Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems
Nitrate (NO3−), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3− levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maxim...
Published in: | Ecotoxicology and Environmental Safety |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2021
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Online Access: | https://doi.org/10.1016/j.ecoenv.2020.111617 https://doaj.org/article/a29c5a369c2a48a3bb97fc9467c59443 |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:a29c5a369c2a48a3bb97fc9467c59443 2023-05-15T18:15:47+02:00 Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems Jiachen Yu Yanfeng Wang Yongshuang Xiao Xian Li Li Zhou Yunong Wang Tengfei Du Xiaona Ma Jun Li 2021-01-01 https://doi.org/10.1016/j.ecoenv.2020.111617 https://doaj.org/article/a29c5a369c2a48a3bb97fc9467c59443 en eng Elsevier 0147-6513 doi:10.1016/j.ecoenv.2020.111617 https://doaj.org/article/a29c5a369c2a48a3bb97fc9467c59443 undefined Ecotoxicology and Environmental Safety, Vol 208, Iss , Pp 111617- (2021) Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2021 fttriple https://doi.org/10.1016/j.ecoenv.2020.111617 2023-01-22T19:34:14Z Nitrate (NO3−), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3− levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3− levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose-dependent histopathological damage induced by NO3− exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3− significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRT-PCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3− exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3− level ... Article in Journal/Newspaper Scophthalmus maximus Turbot Unknown Ecotoxicology and Environmental Safety 208 111617 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity envir socio |
spellingShingle |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity envir socio Jiachen Yu Yanfeng Wang Yongshuang Xiao Xian Li Li Zhou Yunong Wang Tengfei Du Xiaona Ma Jun Li Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
topic_facet |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity envir socio |
description |
Nitrate (NO3−), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3− levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3− levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose-dependent histopathological damage induced by NO3− exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3− significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRT-PCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3− exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3− level ... |
format |
Article in Journal/Newspaper |
author |
Jiachen Yu Yanfeng Wang Yongshuang Xiao Xian Li Li Zhou Yunong Wang Tengfei Du Xiaona Ma Jun Li |
author_facet |
Jiachen Yu Yanfeng Wang Yongshuang Xiao Xian Li Li Zhou Yunong Wang Tengfei Du Xiaona Ma Jun Li |
author_sort |
Jiachen Yu |
title |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_short |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_full |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_fullStr |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_full_unstemmed |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_sort |
investigating the effect of nitrate on juvenile turbot (scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.ecoenv.2020.111617 https://doaj.org/article/a29c5a369c2a48a3bb97fc9467c59443 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Ecotoxicology and Environmental Safety, Vol 208, Iss , Pp 111617- (2021) |
op_relation |
0147-6513 doi:10.1016/j.ecoenv.2020.111617 https://doaj.org/article/a29c5a369c2a48a3bb97fc9467c59443 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.ecoenv.2020.111617 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
208 |
container_start_page |
111617 |
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1766189006946566144 |