Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher level...
Published in: | Ecotoxicology and Environmental Safety |
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2021
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Online Access: | https://doi.org/10.1016/j.ecoenv.2020.111846 https://doaj.org/article/1947871da41340db9aef5ce3122c11ab |
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fttriple:oai:gotriple.eu:oai:doaj.org/article:1947871da41340db9aef5ce3122c11ab 2023-05-15T14:51:16+02:00 Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic Helene Inderberg Eirik D. Neerland Molly McPartland Torfinn Sparstad Jenny Bytingsvik Vladimir A. Nikiforov Anita Evenset Åse Krøkje 2021-03-01 https://doi.org/10.1016/j.ecoenv.2020.111846 https://doaj.org/article/1947871da41340db9aef5ce3122c11ab en eng Elsevier 0147-6513 doi:10.1016/j.ecoenv.2020.111846 https://doaj.org/article/1947871da41340db9aef5ce3122c11ab undefined Ecotoxicology and Environmental Safety, Vol 210, Iss , Pp 111846- (2021) Anthropogenic contaminants Arctic Fish Genotoxicity Organochlorines envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2021 fttriple https://doi.org/10.1016/j.ecoenv.2020.111846 2023-01-22T18:09:57Z High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher levels of DNA double strand breaks (DSBs) than char from Lake Laksvatn. Even though there is increasing evidence of the genotoxic effects of OCs, little is known about the effects of OCs on the DNA repair system. The aim of the present study was to determine if the two main DNA DSB repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), are affected by the higher OC and DSB level in char from Ellasjøen. This was analysed by comparing the transcript level of 11 genes involved in DNA DSB repair in char liver samples from Ellasjøen (n = 9) with char from Laksvatn (n = 12). Six of the investigated genes were significantly upregulated in char from Ellasjøen. As the expression of DNA DSB repair genes was increased in the contaminant-exposed char, it is likely that the DNA DSB repair capacity is induced in these individuals. This induction was positively correlated with the DNA DSB and negatively correlated with one or several OCs for four of these genes. However, the strongest predictor variable for DNA repair genes was habitat, indicating genetic differences in repair capacity between populations. As char from Ellasjøen still had significantly higher levels of DSBs compared to char from Laksvatn, it is possible that chronic exposure to OCs and continued production of DSB has caused selective pressure within the population for fixation of adaptive alleles. It is also possible that DSB production was exceeding the repair capacity given the prevailing conditions, or that the OC or DSB level was above the threshold value of inhibition of the DNA repair system resulting in the rate of DNA damage exceeding the rate of repair. Article in Journal/Newspaper Arctic Bjørnøya Bjørnøya Salvelinus alpinus Unknown Arctic Norway Bjørnøya ENVELOPE(-67.250,-67.250,-68.151,-68.151) Ellasjøen ENVELOPE(19.031,19.031,74.388,74.388) Laksvatn ENVELOPE(19.360,19.360,69.361,69.361) Ecotoxicology and Environmental Safety 210 111846 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Anthropogenic contaminants Arctic Fish Genotoxicity Organochlorines envir |
spellingShingle |
Anthropogenic contaminants Arctic Fish Genotoxicity Organochlorines envir Helene Inderberg Eirik D. Neerland Molly McPartland Torfinn Sparstad Jenny Bytingsvik Vladimir A. Nikiforov Anita Evenset Åse Krøkje Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
topic_facet |
Anthropogenic contaminants Arctic Fish Genotoxicity Organochlorines envir |
description |
High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher levels of DNA double strand breaks (DSBs) than char from Lake Laksvatn. Even though there is increasing evidence of the genotoxic effects of OCs, little is known about the effects of OCs on the DNA repair system. The aim of the present study was to determine if the two main DNA DSB repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), are affected by the higher OC and DSB level in char from Ellasjøen. This was analysed by comparing the transcript level of 11 genes involved in DNA DSB repair in char liver samples from Ellasjøen (n = 9) with char from Laksvatn (n = 12). Six of the investigated genes were significantly upregulated in char from Ellasjøen. As the expression of DNA DSB repair genes was increased in the contaminant-exposed char, it is likely that the DNA DSB repair capacity is induced in these individuals. This induction was positively correlated with the DNA DSB and negatively correlated with one or several OCs for four of these genes. However, the strongest predictor variable for DNA repair genes was habitat, indicating genetic differences in repair capacity between populations. As char from Ellasjøen still had significantly higher levels of DSBs compared to char from Laksvatn, it is possible that chronic exposure to OCs and continued production of DSB has caused selective pressure within the population for fixation of adaptive alleles. It is also possible that DSB production was exceeding the repair capacity given the prevailing conditions, or that the OC or DSB level was above the threshold value of inhibition of the DNA repair system resulting in the rate of DNA damage exceeding the rate of repair. |
format |
Article in Journal/Newspaper |
author |
Helene Inderberg Eirik D. Neerland Molly McPartland Torfinn Sparstad Jenny Bytingsvik Vladimir A. Nikiforov Anita Evenset Åse Krøkje |
author_facet |
Helene Inderberg Eirik D. Neerland Molly McPartland Torfinn Sparstad Jenny Bytingsvik Vladimir A. Nikiforov Anita Evenset Åse Krøkje |
author_sort |
Helene Inderberg |
title |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_short |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_fullStr |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full_unstemmed |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_sort |
expression of dna repair genes in arctic char (salvelinus alpinus) from bjørnøya in the norwegian arctic |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.ecoenv.2020.111846 https://doaj.org/article/1947871da41340db9aef5ce3122c11ab |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(19.031,19.031,74.388,74.388) ENVELOPE(19.360,19.360,69.361,69.361) |
geographic |
Arctic Norway Bjørnøya Ellasjøen Laksvatn |
geographic_facet |
Arctic Norway Bjørnøya Ellasjøen Laksvatn |
genre |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
genre_facet |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
op_source |
Ecotoxicology and Environmental Safety, Vol 210, Iss , Pp 111846- (2021) |
op_relation |
0147-6513 doi:10.1016/j.ecoenv.2020.111846 https://doaj.org/article/1947871da41340db9aef5ce3122c11ab |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.ecoenv.2020.111846 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
210 |
container_start_page |
111846 |
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1766322321380868096 |