Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice
Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures...
Published in: | Elementa: Science of the Anthropocene |
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2020
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Online Access: | https://doi.org/10.1525/elementa.421 https://doaj.org/article/da7fb732b9844c30b63141e97ce7c66f |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:da7fb732b9844c30b63141e97ce7c66f 2023-05-15T13:58:27+02:00 Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice Hannah M. Dawson Katherine R. Heal Angela K. Boysen Laura T. Carlson Anitra E. Ingalls Jodi N. Young 2020-06-01 https://doi.org/10.1525/elementa.421 https://doaj.org/article/da7fb732b9844c30b63141e97ce7c66f en eng BioOne 2325-1026 doi:10.1525/elementa.421 https://doaj.org/article/da7fb732b9844c30b63141e97ce7c66f undefined Elementa: Science of the Anthropocene, Vol 8, Iss 1 (2020) sea ice metabolomics diatom osmoprotection cryoprotection metabolism envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.1525/elementa.421 2023-01-22T18:10:38Z Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, 'Nitzschia lecointei', displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2, 3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4-fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, had the highest intracellular concentrations among all compatible solutes of ~85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. Article in Journal/Newspaper Antarc* Antarctic Arctic ice algae Sea ice Unknown Antarctic Arctic The Antarctic Elementa: Science of the Anthropocene 8 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
sea ice metabolomics diatom osmoprotection cryoprotection metabolism envir geo |
spellingShingle |
sea ice metabolomics diatom osmoprotection cryoprotection metabolism envir geo Hannah M. Dawson Katherine R. Heal Angela K. Boysen Laura T. Carlson Anitra E. Ingalls Jodi N. Young Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
topic_facet |
sea ice metabolomics diatom osmoprotection cryoprotection metabolism envir geo |
description |
Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, 'Nitzschia lecointei', displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2, 3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4-fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, had the highest intracellular concentrations among all compatible solutes of ~85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
format |
Article in Journal/Newspaper |
author |
Hannah M. Dawson Katherine R. Heal Angela K. Boysen Laura T. Carlson Anitra E. Ingalls Jodi N. Young |
author_facet |
Hannah M. Dawson Katherine R. Heal Angela K. Boysen Laura T. Carlson Anitra E. Ingalls Jodi N. Young |
author_sort |
Hannah M. Dawson |
title |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_short |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_full |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_fullStr |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_full_unstemmed |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_sort |
potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
publisher |
BioOne |
publishDate |
2020 |
url |
https://doi.org/10.1525/elementa.421 https://doaj.org/article/da7fb732b9844c30b63141e97ce7c66f |
geographic |
Antarctic Arctic The Antarctic |
geographic_facet |
Antarctic Arctic The Antarctic |
genre |
Antarc* Antarctic Arctic ice algae Sea ice |
genre_facet |
Antarc* Antarctic Arctic ice algae Sea ice |
op_source |
Elementa: Science of the Anthropocene, Vol 8, Iss 1 (2020) |
op_relation |
2325-1026 doi:10.1525/elementa.421 https://doaj.org/article/da7fb732b9844c30b63141e97ce7c66f |
op_rights |
undefined |
op_doi |
https://doi.org/10.1525/elementa.421 |
container_title |
Elementa: Science of the Anthropocene |
container_volume |
8 |
_version_ |
1766266758670319616 |