Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05
Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can...
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MDPI AG
2020
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Online Access: | https://doi.org/10.3390/su12176966 https://doaj.org/article/ac325bd8bcdd46238e8dc93ec5b899ee |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:ac325bd8bcdd46238e8dc93ec5b899ee 2023-05-15T14:04:32+02:00 Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 Mansur Abdulrasheed Azham Zulkharnain Nur Nadhirah Zakaria Ahmad Fareez Ahmad Roslee Khalilah Abdul Khalil Suhaimi Napis Peter Convey Claudio Gomez-Fuentes Siti Aqlima Ahmad 2020-08-01 https://doi.org/10.3390/su12176966 https://doaj.org/article/ac325bd8bcdd46238e8dc93ec5b899ee en eng MDPI AG doi:10.3390/su12176966 2071-1050 https://doaj.org/article/ac325bd8bcdd46238e8dc93ec5b899ee undefined Sustainability, Vol 12, Iss 6966, p 6966 (2020) Antarctica Arthrobacter diesel bioremediation growth kinetics response surface methodology geo envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.3390/su12176966 2023-01-22T19:00:04Z Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature. Article in Journal/Newspaper Antarc* Antarctic Antarctica Unknown Antarctic The Antarctic Sustainability 12 17 6966 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Antarctica Arthrobacter diesel bioremediation growth kinetics response surface methodology geo envir |
spellingShingle |
Antarctica Arthrobacter diesel bioremediation growth kinetics response surface methodology geo envir Mansur Abdulrasheed Azham Zulkharnain Nur Nadhirah Zakaria Ahmad Fareez Ahmad Roslee Khalilah Abdul Khalil Suhaimi Napis Peter Convey Claudio Gomez-Fuentes Siti Aqlima Ahmad Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
topic_facet |
Antarctica Arthrobacter diesel bioremediation growth kinetics response surface methodology geo envir |
description |
Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature. |
format |
Article in Journal/Newspaper |
author |
Mansur Abdulrasheed Azham Zulkharnain Nur Nadhirah Zakaria Ahmad Fareez Ahmad Roslee Khalilah Abdul Khalil Suhaimi Napis Peter Convey Claudio Gomez-Fuentes Siti Aqlima Ahmad |
author_facet |
Mansur Abdulrasheed Azham Zulkharnain Nur Nadhirah Zakaria Ahmad Fareez Ahmad Roslee Khalilah Abdul Khalil Suhaimi Napis Peter Convey Claudio Gomez-Fuentes Siti Aqlima Ahmad |
author_sort |
Mansur Abdulrasheed |
title |
Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_short |
Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_full |
Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_fullStr |
Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_full_unstemmed |
Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_sort |
response surface methodology optimization and kinetics of diesel degradation by a cold-adapted antarctic bacterium, arthrobacter sp. strain aq5-05 |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/su12176966 https://doaj.org/article/ac325bd8bcdd46238e8dc93ec5b899ee |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Sustainability, Vol 12, Iss 6966, p 6966 (2020) |
op_relation |
doi:10.3390/su12176966 2071-1050 https://doaj.org/article/ac325bd8bcdd46238e8dc93ec5b899ee |
op_rights |
undefined |
op_doi |
https://doi.org/10.3390/su12176966 |
container_title |
Sustainability |
container_volume |
12 |
container_issue |
17 |
container_start_page |
6966 |
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1766275701000896512 |