Culturable diversity of Arctic phytoplankton during pack ice melting
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometr...
Published in: | Elementa: Science of the Anthropocene |
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Online Access: | https://doi.org/10.1525/elementa.401 https://doaj.org/article/d97e427755e24d1cac91e66c3d9e1d4c |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:d97e427755e24d1cac91e66c3d9e1d4c 2023-05-15T14:45:37+02:00 Culturable diversity of Arctic phytoplankton during pack ice melting Catherine Gérikas Ribeiro Adriana Lopes dos Santos Priscillia Gourvil Florence Le Gall Dominique Marie Margot Tragin Ian Probert Daniel Vaulot 2020-02-01 https://doi.org/10.1525/elementa.401 https://doaj.org/article/d97e427755e24d1cac91e66c3d9e1d4c en eng BioOne 2325-1026 doi:10.1525/elementa.401 https://doaj.org/article/d97e427755e24d1cac91e66c3d9e1d4c undefined Elementa: Science of the Anthropocene, Vol 8, Iss 1 (2020) arctic diatoms arctic flagellates isolation techniques 18s rrna roscoff culture collection sympagic phytoplankton communities envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.1525/elementa.401 2023-01-22T19:28:10Z Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera 'Chaetoceros and Attheya'. The genera 'Baffinella and Pyramimonas' were the most abundant non-diatom nanoplankton strains, while 'Micromonas polaris' dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera 'Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis'. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. Article in Journal/Newspaper Arctic Baffin Bay Baffin Bay Baffin Phytoplankton Unknown Arctic Baffin Bay Elementa: Science of the Anthropocene 8 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
arctic diatoms arctic flagellates isolation techniques 18s rrna roscoff culture collection sympagic phytoplankton communities envir geo |
spellingShingle |
arctic diatoms arctic flagellates isolation techniques 18s rrna roscoff culture collection sympagic phytoplankton communities envir geo Catherine Gérikas Ribeiro Adriana Lopes dos Santos Priscillia Gourvil Florence Le Gall Dominique Marie Margot Tragin Ian Probert Daniel Vaulot Culturable diversity of Arctic phytoplankton during pack ice melting |
topic_facet |
arctic diatoms arctic flagellates isolation techniques 18s rrna roscoff culture collection sympagic phytoplankton communities envir geo |
description |
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera 'Chaetoceros and Attheya'. The genera 'Baffinella and Pyramimonas' were the most abundant non-diatom nanoplankton strains, while 'Micromonas polaris' dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera 'Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis'. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. |
format |
Article in Journal/Newspaper |
author |
Catherine Gérikas Ribeiro Adriana Lopes dos Santos Priscillia Gourvil Florence Le Gall Dominique Marie Margot Tragin Ian Probert Daniel Vaulot |
author_facet |
Catherine Gérikas Ribeiro Adriana Lopes dos Santos Priscillia Gourvil Florence Le Gall Dominique Marie Margot Tragin Ian Probert Daniel Vaulot |
author_sort |
Catherine Gérikas Ribeiro |
title |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_short |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_fullStr |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full_unstemmed |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_sort |
culturable diversity of arctic phytoplankton during pack ice melting |
publisher |
BioOne |
publishDate |
2020 |
url |
https://doi.org/10.1525/elementa.401 https://doaj.org/article/d97e427755e24d1cac91e66c3d9e1d4c |
geographic |
Arctic Baffin Bay |
geographic_facet |
Arctic Baffin Bay |
genre |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
genre_facet |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
op_source |
Elementa: Science of the Anthropocene, Vol 8, Iss 1 (2020) |
op_relation |
2325-1026 doi:10.1525/elementa.401 https://doaj.org/article/d97e427755e24d1cac91e66c3d9e1d4c |
op_rights |
undefined |
op_doi |
https://doi.org/10.1525/elementa.401 |
container_title |
Elementa: Science of the Anthropocene |
container_volume |
8 |
_version_ |
1766317009135468544 |