A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chroma...
Published in: | Ocean Science |
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Copernicus Publications
2020
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Online Access: | https://doi.org/10.5194/os-16-817-2020 https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.pdf https://doaj.org/article/ec77485215034c0b92941ee3e5db9879 |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:ec77485215034c0b92941ee3e5db9879 2023-05-15T15:06:13+02:00 A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD S. Zeppenfeld M. van Pinxteren A. Engel H. Herrmann 2020-07-01 https://doi.org/10.5194/os-16-817-2020 https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.pdf https://doaj.org/article/ec77485215034c0b92941ee3e5db9879 en eng Copernicus Publications doi:10.5194/os-16-817-2020 1812-0784 1812-0792 https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.pdf https://doaj.org/article/ec77485215034c0b92941ee3e5db9879 undefined Ocean Science, Vol 16, Pp 817-830 (2020) envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.5194/os-16-817-2020 2023-01-22T19:25:15Z An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. Article in Journal/Newspaper Arctic ice core Unknown Arctic Ocean Science 16 4 817 830 |
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English |
topic |
envir geo |
spellingShingle |
envir geo S. Zeppenfeld M. van Pinxteren A. Engel H. Herrmann A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
topic_facet |
envir geo |
description |
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. |
format |
Article in Journal/Newspaper |
author |
S. Zeppenfeld M. van Pinxteren A. Engel H. Herrmann |
author_facet |
S. Zeppenfeld M. van Pinxteren A. Engel H. Herrmann |
author_sort |
S. Zeppenfeld |
title |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_short |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_fullStr |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full_unstemmed |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_sort |
protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ed) – combined with hpaec-pad |
publisher |
Copernicus Publications |
publishDate |
2020 |
url |
https://doi.org/10.5194/os-16-817-2020 https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.pdf https://doaj.org/article/ec77485215034c0b92941ee3e5db9879 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic ice core |
genre_facet |
Arctic ice core |
op_source |
Ocean Science, Vol 16, Pp 817-830 (2020) |
op_relation |
doi:10.5194/os-16-817-2020 1812-0784 1812-0792 https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.pdf https://doaj.org/article/ec77485215034c0b92941ee3e5db9879 |
op_rights |
undefined |
op_doi |
https://doi.org/10.5194/os-16-817-2020 |
container_title |
Ocean Science |
container_volume |
16 |
container_issue |
4 |
container_start_page |
817 |
op_container_end_page |
830 |
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1766337866792697856 |