter Cove South Shetland Islands |
geographic_facet |
Antarctic Potter Cove South Shetland Islands |
genre |
Antarc* Antarctic Antarctica Polar Research South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica Polar Research South Shetland Islands |
op_source |
Polar Research, Vol 31, Iss 0, Pp 1-11 (2012) |
op_relation |
doi:10.3402/polar.v31i0.18507 0800-0395 1751-8369 https://doaj.org/article/f1f3a91be7df40048855db1143dcaaab |
op_rights |
undefined |
op_doi |
https://doi.org/10.3402/polar.v31i0.18507 |
container_title |
Polar Research |
container_volume |
31 |
container_issue |
1 |
container_start_page |
18507 |
_version_ |
1766274977552662528 |