Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.)
Abstract Molecular techniques offer sensitive, specific, noninvasive monitoring of target species from a variety of environmental samples. We recently developed a CRISPR‐Cas‐based eDNA assay for rapid single‐species detection as a route to a simple, cost‐effective biosensor device. CRISPR‐Cas‐based...
Published in: | Environmental DNA |
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Online Access: | https://doi.org/10.1002/edn3.174 https://doaj.org/article/e4e87ffdd3424e0888e29f94802e91da |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:e4e87ffdd3424e0888e29f94802e91da 2023-05-15T15:32:15+02:00 Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) Molly‐Ann Williams Cecilia Hernandez Antóin M. O'Sullivan Julien April Fiona Regan Louis Bernatchez Anne Parle‐McDermott 2021-01-01 https://doi.org/10.1002/edn3.174 https://doaj.org/article/e4e87ffdd3424e0888e29f94802e91da en eng Wiley 2637-4943 doi:10.1002/edn3.174 https://doaj.org/article/e4e87ffdd3424e0888e29f94802e91da undefined Environmental DNA, Vol 3, Iss 1, Pp 297-304 (2021) CRISPR‐Cas eDNA fresh water qPCR Salmo salar envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2021 fttriple https://doi.org/10.1002/edn3.174 2023-01-22T19:28:08Z Abstract Molecular techniques offer sensitive, specific, noninvasive monitoring of target species from a variety of environmental samples. We recently developed a CRISPR‐Cas‐based eDNA assay for rapid single‐species detection as a route to a simple, cost‐effective biosensor device. CRISPR‐Cas‐based diagnostic assays use isothermal conditions in combination with a highly specific sequence recognition system. This CRISPR‐Cas assay was designed to target Salmo salar, and we previously demonstrated its utility in eDNA samples from sites in Ireland. The aim of this study was to validate our assay in two larger sample sets from Canada (n = 16/n = 63) in comparison with an independent S. salar qPCR assay. We demonstrate that overall, the CRISPR‐Cas assay performs similarly to qPCR for assessing the presence or absence of S. salar from eDNA and provides a viable alternative approach where qPCR assay design and application have proven to be challenging. Article in Journal/Newspaper Atlantic salmon Salmo salar Unknown Canada Environmental DNA 3 1 297 304 |
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Open Polar |
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language |
English |
topic |
CRISPR‐Cas eDNA fresh water qPCR Salmo salar envir geo |
spellingShingle |
CRISPR‐Cas eDNA fresh water qPCR Salmo salar envir geo Molly‐Ann Williams Cecilia Hernandez Antóin M. O'Sullivan Julien April Fiona Regan Louis Bernatchez Anne Parle‐McDermott Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
topic_facet |
CRISPR‐Cas eDNA fresh water qPCR Salmo salar envir geo |
description |
Abstract Molecular techniques offer sensitive, specific, noninvasive monitoring of target species from a variety of environmental samples. We recently developed a CRISPR‐Cas‐based eDNA assay for rapid single‐species detection as a route to a simple, cost‐effective biosensor device. CRISPR‐Cas‐based diagnostic assays use isothermal conditions in combination with a highly specific sequence recognition system. This CRISPR‐Cas assay was designed to target Salmo salar, and we previously demonstrated its utility in eDNA samples from sites in Ireland. The aim of this study was to validate our assay in two larger sample sets from Canada (n = 16/n = 63) in comparison with an independent S. salar qPCR assay. We demonstrate that overall, the CRISPR‐Cas assay performs similarly to qPCR for assessing the presence or absence of S. salar from eDNA and provides a viable alternative approach where qPCR assay design and application have proven to be challenging. |
format |
Article in Journal/Newspaper |
author |
Molly‐Ann Williams Cecilia Hernandez Antóin M. O'Sullivan Julien April Fiona Regan Louis Bernatchez Anne Parle‐McDermott |
author_facet |
Molly‐Ann Williams Cecilia Hernandez Antóin M. O'Sullivan Julien April Fiona Regan Louis Bernatchez Anne Parle‐McDermott |
author_sort |
Molly‐Ann Williams |
title |
Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
title_short |
Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
title_full |
Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
title_fullStr |
Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) |
title_sort |
comparing crispr‐cas and qpcr edna assays for the detection of atlantic salmon (salmo salar l.) |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doi.org/10.1002/edn3.174 https://doaj.org/article/e4e87ffdd3424e0888e29f94802e91da |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Environmental DNA, Vol 3, Iss 1, Pp 297-304 (2021) |
op_relation |
2637-4943 doi:10.1002/edn3.174 https://doaj.org/article/e4e87ffdd3424e0888e29f94802e91da |
op_rights |
undefined |
op_doi |
https://doi.org/10.1002/edn3.174 |
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Environmental DNA |
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3 |
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1 |
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297 |
op_container_end_page |
304 |
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