Synthesis of Mesoporous Silica Nanowires and Their Application in Enzyme Immobilization

• Published in: E3S Web of Conferences
• Main Authors: Liu Peng, Liu Xinlong, An Na, Wang Peng
• Format: Article in Journal/Newspaper
• Language: English; French
• Published: EDP Sciences 2021
• Subjects: envir; geo; Antarc*; Antarctica
• Online Access: https://doi.org/10.1051/e3sconf/202124503006; https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/21/e3sconf_aeecs2021_03006.pdf; https://doaj.org/article/4141a34be5fb45dd9a13490f118df817

Hydrophobic mesoporous silica nanowires were synthesis and then employed as support for immobilization of lipase from Candida antarctica via covalent bonding (CALB@MSW). The parameters were optimized and the optimum conditions were as follows: GA concentration 5.5 wt.%, activation time 60 min and CALB concentration 4 mg/mL. Under these conditions, the protein loading and specific activity of CALB@MSW were 138.3 mg/gsupport and 41.1 U/mgsupport, respectively. Compared with free CALB, CALB@MSW showed better thermal stability and pH stability. The maximum yield of biodiesel catalytic by CALB@MSW was 93.4 %. After reused 8 times, CALB@MSW still remained 95.75 % initial activity showing better stability than free CALB.