An alternative method for correcting fluorescence quenching
Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Souther...
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2015
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fttriple:oai:gotriple.eu:oai:doaj.org/article:de73be1d08094651abc76b4573a52dfc 2023-05-15T16:05:43+02:00 An alternative method for correcting fluorescence quenching L. Biermann C. Guinet M. Bester A. Brierley L. Boehme 2015-01-01 https://doi.org/10.5194/os-11-83-2015 http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/article/de73be1d08094651abc76b4573a52dfc en eng Copernicus Publications 1812-0784 1812-0792 doi:10.5194/os-11-83-2015 http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/article/de73be1d08094651abc76b4573a52dfc undefined Ocean Science, Vol 11, Iss 1, Pp 83-91 (2015) envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.5194/os-11-83-2015 2023-01-22T19:34:33Z Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. Article in Journal/Newspaper Elephant Seals Southern Elephant Seals Southern Ocean Unknown Austral Southern Ocean Ocean Science 11 1 83 91 |
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English |
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envir geo |
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envir geo L. Biermann C. Guinet M. Bester A. Brierley L. Boehme An alternative method for correcting fluorescence quenching |
topic_facet |
envir geo |
description |
Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. |
format |
Article in Journal/Newspaper |
author |
L. Biermann C. Guinet M. Bester A. Brierley L. Boehme |
author_facet |
L. Biermann C. Guinet M. Bester A. Brierley L. Boehme |
author_sort |
L. Biermann |
title |
An alternative method for correcting fluorescence quenching |
title_short |
An alternative method for correcting fluorescence quenching |
title_full |
An alternative method for correcting fluorescence quenching |
title_fullStr |
An alternative method for correcting fluorescence quenching |
title_full_unstemmed |
An alternative method for correcting fluorescence quenching |
title_sort |
alternative method for correcting fluorescence quenching |
publisher |
Copernicus Publications |
publishDate |
2015 |
url |
https://doi.org/10.5194/os-11-83-2015 http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/article/de73be1d08094651abc76b4573a52dfc |
geographic |
Austral Southern Ocean |
geographic_facet |
Austral Southern Ocean |
genre |
Elephant Seals Southern Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Elephant Seals Southern Ocean |
op_source |
Ocean Science, Vol 11, Iss 1, Pp 83-91 (2015) |
op_relation |
1812-0784 1812-0792 doi:10.5194/os-11-83-2015 http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/article/de73be1d08094651abc76b4573a52dfc |
op_rights |
undefined |
op_doi |
https://doi.org/10.5194/os-11-83-2015 |
container_title |
Ocean Science |
container_volume |
11 |
container_issue |
1 |
container_start_page |
83 |
op_container_end_page |
91 |
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1766401615053455360 |