Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean
Algal chlorophyllous pigments were extracted with two different solvents, N, N-dimethylformamide (DMF) and 90% acetone, using natural phytoplankton communities in the Southern Ocean. Concentrations of chlorophyll α and phaeopigments were determined fluorometrically. The former solvent was employed d...
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National Institute of Polar Research
1993
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fttriple:oai:gotriple.eu:oai:doaj.org/article:cecaf63607e34d8996b69ac31477507d 2023-05-15T14:03:52+02:00 Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean Tsuneo Odate Naomi Harada Mitsuo Fukuchi 1993-11-01 https://doi.org/10.15094/00008821 https://doaj.org/article/cecaf63607e34d8996b69ac31477507d en other eng National Institute of Polar Research doi:10.15094/00008821 0085-7289 2432-079X https://doaj.org/article/cecaf63607e34d8996b69ac31477507d undefined Antarctic Record, Vol 37, Iss 3, Pp 260-264 (1993) geo envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 1993 fttriple https://doi.org/10.15094/00008821 2023-01-22T17:53:03Z Algal chlorophyllous pigments were extracted with two different solvents, N, N-dimethylformamide (DMF) and 90% acetone, using natural phytoplankton communities in the Southern Ocean. Concentrations of chlorophyll α and phaeopigments were determined fluorometrically. The former solvent was employed during the 33rd Japanese Antarctic Research Expedition (JARE-33) while the latter has been used during previous JAREs. Statistical analyses showed that chlorophyll α concentrations were equivalent although the solvents were different. No significant difference was observed between concentrations of phaeopigments extracted with the different solvents either. The correlation coefficient obtained between phaeopigments concentrations was significantly lower than that between chlorophyll α concentrations. Phaeopigment concentrations extracted with DMF tended to be higher than those with 90% acetone. Total chlorophyllous pigments (chlorophyll α plus phaeopigments) were about 10% higher in the former solvent than in the latter, considering the slope of the regression line (=1.088). Article in Journal/Newspaper Antarc* Antarctic Southern Ocean Unknown Antarctic Southern Ocean |
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language |
English |
topic |
geo envir |
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geo envir Tsuneo Odate Naomi Harada Mitsuo Fukuchi Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
topic_facet |
geo envir |
description |
Algal chlorophyllous pigments were extracted with two different solvents, N, N-dimethylformamide (DMF) and 90% acetone, using natural phytoplankton communities in the Southern Ocean. Concentrations of chlorophyll α and phaeopigments were determined fluorometrically. The former solvent was employed during the 33rd Japanese Antarctic Research Expedition (JARE-33) while the latter has been used during previous JAREs. Statistical analyses showed that chlorophyll α concentrations were equivalent although the solvents were different. No significant difference was observed between concentrations of phaeopigments extracted with the different solvents either. The correlation coefficient obtained between phaeopigments concentrations was significantly lower than that between chlorophyll α concentrations. Phaeopigment concentrations extracted with DMF tended to be higher than those with 90% acetone. Total chlorophyllous pigments (chlorophyll α plus phaeopigments) were about 10% higher in the former solvent than in the latter, considering the slope of the regression line (=1.088). |
format |
Article in Journal/Newspaper |
author |
Tsuneo Odate Naomi Harada Mitsuo Fukuchi |
author_facet |
Tsuneo Odate Naomi Harada Mitsuo Fukuchi |
author_sort |
Tsuneo Odate |
title |
Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
title_short |
Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
title_full |
Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
title_fullStr |
Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
title_full_unstemmed |
Comparisons of N, N-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the SouthernOcean |
title_sort |
comparisons of n, n-dimethylformamide and 90% acetone as an extraction solvent for fluorometric determination of chlorophyllα from natural phytoplankton communities in the southernocean |
publisher |
National Institute of Polar Research |
publishDate |
1993 |
url |
https://doi.org/10.15094/00008821 https://doaj.org/article/cecaf63607e34d8996b69ac31477507d |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
Antarctic Record, Vol 37, Iss 3, Pp 260-264 (1993) |
op_relation |
doi:10.15094/00008821 0085-7289 2432-079X https://doaj.org/article/cecaf63607e34d8996b69ac31477507d |
op_rights |
undefined |
op_doi |
https://doi.org/10.15094/00008821 |
_version_ |
1766274731937366016 |